Transcription factors Atf1 and Sty1 promote stress tolerance under nitrosative stress in Schizosaccharomyces pombe

Kar, Puranjoy; Biswas, Pranjal; Patra, Sourav Kumar; Ghosh, Sudakshina

doi:10.1016/j.micres.2017.10.002

Cited by 7 publications

(8 citation statements)

References 36 publications

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“…Fungi have developed detoxification strategies to counteract the damaging effects of NO and the ROS‐responsive transcription factors also play protective important roles. For example, in S. pombe Atf1 is a key player for the initiation of the S‐phase in the cell cycle, allowing a pause in replication to respond to nitrosative stress (Kar et al ., 2018). In S. cerevisiae Yap1 is a key regulator in response to nitrosative stress by up‐regulating expression of superoxide dismutase and catalase (Lushchak et al ., 2010).…”

Section: Discussionmentioning

confidence: 99%

“…For instance, VdAtf1 is critical to an appropriate response to NO stress by regulating the transcription of genes involved in NO detoxification in V. dahliae , and an FgAtf1 mutant strain of Fusarium graminearum displayed increased sensitivity to NO (Tang et al ., 2020). In Schizosaccharomyces pombe , Atf1 accumulates in the nucleus in response to nitrosative stress to promote stress tolerance (Kar et al ., 2018), while in Saccharomyces cerevisiae , Yap1 also accumulates in the nucleus under nitrosative stress and mediates the changes in gene expression that correlate with RNS‐induced responses (Horan et al ., 2006; Lushchak et al ., 2010). Nevertheless, little is known about how Skn7 homologs mediate responses to RNS.…”

Section: Introductionmentioning

confidence: 99%

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Convergent and distinctive functions of transcription factors VdYap1, VdAtf1, and VdSkn7 in the regulation of nitrosative stress resistance, microsclerotia formation, and virulence in Verticillium dahliae

Tang

Jin

Klosterman

et al. 2020

Molecular Plant Pathology

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Vascular wilt disease caused by Verticillium dahliae impacts over 200 plant species worldwide and results in losses of agricultural production, as well as damage to ornamental plants and ecological habitats (Klosterman et al., 2009). In China, V. dahliae has not only posed a major threat to crop production, but has also taken a heavy toll on ornamental perennials like smoke trees (Cotinus coggygria), the leaves of which provide beautiful scenery at the Fragrant Hills Park in Beijing, China (Wang et al., 2013). V. dahliae penetrates plants through the roots via infectious hyphae that germinate from the long-lived soilborne microsclerotia (Fradin and Thomma, 2006; Klosterman et al., 2009). After penetration, the fungus continues to proliferate in the xylem, resulting in characteristic plant wilt symptoms (Beckman et al., 1976).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Convergent and distinctive functions of transcription factors VdYap1, VdAtf1, and VdSkn7 in the regulation of nitrosative stress resistance, microsclerotia formation, and virulence in Verticillium dahliae

Tang

Jin

Klosterman

et al. 2020

Molecular Plant Pathology

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“…Results have shown that AtfA/Atf1 and its orthologs are quite highly conserved and that they are involved in multiple cellular processes. In S. pombe, Atf1 regulates the transcription of genes involved in recombination at some hot spots and in the initiation of the mating and meiosis processes, survival at stationary phase (20), heterochromatin assembly, and responses to osmotic, heat, starvation, and nitrative stress (19,21,22). In filamentous fungi, AtfA/Atf1 regulates the expression of genes related to conidial stress in Aspergillus fumigatus, Aspergillus nidulans, and Aspergillus oryzae (23)(24)(25)(26), genes related to vegetative hyphae and/or secondary metabolism in A. nidulans (27,28), Botrytis cinerea (29), Claviceps purpurea (30), Fusarium graminearum (31), Fusarium oxysporum (32), and Magnaporthe oryzae (33), and fungal virulence in B. cinerea, C. purpurea, F. graminearum, and M. oryzae (29,30,32,33).…”

Section: Discussionmentioning

confidence: 99%

Transcription Factor Atf1 Regulates Expression of Cellulase and Xylanase Genes during Solid-State Fermentation of Ascomycetes

