Transcription elements AREB6 and miR-34a affect apoptosis of PAMs by regulating the expression of SS2-related gene PPP1R11

Abstract

In our previous work, gene PPP1R11 (protein phosphatase 1 regulatory subunit 11) was significantly expressed in pigs after Streptococcus suis 2 (SS2) challenged. This study firstly confirmed that SS2 induced significant expression of PPP1R11 gene in porcine alveolar macrophage (PAM) cells, and apoptosis of PAM cells were observed. After that, the core promoter of porcine PPP1R11 was identified and its transcription factor AREB6 which significantly regulated PPP1R11. We also characterized that the PPP1R11 gene is a target of miR-34a. Further, we found that PPP1R11 helped to inhibit apoptosis of PAM cells under SS2 infecting, through transcription factor AREB6 was negatively correlated with apoptosis whereas miR-34a was positively correlated. Those findings provide a functional connection among the transcription factor AREB6, miR-34a, PPP1R11 gene and apoptosis of PAM cells in the pathogenesis of the SS2 infection.

Analysis of PPP1R11 expression in granulosa cells during developmental follicles of yak and its effects on cell function

“…After interference, the transcription level of the apoptosis‐promoting BAX , P53 and CASP3 increased significantly in GCs, while the transcription level of the anti‐apoptotic BCL2 gene was not significantly decreased. Moreover, our findings demonstrate that the ratio of BAX/BCL2 was negatively correlated with PPP1R11 expression, consistent with previous study (Liu et al, 2019). These preliminary results indicate that PPP1R11 interference changes the gene transcription level of genes involved in the apoptosis signalling pathway, thereby affecting the activity of the pathway.…”