Transcription elongation factor AtSPT4-2 positively modulates salt tolerance in Arabidopsis thaliana

Liaqat, Ayesha; Alfatih, Alamin; Jan, Sami Ullah; Sun, Liangqi; Zhao, Ping‐Xia; Xiang, Cheng‐Bin

doi:10.1186/s12870-023-04060-x

Cited by 6 publications

(1 citation statement)

References 71 publications

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“…It also activates the positive regulator of ABA signaling (SnRK2) (Katsuta et al, 2020), phosphorylates downstream ABF, and regulates the expression of downstream ABA‐responsive genes, thereby affecting plant responses to abiotic stress (Nishimura et al, 2018). In addition, osmotic pressure is maintained by SnRK2s and is activated by group B Raf‐like protein kinases, which promote plant growth and development independent of ABA (Kamiyama et al, 2021; Liaqat et al, 2023). In Vitis vinifera , 3 genes (1 gene encoding PYL4 and 2 genes encoding PYL2) were observed to be down‐regulated under salt stress (Lu et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Integrative multi‐omics analysis reveals the crucial biological pathways involved in the adaptive response to NaCl stress in peanut seedlings

Zhang,

et al. 2024

Physiologia Plantarum

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Plant growth is restricted by salt stress, which is a significant abiotic factor, particularly during the seedling stage. The aim of this study was to investigate the mechanisms underlying peanut adaptation to salt stress by transcriptomic and metabolomic analysis during the seedling stage. In this study, phenotypic variations of FH23 and NH5, two peanut varieties with contrasting tolerance to salt, changed obviously, with the strongest differences observed at 24 h. FH23 leaves wilted and the membrane system was seriously damaged. A total of 1470 metabolites were identified, with flavonoids being the most common (21.22%). Multi‐omics analyses demonstrated that flavonoid biosynthesis (ko00941), isoflavones biosynthesis (ko00943), and plant hormone signal transduction (ko04075) were key metabolic pathways. The comparison of metabolites in isoflavone biosynthesis pathways of peanut varieties with different salt tolerant levels demonstrated that the accumulation of naringenin and formononetin may be the key metabolite leading to their different tolerance. Using our transcriptomic data, we identified three possible reasons for the difference in salt tolerance between the two varieties: (1) differential expression of LOC112715558 (HIDH) and LOC112709716 (HCT), (2) differential expression of LOC112719763 (PYR/PYL) and LOC112764051 (ABF) in the abscisic acid (ABA) signal transduction pathway, then (3) differential expression of genes encoding JAZ proteins (LOC112696383 and LOC112790545). Key metabolites and candidate genes related to improving the salt tolerance in peanuts were screened to promote the study of the responses of peanuts to NaCl stress and guide their genetic improvement.

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Section: Discussionmentioning

confidence: 99%

Integrative multi‐omics analysis reveals the crucial biological pathways involved in the adaptive response to NaCl stress in peanut seedlings

Zhang,

et al. 2024

Physiologia Plantarum

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Natural variation in SbTEF1 contributes to salt tolerance in sorghum seedlings

Liu,

Tian,

et al. 2024

Journal of Integrative Agriculture

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Different elongation factors distinctly modulate RNA polymerase II transcription in Arabidopsis

Obermeyer,

Schrettenbrunner,

Stöckl

et al. 2023

Nucleic Acids Research

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Various transcript elongation factors (TEFs) including modulators of RNA polymerase II (RNAPII) activity and histone chaperones tune the efficiency of transcription in the chromatin context. TEFs are involved in establishing gene expression patterns during growth and development in Arabidopsis, while little is known about the genomic distribution of the TEFs and the way they facilitate transcription. We have mapped the genome-wide occupancy of the elongation factors SPT4–SPT5, PAF1C and FACT, relative to that of elongating RNAPII phosphorylated at residues S2/S5 within the carboxyterminal domain. The distribution of SPT4–SPT5 along transcribed regions closely resembles that of RNAPII-S2P, while the occupancy of FACT and PAF1C is rather related to that of RNAPII-S5P. Under transcriptionally challenging heat stress conditions, mutant plants lacking the corresponding TEFs are differentially impaired in transcript synthesis. Strikingly, in plants deficient in PAF1C, defects in transcription across intron/exon borders are observed that are cumulative along transcribed regions. Upstream of transcriptional start sites, the presence of FACT correlates with nucleosomal occupancy. Under stress conditions FACT is particularly required for transcriptional upregulation and to promote RNAPII transcription through +1 nucleosomes. Thus, Arabidopsis TEFs are differently distributed along transcribed regions, and are distinctly required during transcript elongation especially upon transcriptional reprogramming.

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Transcription elongation factor AtSPT4-2 positively modulates salt tolerance in Arabidopsis thaliana

Cited by 6 publications

References 71 publications

Integrative multi‐omics analysis reveals the crucial biological pathways involved in the adaptive response to NaCl stress in peanut seedlings

Integrative multi‐omics analysis reveals the crucial biological pathways involved in the adaptive response to NaCl stress in peanut seedlings

Natural variation in SbTEF1 contributes to salt tolerance in sorghum seedlings

Different elongation factors distinctly modulate RNA polymerase II transcription in Arabidopsis

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