Transcription Analysis of the Chemerin Impact on Gene Expression Profile in the Luteal Cells of Gilts

Makowczenko, Karol G; Jastrzebski, J; Szeszko, Karol; Smolińska, Nina; Paukszto, Łukasz; Kieżun, Marta; Rytelewska, Edyta; Kamińska, Barbara; Kamiński, Tadeusz

doi:10.3390/genes11060651

Cited by 8 publications

(26 citation statements)

References 81 publications

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“…Activation of AMPK, homeostatic regulator of cellular energy levels, enhances glycolysis by phosphorylation and activation of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases-3 (PFKFB3), ultimately protecting from death [62]. Chemerin was demonstrated to upregulate gene encoding PFKFB3 protein [63]. PFKFB3, also regulated by HIF-1α, was reported to be upregulated in multiple cancer types, where it was required for their survival and growth [64].…”

Section: Discussionmentioning

confidence: 99%

Association of the Adipokines Chemerin, Apelin, Vaspin and Omentin and Their Functional Genetic Variants with Rheumatoid Arthritis

Wahba

Ibrahim

Abo-Elmatty

et al. 2021

JPM

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Adipokines were shown to exert crucial roles in rheumatic diseases. This study aimed to assess the role of chemerin, apelin, vaspin, and omentin adipokines and their genetic variants rs17173608, rs2235306, rs2236242, and rs2274907, respectively, in rheumatoid arthritis (RA) pathogenesis in Egyptian patients. A total of 150 RA patients and 150 healthy individuals were recruited. Blood samples were collected and used for genotyping. Serum was separated and used for expression analysis by quantitative PCR, and various biochemical markers determination by ELISA. Serum protein levels of chemerin and vaspin, as well as their gene expression levels were higher, while those of apelin and omentin were lower in RA patients and were associated with most of RA clinical and laboratory characteristics. G allele of chemerin rs17173608, T allele of vaspin rs2236242, and T allele of omentin rs2274907 were more frequent in RA patients. Serum levels and gene expression levels of chemerin in GG genotype carriers and vaspin in TT genotype group were significantly higher, while those of omentin in TT genotype carriers were significantly lower than RA patients with other genotypes. There was no association between apelin rs2235306 and RA. Chemerin rs17173608, vaspin rs2236242, and omentin rs2274907 polymorphisms were associated with increased susceptibility to RA.

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Section: Discussionmentioning

confidence: 99%

Association of the Adipokines Chemerin, Apelin, Vaspin and Omentin and Their Functional Genetic Variants with Rheumatoid Arthritis

Wahba

Ibrahim

Abo-Elmatty

et al. 2021

JPM

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“…Among all RNA-Seq libraries used in this study, the average number of reads’ bases mapped to the coding DNA sequences (CDS) were 59.52%, untranslated regions (UTR) were 23.32%, intronic regions were 3.35%, and intergenic locations were 13.81%. The detailed statistics of the sequencing and pre-processing data, principal component analysis and sample-to-sample distance matrix were summarized by Makowczenko et al [ 19 ].…”

Section: Resultsmentioning

confidence: 99%

“…Counts per gene and per transcript were computed using the prepDE Python script provided by the StringTie’s authors [ 60 ]. The DEGs analysis process was conducted using the Ballgown tool v. 2.18.0 [ 61 ], following the procedure described by Makowczenko et al [ 19 ], with the operating parameters: q -value < 0.05 and |log2FC| ≥ 0.5.…”

Section: Methodsmentioning

confidence: 99%

“…Despite extensive research analysing the general mechanisms of CHEM action, to date, the details of its influence at the molecular level on specific cells resulting in the modulation of transcription and translation processes remains poorly described. This manuscript is the extension of our previously published analyses of transcriptional profiles on the same research model [ 19 ]. Moreover, we have analysed the results obtained by our team using other laboratory techniques on identical research models [ 17 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 98%

