Transcription Activator-Like Effector (TALE) Nucleases and Repressor TALEs for Antiviral Gene Therapy

Bloom, Kristie; Mussolino, Claudio; Arbuthnot, Patrick

doi:10.1007/s40778-014-0008-7

Cited by 11 publications

(6 citation statements)

References 52 publications

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“…Ứng dụng công nghệ TALEN trong việc tạo tính kháng virus trên thực vật chưa được ghi nhận. Tuy nhiên, hệ thống này đã được khẳng định tiềm năng trong việc chống lại một số loại virus gây bệnh trên người như virus viêm gan B (HBV), viêm gan C (HCV) và virus suy giảm miễn dịch ở người (HIV) (Bloom et al, 2015).…”

Section: Tạo Tính Kháng Virus Bằng Công Nghệ Zfn Hoặc Talenunclassified

Methods to manage, protect and improve plant virus resistance

Tien¹,

Ngoc²,

Hà³

2022

Vietnam J. Biotechnol.

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Viral diseases caused severe damage for plant growth and development. Penetration and spread of viruses in the host plants dramatically reduce crop yield and quality. Due to the critical damages of viral diseases to agricultural production, different approaches and methods have been developed and utilized to manage, protect and improve plant virus resistance. Of which, conventional methods such as meristem culture, thermotherapy, cryotherapy as well as chemotherapy have been widely used and conducted effective results. Moreover, cross protection and gene pyramiding approaches have performed the broad and stable spectrum resistance potential in different plant species. Recently, advanced methods like plant transformation, gene silencing as well as genome editing have shown great successes in plant virus resistant improvement. In this review, we will briefly introduce the principle, advantages and limitations of different methods used for plant viral diseases management and viral resistant improvements. In addition, we will also discuss the challenges and future aspects in utilizing advanced technologies for plant virus resistant enhancement and breeding.

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Section: Tạo Tính Kháng Virus Bằng Công Nghệ Zfn Hoặc Talenunclassified

Methods to manage, protect and improve plant virus resistance

Tien¹,

Ngoc²,

Hà³

2022

Vietnam J. Biotechnol.

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“…TALEs are specific, easy to design and generally less cytotoxic than other DNA targeting proteins [206]. On the downside, TALEs are relatively large and contain repetitive sequences that can complicate cloning [211] and interfere with incorporation into gene delivery vectors [212,213].…”

Section: Talesmentioning

confidence: 99%

Reduce and Control: A Combinatorial Strategy for Achieving Sustained HIV Remissions in the Absence of Antiretroviral Therapy

Schwarzer

Gramatica

Greene

2020

Viruses

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Human immunodeficiency virus (HIV-1) indefinitely persists, despite effective antiretroviral therapy (ART), within a small pool of latently infected cells. These cells often display markers of immunologic memory and harbor both replication-competent and -incompetent proviruses at approximately a 1:100 ratio. Although complete HIV eradication is a highly desirable goal, this likely represents a bridge too far for our current and foreseeable technologies. A more tractable goal involves engineering a sustained viral remission in the absence of ART--a "functional cure." In this setting, HIV remains detectable during remission, but the size of the reservoir is small and the residual virus is effectively controlled by an engineered immune response or other intervention. Biological precedence for such an approach is found in the post-treatment controllers (PTCs), a rare group of HIV-infected individuals who, following ART withdrawal, do not experience viral rebound. PTCs are characterized by a small reservoir, greatly reduced inflammation, and the presence of a poorly understood immune response that limits viral rebound. Our goal is to devise a safe and effective means for replicating durable post-treatment control on a global scale. This requires devising methods to reduce the size of the reservoir and to control replication of this residual virus. In the following sections, we will review many of the approaches and tools that likely will be important for implementing such a "reduce and control" strategy and for achieving a PTC-like sustained HIV remission in the absence of ART.

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“…Targeting viral DNA or host factors associated with pathogenesis of viral disease for disruption could be the possible strategies for virus suppression and disease resistance. There is a great possibility and progress in the idea of using TALEN and TALE repressors for antiviral gene therapy as well, to suppress potent viruses that cause global mortality and morbidity like HIV [90]. So far, different regions of viral genomes have been targeted to inhibit replication and to suppress viruses.…”

Section: Use Of Gened Tools Against Biotic Stresses In Cottonmentioning

confidence: 99%

Targeted Genome Editing for Cotton Improvement

Khan¹,

Khan²,

Mubarik³

et al. 2018

Past, Present and Future Trends in Cotton Breeding

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Conventional tools induce mutations randomly throughout the cotton genome-making breeding difficult and challenging. During the last decade, progress has been made to edit the gene of interest in a very precise manner. Targeted genome engineering with engineered nucleases (ENs) specifically zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided nucleases (e.g., Cas9) has been described as a "game-changing technology" for diverse fields as human genetics and plant biotechnology. In eukaryotic systems, ENs create double-strand breaks (DSBs) at the targeted DNA sequence which are repaired by nonhomologous end joining (NHEJ) or homologydirected recombination (HDR) mechanisms. ENs have been used successfully for targeted mutagenesis, gene knockout, and multisite genome editing (GenEd) in model plants and crop plants such as cotton, rice, and wheat. Recently, cotton genome has also been edited for targeted mutagenesis through CRISPR/Cas for improved lateral root formation. In addition, an efficient and fast method has been developed to evaluate guide RNAs transiently in cotton. The targeted disruption of undesirable genes or metabolic pathway can be achieved to increase quality of cotton. Undesirable metabolites like gossypol in cottonseed can be targeted efficiently using ENs for seed-specific low-gossypol cotton. Moreover, ENs are also helpful in gene stacking for herbicide resistance, insect resistance, and abiotic stress tolerance.

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Transcription Activator-Like Effector (TALE) Nucleases and Repressor TALEs for Antiviral Gene Therapy

Cited by 11 publications

References 52 publications

Methods to manage, protect and improve plant virus resistance

Methods to manage, protect and improve plant virus resistance

Reduce and Control: A Combinatorial Strategy for Achieving Sustained HIV Remissions in the Absence of Antiretroviral Therapy

Targeted Genome Editing for Cotton Improvement

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