Transcription Factor 
 SiNF-YA5 from Foxtail Millet ( 
 Setaria italica ) Conferred Tolerance to High-salt Stress through ABA-independent Pathway in Transgenic 
 Arabidopsis

Suo, Huang; Hu, Li-Qin; Xu, Dongbei; Li, Weiwei; ZhaoShi, Xu; Li, Lian-Cheng; Zhou, Yongbin; Diao, Xianmin; Jia, Guanqing; Ma, Yupo; Chen, Ming

doi:10.3724/sp.j.1006.2016.01787

Cited by 8 publications

(4 citation statements)

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“…NF-Y is a heterotrimeric transcription factor that includes three subunits: NF-YA, NF-YB, and NF-YC, and is conserved in nearly all eukaryotes. In the past two decades, many genes encoding NF-YC proteins have been identified in several species, including 20 NF-YC genes in Solanum lycopersicum [ 27 ], 15 NF-YC genes in Glycine max [ 28 ], 14 NF-YC genes Triticum aestivum [ 29 ], 14 NF-YC genes in Sorghum bicolor (L.) Moench [ 30 ], 14 NF-YC genes in Populus tomentosa [ 31 ], 13 NF-YC genes Arabidopsis thaliana [ 32 ], 13 NF-YC genes in Setaria italica [ 33 ], 12 NF-YC genes in Brachypodium distachyon [ 34 ], 12 NF-YC genes in Physcomitrella patens [ 35 ], 10 NF-YC genes in Malus Domestica [ 36 ], 9 NF-YC genes in Arachis Hypogaea L. [ 37 ], 9 NF-YC genes in Vitis vinifera [ 38 ], 7 NF-YC genes in Phaseolus vulgaris [ 39 ], 7 NF-YC genes in Z. jujube [ 40 ], 7 NF-YC genes in Oryza sativa [ 41 ], 6 NF-YC genes in Prunus persica [ 42 ], 5 NF-YC genes in Citrus [ 43 ], 4 NF-YC genes in Citrullus lanatus [ 44 ], 4 NF-YC genes in Petunia hybrida [ 45 ], and 3 NF-YC genes in Brassica napus [ 46 ]. In this study, five NF-YC coding genes were obtained by PCR amplification in wild chrysanthemums, which was less than that reported in most plants.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Characterization of Chrysanthemum indicum Nuclear Factor Y, Subunit C Gene Family Reveals the Roles of CiNF-YCs in Flowering Regulation

Wang

Yao

Wen

et al. 2022

IJMS

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Nuclear Factor Y, Subunit C (NF-YC) transcription factors are conserved in most plants, and play essential roles in plant growth and development, especially in flowering regulation. Chrysanthemums are important commercial plants, and their market value is strongly impacted by flowering time. Until now, no details regarding the NF-YC family in the Chrysanthemum genus have been available. In this study, five NF-YC genes were cloned from Chrysanthemum indicum. Multiple alignments showed that CiNF-YCs had the highly conserved characteristic regions. Phylogenetic analyses identified a pair of paralogue NF-YC proteins in chrysanthemums. Gene structure and conserved motifs were also analyzed for functional understanding. According to the results of the expression experiments, CiNF-YC1 and CiNF-YC5 were mainly expressed in leaves or flowers, and their expression levels varied greatly from the seedling to flower bud differentiation stage. Arabidopsis overexpressing CiNF-YC1 and CiNF-YC3 showed significantly delayed flowering, accompanied by other morphological alterations. RT-qPCR analysis revealed that genes associated with photoperiod, vernalization, aging, and gibberellin pathways were downregulated in CiNF-YC1-OX lines, relative to the wild type, whereas in CiNF-YC3-OX lines, only SHORT VEGETATIVE PHASE (AtSVP), the key factor in the ambient temperature pathway, was upregulated. Taken together, these findings suggest that CiNF-YC1 and CiNF-YC3 negatively regulate flowering in Arabidopsis via different flowering pathways.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Characterization of Chrysanthemum indicum Nuclear Factor Y, Subunit C Gene Family Reveals the Roles of CiNF-YCs in Flowering Regulation

