Transcript Profiling of Non‐Target‐Site Imidazolinone Resistance in Imisun Sunflower

Gil, Mercedes; Ochogavía, Ana Claudia; Vega, Tatiana Alejandra; Felitti, Silvina Andrea; Nestares, G.

doi:10.2135/cropsci2018.01.0074

Cited by 4 publications

(13 citation statements)

References 41 publications

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“…Recent transcriptomic characterization by complementary DNA‐amplified fragment length polymorphism has allowed detecting sequences related to imazethapyr metabolism in sunflower leaves that corresponded to four gene families: ABC transporters, glycosyltransferases, P450s, and UDP‐glucuronosyl/UDP‐glucosyltransferases (Gil, Ochogavía, et al., 2018). In addition, RNA sequencing analysis has allowed identifying detoxification genes potentially related to imazethapyr resistance in sunflower: cytochrome P450 monooxygenases, ABC transporters, UDP‐glucuronosyl and UDP‐glucosyltransferases, glycosyltransferases, and GSTs (Gil, Nestares, et al., 2018).…”

Section: Discussionmentioning

confidence: 99%

“…In addition to target‐site resistance (TSR) mechanisms, Imisun sunflowers present non‐target‐site resistance (NTSR) to IMI herbicides (Breccia et al., 2017; Gil, Nestares, et al., 2018; Gil, Ochogavía, Vega, Felitti, & Nestares, 2018; Sala et al., 2012). Although TSR mechanisms in sunflower are well described (Breccia, Vega, Felitti, Picardi, & Nestares, 2013; Kolkman et al., 2004; Sala et al., 2012; Vega et al., 2009, 2012), the nature of NTSR mechanisms has not yet been fully characterized but could be related to xenobiotic metabolism (Gil & Nestares, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…In particular, P450s have been shown to be involved in the metabolism of different types of herbicides in several crops and weed species (Siminszky, 2006; Yu & Powles, 2014) by carrying out in vivo experiments using P450 inhibitors such as tetcyclacis, 1‐aminobenzotriazole (ABT), piperonyl butoxide (PBO), and malathion (Beckie & Tardif, 2012; Letouzé & Gasquez, 2003; Yang et al., 2016). Previous studies involving plant growth response and transcriptomic analysis have described the participation of P450s and other gene families in the metabolism of herbicides in the genus Helianthus (Balabanova, Remans, Vassilev, Cuypers, & Vangronsveld, 2018; Breccia et al., 2017; Didierjean et al., 2002; Gil, Nestares, et al., 2018; Gil, Ochogavía, et al., 2018; Kaspar, Grondona, Leon, & Zambelli, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Stress response and detoxification mechanisms involved in non‐target‐site herbicide resistance in sunflower

et al. 2020

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The nature of non‐target‐site herbicide resistance (NTSR) to imidazolinone (IMI) in HA425 sunflower (Helianthus annuus L.) has not yet been fully characterized but could be related to xenobiotic metabolism. The objective of this study was to evaluate the role of cytochrome P450 monooxygenases (P450s) and other detoxification‐related proteins in NTSR in sunflower. Two sunflower inbred lines were used: HA 425, which is IMI resistant (Imisun), and HA 89, which is IMI susceptible. The growth response to the IMI herbicide imazethapyr in combination with the P450 inhibitors 1‐aminobenzotriazole (ABT) or piperonyl butoxide (PBO) was evaluated in 15‐d‐old sunflower plantlets. Roots were collected, and label‐free quantitation (LFQ) proteomic analysis was carried out to characterize the NTSR mechanisms involved in the IMI resistance trait in sunflower. The increased phytotoxicity of imazethapyr observed in the resistant line when ABT or PBO were present agrees with the hypothesis that NTSR mechanisms may contribute to herbicide resistance in sunflower. The herbicide treatment also led to changes in the levels of biotic and abiotic stress‐related proteins, glutathione S‐transferases, and cytochrome P450s, among others. Plant growth and root protein expression response to IMI herbicides in sunflower would be a combination of stress‐related and detoxification mechanisms. Understanding the basis of NTSR becomes helpful to exploit this trait in sunflower crop and to develop xenobiotic‐resistant, soil‐remediating cultivars.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Stress response and detoxification mechanisms involved in non‐target‐site herbicide resistance in sunflower

et al. 2020

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“…These constraints are overcame by alternative methods based on sequencing or PCR amplification (Breyne et al, 2003). A transcript profiling characterization of NTSR by cDNA-AFLP methodology was carried out in Imisun sunflower confirming the contribution of these mechanisms in imazethapyr resistance (Gil et al, 2018a) (Table 2). An important number of genes related to metabolism of xenobiotics and stress was found: P450 monooxygenases, UDP-glucuronosyl/UDP-glucosyltransferases, glycosyltransferases and ATP-binding cassette transporters, among others, suggesting that herbicide detoxification processes are involved in imidazolinone resistance in sunflower.…”