Zhao

Liao

Wang

et al. 2019

Appl Environ Microbiol

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Transcriptional regulation of cellulolytic and xylolytic genes in ascomycete fungi is controlled by specific carbon sources in different external environments. Here, comparative transcriptomic analyses of Penicillium oxalicum grown on wheat bran (WB), WB plus rice straw (WR), or WB plus Avicel (WA) as the sole carbon source under solid-state fermentation (SSF) revealed that most of the differentially expressed genes (DEGs) were involved in metabolism, specifically, carbohydrate metabolism. Of the DEGs, the basic core carbohydrate-active enzyme-encoding genes which responded to the plant biomass resources were identified in P. oxalicum, and their transcriptional levels changed to various extents depending on the different carbon sources. Moreover, this study found that three deletion mutants of genes encoding putative transcription factors showed significant alterations in filter paper cellulase production compared with that of a parental P. oxalicum strain with a deletion of Ku70 (ΔPoxKu70 strain) when grown on WR under SSF. Importantly, the ΔPoxAtf1 mutant (with a deletion of P. oxalicum Atf1, also called POX03016) displayed 46.1 to 183.2% more cellulase and xylanase production than a ΔPoxKu70 mutant after 2 days of growth on WR. RNA sequencing and quantitative reverse transcription-PCR revealed that PoxAtf1 dynamically regulated the expression of major cellulase and xylanase genes under SSF. PoxAtf1 bound to the promoter regions of the key cellulase and xylanase genes in vitro. This study provides novel insights into the regulatory mechanism of fungal cellulase and xylanase gene expression under SSF. IMPORTANCE The transition to a more environmentally friendly economy encourages studies involving the high-value-added utilization of lignocellulosic biomass. Solid-state fermentation (SSF), that simulates the natural habitat of soil microorganisms, is used for a variety of applications such as biomass biorefinery. Prior to the current study, our understanding of genome-wide gene expression and of the regulation of gene expression of lignocellulose-degrading enzymes in ascomycete fungi during SSF was limited. Here, we employed RNA sequencing and genetic analyses to investigate transcriptomes of Penicillium oxalicum strain EU2101 cultured on medium containing different carbon sources and to identify and characterize transcription factors for regulating the expression of cellulase and xylanase genes during SSF. The results generated will provide novel insights into genetic engineering of filamentous fungi to further increase enzyme production.

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“…Previously we have shown that Atf1 is involved in regulating the cell cycle checkpoint activation in S. pombe upon nitrosative stress [25]. To decipher the function of Atf1 and Pcr1 in S. pombe upon nitrosative stress, in the present study we rst time show that phosphorylation of Atf1 is not essential for replication checkpoint initiation, Atf1 and Pcr1 can function independently and play an antagonistic role in cell growth regulation.…”

Section: Introductionmentioning

confidence: 50%

Complex regulatory role(s) of Atf1 and Pcr1 under nitrosative stress in Schizosaccharomyces pombe

Dey,

Samaddar,

Ghosh

2023

Preprint

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Excess nitric oxide (NO) and reactive nitrogen species produce nitrosative stress in cells. Schizosaccharomyces pombe cells showed intra S-phase check point activation under nitrosative stress. In this study we investigated the role of bZIP transcription factors Atf1 and Pcr1 under nitrosative stress in S. pombe. Δatf1 and Δpcr1 strain of S. pombe showed differential growth sensitivity towards NO donor. Δatf1 strain of S. pombe showed growth acceleration whereas Δpcr1 showed growth retardation in response to NO donor. Mitotic index and flow cytometry data also showed faster mitotic entry and absence of intra S-phase checkpoint activation in Δatf1 strain of S. pombe compared to Δpcr1 strains in response to NO donor. Interestingly, phospho mutant of Atf1 also showed similar effects like the wild type indicating phosphorylation of Atf1 was not essential for inducing nitrosative stress response in S. pombe. Our previous experimental evidences established the fact that Wee1 phosphorylates Cdc2 Tyr15 which leads to replication slowdown in the fission yeast under nitrosative stress. Cdc13 is a B-type cyclin and it forms a complex with Cdc2, and this complex formation is essential for activating Cdc2. To check whether Pcr1 and Cdc13 are involved or not in Δatf1 strain of S. pombe for activating Cdc2 under nitrosative stress, we checked the transcripts of both. Both Pcr1 and Cdc13 expression were significantly increased in S. pombe upon nitrosative stress in the absence of Atf1. From our study it is evident that Atf1 and Pcr1 play complex regulatory role in S. pombe under nitrosative stress.

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Transcription factors Atf1 and Sty1 promote stress tolerance under nitrosative stress in Schizosaccharomyces pombe

Cited by 7 publications

References 36 publications

Convergent and distinctive functions of transcription factors VdYap1, VdAtf1, and VdSkn7 in the regulation of nitrosative stress resistance, microsclerotia formation, and virulence in Verticillium dahliae

Convergent and distinctive functions of transcription factors VdYap1, VdAtf1, and VdSkn7 in the regulation of nitrosative stress resistance, microsclerotia formation, and virulence in Verticillium dahliae

Transcription Factor Atf1 Regulates Expression of Cellulase and Xylanase Genes during Solid-State Fermentation of Ascomycetes

Complex regulatory role(s) of Atf1 and Pcr1 under nitrosative stress in Schizosaccharomyces pombe

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