Wang

Yao

Wen

et al. 2022

IJMS

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“…Nuclear factor Y (NF-Y) transcript factors have been reported to be involved in many processes, such as flowering time [ 3 , 4 , 5 , 6 , 7 ], chloroplast biogenesis [ 8 ], root growth [ 9 ], and stress response [ 10 , 11 , 12 , 13 , 14 , 15 , 16 ]. NF-Y is a heterotrimeric transcription factor that specifically binds to the CCAAT box in the promoter regions of many genes in all eukaryotes [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Analyses of Four PhNF-YC Genes from Petunia hybrida and Impacts on Flowering Time

Bin,

Tan,

Wen

et al. 2024

Plants

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Nuclear Factor Y (NF-Y) is a class of heterotrimeric transcription factors composed of three subunits: NF-A, NF-YB, and NF-YC. NF-YC family members play crucial roles in various developmental processes, particularly in the regulation of flowering time. However, their functions in petunia remain poorly understood. In this study, we isolated four PhNF-YC genes from petunia and confirmed their subcellular localization in both the nucleus and cytoplasm. We analyzed the transcript abundance of all four PhNF-YC genes and found that PhNF-YC2 and PhNF-YC4 were highly expressed in apical buds and leaves, with their transcript levels decreasing before flower bud differentiation. Silencing PhNF-YC2 using VIGS resulted in a delayed flowering time and reduced chlorophyll content, while PhNF-YC4-silenced plants only exhibited a delayed flowering time. Furthermore, we detected the transcript abundance of flowering-related genes involved in different signaling pathways and found that PhCO, PhGI, PhFBP21, PhGA20ox4, and PhSPL9b were regulated by both PhNF-YC2 and PhNF-YC4. Additionally, the transcript abundance of PhSPL2, PhSPL3, and PhSPL4 increased only in PhNF-YC2-silenced plants. Overall, these results provide evidence that PhNF-YC2 and PhNF-YC4 negatively regulate flowering time in petunia by modulating a series of flowering-related genes.

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“…In a previous study [ 25 ], 116 candidate genes were selected by mapping photoperiod-sensitive traits in millet, among which 4 were members of the NF-Y gene family. Studies on the functions of NF-Y family genes are limited to stress resistance [ 26 , 27 ], and little attention has been paid to the regulation of heading and flowering times in millet.…”

Section: Introductionmentioning

confidence: 99%

SiNF-YC2 Regulates Early Maturity and Salt Tolerance in Setaria italica

Niu

Guan

et al. 2023

IJMS

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Early maturity is an important agronomic trait in most crops, because it can solve the problem of planting in stubble for multiple cropping as well as make full use of light and temperature resources in alpine regions, thereby avoiding damage from low temperatures in the early growth period and early frost damage in the late growth period to improve crop yield and quality. The expression of genes that determine flowering affects flowering time, which directly affects crop maturity and indirectly affects crop yield and quality. Therefore, it is important to analyze the regulatory network of flowering for the cultivation of early-maturing varieties. Foxtail millet (Setaria italica) is a reserve crop for future extreme weather and is also a model crop for functional gene research in C4 crops. However, there are few reports on the molecular mechanism regulating flowering in foxtail millet. A putative candidate gene, SiNF-YC2, was isolated based on quantitative trait loci (QTL) mapping analysis. Bioinformatics analysis showed that SiNF-YC2 has a conserved HAP5 domain, which indicates that it is a member of the NF-YC transcription factor family. The promoter of SiNF-YC2 contains light-response-, hormone-, and stress-resistance-related elements. The expression of SiNF-YC2 was sensitive to the photoperiod and was related to the regulation of biological rhythm. Expression also varied in different tissues and in response to drought and salt stress. In a yeast two-hybrid assay, SiNF-YC2 interacted with SiCO in the nucleus. Functional analysis suggested that SiNF-YC2 promotes flowering and improves resistance to salt stress.

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Transcription Factor SiNF-YA5 from Foxtail Millet ( Setaria italica ) Conferred Tolerance to High-salt Stress through ABA-independent Pathway in Transgenic Arabidopsis

Cited by 8 publications

References 0 publications

Genome-Wide Characterization of Chrysanthemum indicum Nuclear Factor Y, Subunit C Gene Family Reveals the Roles of CiNF-YCs in Flowering Regulation

Genome-Wide Characterization of Chrysanthemum indicum Nuclear Factor Y, Subunit C Gene Family Reveals the Roles of CiNF-YCs in Flowering Regulation

Comprehensive Analyses of Four PhNF-YC Genes from Petunia hybrida and Impacts on Flowering Time

SiNF-YC2 Regulates Early Maturity and Salt Tolerance in Setaria italica

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