Transcription activation by the adenovirus E1a protein

Lillie, James W.; Green, Michael R.

doi:10.1038/338039a0

Cited by 676 publications

(579 citation statements)

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“…pG5luc was constructed by subcloning the five UASg sites and TATA box from pG5E1bCAT (Lillie and Green, 1989) into the luciferase reporter plasmid pGL3basic (Promega, Madison, WI, USA). Plasmid pHLAB-luc was constructed by PCR amplification of the HLAB1 gene promoter from −284 to +1, which was then cloned into plasmid pGL3basic.…”

Section: Plasmid Dna Constructsmentioning

confidence: 99%

ZXDC, a novel zinc finger protein that binds CIITA and activates MHC gene transcription

Al-Kandari

Jambunathan

Navalgund

et al. 2007

Molecular Immunology

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The class II trans-activator (CIITA) is recognized as the master regulator of major histocompatibility complex (MHC) class II gene transcription and contributes to the transcription of MHC class I genes. To better understand the function of CIITA, we performed yeast two-hybrid with the C-terminal 807 amino acids of CIITA, and cloned a novel human cDNA named zinc finger, X-linked, duplicated family member C (ZXDC). The 858 amino acid ZXDC protein contains 10 zinc fingers and a transcriptional activation domain, and was found to interact with the region of CIITA containing leucine-rich repeats. Over-expression of ZXDC in human cell lines resulted in super-activation of MHC class I and class II promoters by CIITA. Conversely, silencing of ZXDC expression reduced the ability of CIITA to activate transcription of MHC class II genes. Given the specific interaction between the ZXDC and CIITA proteins, as well as the effect of ZXDC on MHC gene transcription, it appears that ZXDC is an important regulator of both MHC class I and class II transcription.

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Section: Plasmid Dna Constructsmentioning

confidence: 99%

ZXDC, a novel zinc finger protein that binds CIITA and activates MHC gene transcription

Al-Kandari

Jambunathan

Navalgund

et al. 2007

Molecular Immunology

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“…2). Neither the parent reporter plasmid [BCAT, which contains only a TATA box (42)] nor the internal control plasmid [copia-lacZ (35)] was affected by the expression of dE2F (data not shown and Fig. 2).…”

mentioning

confidence: 99%

DNA-binding and trans-activation properties of Drosophila E2F and DP proteins.

Dynlacht

Brook

Dembski

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

142

112

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The temporal activation of E2F ansptional activity appears to be an important component of the mechanisms that prepare m an cells for DNA replication. Regulation of E2F actvity appears to be a highly complex process, and the dissection of the E2F pathway will be greatly faclitated by the ability to use genetic approaches. [a-32P]dCTP by random primer extension (34) and used in low-stringency hybridization. Positive clones were plaque purified and cDNA inserts were subcloned into pBluescript SK (Stratagene) for sequencing. The inserts were digested with exonuclease III and S1 nuclease to generate nested sets of deletions, which were sequenced with Sequenase 2.0 (United States Biochemical). To isolate dDP, the same library was screened with a probe to the putative DNA-binding domain of DP-1 (17).Plasmids. pBS-dE2F was made by subcloning a 4.4-kb EcoRI fragment (the entire cDNA) from A phage 16 into pBluescript SK(+). pBS-dE2F.ATG is a modified form of pBS-dE2F constructed by use of PCR to delete the first 849 bp of pBS-dE2F. pBS-dDP was made by subcloning the entire cDNA insert on an EcoRI fragment from A phage 3 into EcoRI-cut pBluescript SK(+). The expression plasmid Act-PPA, the internal control plasmid copia-lacZ (35), and the chloramphenicol acetyltransferase (CAT) reporter (E2F)4-BCAT (17) used in transfection experiments have been described. Act-dE2F and Act-dDP contain the entire coding regions of the genes.Cell Culture and Transfections. Schneider line 2 (SL2) cells (36) (generously supplied by Jayne Kassel, Massachusetts General Hospital Cancer Center, Boston) were maintained at room temperature in Schneider's Drosophila medium and Abbreviations: GST, glutathione S-transferase; CAT, chloramphenicol acetyltransferase.

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“…The target vector p2GlucBG5 contains five Gal4-binding sites in the same position as the SV40 enhancer in p2GlucE. This plasmid was made by insertion of a blunt-ended PstI-XbaI fragment of pG5ElbCAT [37], bearing five Gal4 recognition sites, into the filled SaZI site of p2GlucB, 2.85 kbp downstream of the transcription start site. The structure of the constructs was confirmed by restriction analysis and DNA sequencing.…”

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confidence: 99%

Transcriptional Activation and Repression, Two Properties of the Lymphoid‐Specific Transcription Factor Oct‐2a

Friedl

Matthias

1995

European Journal of Biochemistry

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The lymphoid-specific transcription factor Oct-2a contains two transcriptional activation domains which are located within the N-terminal and C-terminal regions. To study their differential activation properties, we linked the isolated effector domains to the GAL4 DNA-binding domain. We have shown that both activating regions of Oct-2a, isolated from their natural context, can activate transcription as promoter factors. In contrast to the C-terminus, activation by the N-terminal domain is dependent on a yet unidentified factor(s) binding to the simian virus 40 enhancer. The results obtained by duplication of activation domains or their mixed combination suggest that the domains are functionally independent. However, activation from a remote position could only be achieved with the C-terminus of Oct-2a in B cells. In lymphoid cells, higher activation levels were observed, suggesting that distinct B-cell-specific cofactors in concert with the effector domains of Oct-2a might be involved in mediating transcription from proximal and remote positions. Furthermore, we identified a repression domain at the N-terminus of Oct-2a. When transferred to a potent activator, transcriptional stimulation was inhibited efficiently. These results underscore the modular structure of Oct-2a with separable domains for activation and repression and suggest that Oct-2a might have complex regulatory functions in vivo.

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Transcription activation by the adenovirus E1a protein

Cited by 676 publications

References 56 publications

ZXDC, a novel zinc finger protein that binds CIITA and activates MHC gene transcription

ZXDC, a novel zinc finger protein that binds CIITA and activates MHC gene transcription

DNA-binding and trans-activation properties of Drosophila E2F and DP proteins.

Transcriptional Activation and Repression, Two Properties of the Lymphoid‐Specific Transcription Factor Oct‐2a

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