Transcription Factor ERF194 Modulates the Stress-Related Physiology to Enhance Drought Tolerance of Poplar

Huan, Xuhui; Wang, Xingqi; Zou, Shengqiang; Zhao, Kai; Han, Youzhi; Wang, Shengji

doi:10.3390/ijms24010788

Cited by 13 publications

(3 citation statements)

References 53 publications

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“…The functions of TFs in conferring tolerance to drought stress in woody crops have been extensively analyzed. Overexpression of ERF genes has been shown to enhance tolerance to drought stress in woody crops [33,34]. Additionally, MdHB-7, which encodes an HD-Zip TF, was found to promote drought tolerance in apple [35].…”

Section: Discussionmentioning

confidence: 99%

Identification of drought stress-responsive genes in Korean fir (Abies koreana) through comparative RNA-seq analysis

Park,

Hwang

2024

Preprint

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Background Abies koreana, an endangered species sensitive to drought, faces severe decline due to climate change-induced water deficit. Results In this study, we performed a comprehensive transcriptomic analysis of A. koreana under drought stress. RNA-seq data analysis revealed 85,403 contigs of extended lengths, indicative of a high-quality assembly. Gene Ontology classification revealed strong drought stress-responsive regulation of unigenes associated with various biological processes, cellular components, and molecular functions. Gene expression levels showed variation at different time points, indicating a nuanced plant response. Numerous transcription factors, notably those belonging to ERF, MYB, LBD, and NAC families, were identified, underscoring their critical roles in drought tolerance. Finally, qRT-PCR validation confirmed the reliability of RNA-seq data. Conclusion Overall, this study reveals candidate genes required for drought tolerance in A. koreana, thus supporting future tree breeding programs for effective forest management.

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Section: Discussionmentioning

confidence: 99%

Identification of drought stress-responsive genes in Korean fir (Abies koreana) through comparative RNA-seq analysis

Park,

Hwang

2024

Preprint

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“…MDA content was determined using the colorimetric method using barbituric acid. Root vitality was determined using the triphenyltetrazolium chloride (TTC) method [56,57]. At least six biological replicates were performed for each experiment.…”

Section: Determination Of Physiological Indicatorsmentioning

confidence: 99%

PagMYB205 Negatively Affects Poplar Salt Tolerance through Reactive Oxygen Species Scavenging and Root Vitality Modulation

Zhou,

Huan,

Zhao

et al. 2023

IJMS

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Salt stress is one of the major abiotic stresses that limits plant growth and development. The MYB transcription factor family plays essential roles in plant growth and development, as well as stress tolerance processes. In this study, the cDNA of the 84K poplar (Populus abla × Populus glandulosa) was used as a template to clone the full length of the PagMYB205 gene fragment, and transgenic poplar lines with PagMYB205 overexpression (OX) or inhibited expression (RNAi, RNA interference) were cultivated. The role of PagMYB205 in poplar growth and development and salt tolerance was detected using morphological and physiological methods. The full-length CDS sequence of PagMYB205 was 906 bp, encoding 301 amino acids, and the upstream promoter sequence contained abiotic stress-related cis-acting elements. The results of subcellular localization and transactivation assays showed that the protein had no self-activating activity and was localized in the nucleus. Under salt stress, the rooting rate and root vitality of RNAi were higher than OX and wild type (WT). However, the malondialdehyde (MDA) content of the RNAi lines was significantly lower than that of the wild-type (WT) and OX lines, but the reactive oxygen species (ROS) scavenging ability, such as the peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) enzyme activities, was dramatically more powerful. Most significantly of all, the RNAi3 line with the lowest expression level of PagMYB205 had the lowest MDA content, the best enzyme activity and root vitality, and the best salt stress tolerance compared to the other lines. The above results suggest that the transcription factor PagMYB205 could negatively regulate salt stress tolerance by regulating antioxidant enzyme activity and root vitality.

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“…The SOD assay employed the more stable and sensitive WST-8 method, which could react with the superoxide anion (O 2− ) produced by catalyzing xanthine oxidase (XO) to generate a water-soluble formazan dye [27]. POD and CAT activities were measured using the guaiacol and UV spectrophotometric methods, respectively [28]. The detection of H 2 O 2 content utilized the reaction of H 2 O 2 with titanium salt to generate a peroxide-titanium complex purple precipitate, which can be dissolved in concentrated sulfuric acid and then detected using a UV spectrophotometer at a maximum absorption peak at a 415 nm wavelength.…”

Section: Determination Of the Physiological And Biochemical Parameter...mentioning

confidence: 99%

Hub Gene Identification and Heat-Stress-Related Transcriptional Regulation Mechanism in Cabbage (Brassica oleracea L.)

Li,

Zhang,

Zhao

et al. 2023

Horticulturae

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Cabbage is a heat-stress-sensitive cool-season crop. When exposed to high temperatures, cabbage plants can experience reduced growth, wilting, leaf yellowing, and premature bolting (the formation of a flowering stem). The regulatory mechanism controlling heat stress is poorly understood in cabbage. To investigate this mechanism, physiological changes and transcriptional profiling for different heat treatment times were analyzed in this study. The results showed that superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities were enhanced under heat stress. In particular, the increase in SOD and POD activities after 12 h of heat treatment was greater than that after 4 h of heat treatment. With increasing heat treatment duration, the leaf CAT activity and H2O2 content decreased after the initial increase. The electrolyte leakage and malondialdehyde (MDA) content dropped significantly, while the proline content increased. Alongside that, 7007 and 5537 upregulated genes were identified in the experimental group treated with heat stress for the 4 h and 12 h treatments, respectively. We found that 10,479 DEGs were shared in the heat stress treatment, of which 1241 were associated with the heat treatment time. By integrating the expression patterns and functional annotations of genes related to heat stress, we identified 15 hub genes that respond to heat stress in cabbage. Meanwhile, we had constructed a physiological to molecular model of cabbage response to long-term heat stress. These findings provide new insights for the comprehensive analysis of cabbage response to heat stress and genetic resources for breeding new varieties of cabbage with heat tolerance via genetic engineering.

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Transcription Factor ERF194 Modulates the Stress-Related Physiology to Enhance Drought Tolerance of Poplar

Cited by 13 publications

References 53 publications

Identification of drought stress-responsive genes in Korean fir (Abies koreana) through comparative RNA-seq analysis

Identification of drought stress-responsive genes in Korean fir (Abies koreana) through comparative RNA-seq analysis

PagMYB205 Negatively Affects Poplar Salt Tolerance through Reactive Oxygen Species Scavenging and Root Vitality Modulation

Hub Gene Identification and Heat-Stress-Related Transcriptional Regulation Mechanism in Cabbage (Brassica oleracea L.)

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