Transcription and its regulation in mammalian and human mitochondria

Sologub, Marina; Kochetkov, S. N.; Temiakov, Dmitry

doi:10.1134/s0026893309020034

Cited by 4 publications

(2 citation statements)

References 110 publications

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“…Importantly, a wealth of biochemical information exists pertaining to certain aspects of the organization of the complex, including details of specific protein–protein and protein–DNA interactions ( 49 , 50 ). In addition, both the initiation site and TFAM-binding sites have been clearly defined, ( 51 ) and together, these details provide an important set of biochemical constraints that should be recapitulated by a valid docking model.…”

Section: Resultsmentioning

confidence: 99%

Organization of the human mitochondrial transcription initiation complex

Yakubovskaya

Guja

Eng

et al. 2014

Nucleic Acids Research

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Initiation of transcription in human mitochondria involves two factors, TFAM and TFB2M, in addition to the mitochondrial RNA polymerase, POLRMT. We have investigated the organization of the human mitochondrial transcription initiation complex on the light-strand promoter (LSP) through solution X-ray scattering, electron microscopy (EM) and biochemical studies. Our EM results demonstrate a compact organization of the initiation complex, suggesting that protein–protein interactions might help mediate initiation. We demonstrate that, in the absence of DNA, only POLRMT and TFAM form a stable interaction, albeit one with low affinity. This is consistent with the expected transient nature of the interactions necessary for initiation and implies that the promoter DNA acts as a scaffold that enables formation of the full initiation complex. Docking of known crystal structures into our EM maps results in a model for transcriptional initiation that strongly correlates with new and existing biochemical observations. Our results reveal the organization of TFAM, POLRMT and TFB2M around the LSP and represent the first structural characterization of the entire mitochondrial transcriptional initiation complex.

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Section: Resultsmentioning

confidence: 99%

Organization of the human mitochondrial transcription initiation complex

Yakubovskaya

Guja

Eng

et al. 2014

Nucleic Acids Research

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“…Mitochondria are the main sites for respiration and inherit maternally [5,6]. Hitherto, prodigious work on mitochondria revealed that mtDNA mutations may cause type 2 diabetes mellitus.…”

Section: Introductionmentioning

confidence: 99%

Detection of 3243 A/G and 3316 G/A mitochondrial DNA mutations in Nagpur population

2020

jabb

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The present study aims to detect 3243 A/G and 3316 G/A mitochondrial DNA (mtDNA) mutations in Nagpur population. Total of 142 patients of type 2 diabetes mellitus and 142 healthy control individuals were selected for the study from Nagpur city. Selected mutations studied using restriction fragment length polymorphism method and confirmed by DNA sequencing. Results showed that 3316 G/A mt DNA mutation found in seven patients of type 2 diabetes mellitus with a 4.92% prevalence, however, found absent in healthy control individuals. Chi-Square and Fisher's exact test showed a significant association between healthy control individuals and type 2 diabetes mellitus patients detected with 3316 G/A mutation (p ≤ 0.01). ODDS ratio found significant (for 95% CI; p = 0.05) for 3316 G/A mutation. Furthermore, we did not find 3243 A/G mtDNA mutation in the studied population. Among studied mutations, 3316 G/A mutation in the ND1 gene is a pathogenic mutation that may responsible for type 2 diabetes mellitus in the Nagpur population.

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Structural dynamics of the active center of multisubunit RNA polymerases during RNA synthesis and proofreading

Pupov

Kulbachinskiy

2010

Mol Biol

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Transcription and its regulation in mammalian and human mitochondria

Cited by 4 publications

References 110 publications

Organization of the human mitochondrial transcription initiation complex

Organization of the human mitochondrial transcription initiation complex

Detection of 3243 A/G and 3316 G/A mitochondrial DNA mutations in Nagpur population

Structural dynamics of the active center of multisubunit RNA polymerases during RNA synthesis and proofreading

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