Section: High-throughput Approaches In Sunflowermentioning

confidence: 94%

“…Two mechanisms of AHAS-inhibitor herbicide resistance coexist in sunflower: an altered target site and enhanced herbicide metabolism (Sala et al, 2012b). The presence of NTSR mechanisms in sunflower resistant lines that are related specifically to herbicide metabolism has been suggested in several works (Balabanova et al, 2018;Breccia et al, 2017;Gil et al, 2018a;Kolkman et al, 2004) although very few genes endowing NTSR have been identified to date.…”

Section: Why To Focus On Ntsr In Sunflower?mentioning

confidence: 99%

Decoding Non-Target-Site Herbicide Resistance in Sunflower: The Beginning of the Story

Gil

Nestares

2019

Helia

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In the last years, many efforts have been made to develop sunflower cultivars showing important agronomical characteristics such as herbicide resistance. These approaches have been focused mainly on resistance to herbicides with the same mode of action, that is acetohydroxyacid synthase (AHAS) inhibitors. To date, four induced and natural AHAS mutations have been found that confer resistance to these herbicides and many of these alleles are being used for the production of sunflower hybrids resistant to herbicides and to develop different non-transgenic technologies for weed control. However, little is known about the bases of non-target-site-based resistance (NTSR) developing cross-resistance to herbicides with different modes of action in sunflower. These mechanisms diminish the number of active herbicide molecules that reach the target and are generally polygenic. Elucidating the nature of NTSR would allow evaluating maximal efficiency conditions for the herbicide and would enable to establish weed management strategies in sunflower crop. Nowadays, mining of NTSR genes can be more easily accomplished taking advantage of up-to-date omics-based approaches: high-throughput techniques involving genomics, transcriptomics, proteomics and metabolomics. Considering the difficulties in the discovery of new compounds with a broad spectrum of weed control, it results essential to broaden the use of former herbicides which are highly efficient and ecologically desirable. Full understanding of NTSR mechanisms in sunflower would allow detecting specific genes potentially useful as biotechnological tools for the phytoremediation of herbicides and modern plant breeding.

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Gene expression profiling by cDNA‐AFLP reveals potential candidate genes for imazapyr‐induced male sterility in sunflower

et al. 2021

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Chemically induced male sterility (CIMS) is a valuable tool for hybrid breeding in crops. In this context, the acetohydroxyacid synthase (AHAS)‐inhibiting imazapyr has been proposed to induce male sterility in Imisun sunflower (Helianthus annuus L.) when applied during early reproductive development in a two‐fold higher than recommended field dose. This study aimed to perform a comparative transcriptomic analysis to find out the molecular bases of the gametocidal effect of imazapyr on two Imisun genotypes differing in their herbicide resistance level: completely resistant and intermediate resistant. The CIMS of treated plants was confirmed at physiological and anatomical levels. A total of 948 differentially expressed transcript‐derived fragments (TDFs) were identified by complementary DNA amplified fragment‐length polymorphism (cDNA‐AFLP) analysis at the three most informative developmental stages of anther development. Several genes related to xenobiotic metabolism and stress, including cytochrome P450 monooxygenases and ATP‐binding cassette transporters, were identified in the completely resistant genotype. Interestingly, a group of genes, including protein kinases, sulfonylurea receptor 1, aspartic signal peptide‐peptidase, and specific membrane transporters, were recognized from the differential TDFs simultaneously detected in both genotypes. Some of these transcripts were validated by quantitative real‐time polymerase chain reaction (qPCR) in both genotypes. Therefore, these transcripts could be exclusively related to the CIMS in sunflower. Based on the results, we suggest a probable model of action for the gametocidal effect of imazapyr. Our research provides new insights into the molecular mechanisms of male sterility induction in sunflower.

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Transcript Profiling of Non‐Target‐Site Imidazolinone Resistance in Imisun Sunflower

Cited by 4 publications

References 41 publications

Stress response and detoxification mechanisms involved in non‐target‐site herbicide resistance in sunflower

Stress response and detoxification mechanisms involved in non‐target‐site herbicide resistance in sunflower

Decoding Non-Target-Site Herbicide Resistance in Sunflower: The Beginning of the Story

Gene expression profiling by cDNA‐AFLP reveals potential candidate genes for imazapyr‐induced male sterility in sunflower

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