“…We have recently described the effects of CHEM on the transcriptomic profile of porcine luteal cells (LCs) and direct implications for the CLs secretory functions. We have shown CHEM effects on steroidogenesis and prostaglandin synthesis essential for ovarian function, on activation of NF-κB and Jak/STAT signalling pathways, pro- and anti-apoptotic activities, as well as angiogenesis [ 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

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Chemerin Impact on Alternative mRNA Transcription in the Porcine Luteal Cells

Makowczenko

Jastrzebski

Paukszto

et al. 2022

Cells

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Chemerin participates in the regulation of processes related to physiological and disorder mechanisms in mammals, including metabolism, obesity, inflammation, and reproduction. In this study, we have investigated chemerin influence on alternative mRNA transcription within the porcine luteal cell transcriptome, such as differential expression of long non-coding RNAs (DELs) and their interactions with differentially expressed genes (DEGs), differences in alternative splicing of transcripts (DASs), and allele-specific expression (ASEs) related to the single nucleotide variants (SNVs) frequency. Luteal cells were collected from gilts during the mid-luteal phase of the oestrous cycle. After in vitro culture of cells un-/treated with chemerin, the total RNA was isolated and sequenced using the high-throughput method. The in silico analyses revealed 24 DELs cis interacting with 6 DEGs and trans-correlated with 300 DEGs, 137 DASs events, and 18 ASEs. The results enabled us to analyse metabolic and signalling pathways in detail, providing new insights into the effects of chemerin on the corpus luteum functions related to inflammatory response, leukocyte infiltration, the occurrence of luteotropic and luteolytic signals (leading to apoptosis and/or necroptosis). Validation of the results using qPCR confirmed the predicted expression changes. Chemerin at physiological concentrations significantly modifies the transcription processes in the porcine luteal cells.

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Chemerin as a modulator of angiogenesis and apoptosis processes in the corpus luteum of pigs: an in vitro study

Rytelewska

Kieżun

Zaobidna

et al. 2021

Biology of Reproduction

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The corpus luteum (CL) undergoes rapid changes, and its functional capabilities are influenced by processes such as angiogenesis and apoptosis. According to the literature, chemerin – a protein which participates in the regulation of energy homeostasis and the immune response, may also affect angiogenesis and apoptosis. Therefore, the aim of this study was to investigate the in vitro effect of chemerin on angiogenesis and apoptosis in porcine luteal cells (Lc) during specific phases related to CL physiology. Luteal cells were harvested from gilts during the early-, mid-, and late-luteal phases of the estrous cycle. The cells were preincubated for 48 h and incubated for 24 h with chemerin or a serum-free medium (controls). The abundance of angiogenesis- and apoptosis-related proteins was determined by ELISA in spent culture media, or by ELISA and Western Blot in protein extracts. The current study demonstrated that chemerin stimulates the production of VEGF-A and bFGF by porcine Lc and increases the protein abundance of angiogenic factors receptors (VEGFR1, VEGFR2, VEGFR3, FGFR1, FGFR2) in these cells. The study also revealed that chemerin exerts a modulatory effect (stimulatory/inhibitory, depending on the phase of the cycle) on the protein abundance of Fas, FasL, Bcl-2 and caspase-3 in porcine Lc. These results imply that chemerin may affect angiogenesis and apoptosis processes in the porcine CL, as evidenced by its modulatory effect of chemerin on the protein abundance of crucial angiogenesis- and apoptosis-related factors, observed in an in vitro study of porcine Lc.

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Transcription Analysis of the Chemerin Impact on Gene Expression Profile in the Luteal Cells of Gilts

Cited by 8 publications

References 81 publications

Association of the Adipokines Chemerin, Apelin, Vaspin and Omentin and Their Functional Genetic Variants with Rheumatoid Arthritis

Association of the Adipokines Chemerin, Apelin, Vaspin and Omentin and Their Functional Genetic Variants with Rheumatoid Arthritis

Chemerin Impact on Alternative mRNA Transcription in the Porcine Luteal Cells

Chemerin as a modulator of angiogenesis and apoptosis processes in the corpus luteum of pigs: an in vitro study

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