Transcription factors ERα and Sox2 have differing multiphasic DNA and RNA binding mechanisms
Wayne O. Hemphill,
Halley R. Steiner,
Jackson R. Kominsky
et al.
Abstract:Many transcription factors (TFs) have been shown to bind RNA, leading to open questions regarding the mechanism(s) of this RNA binding and its role in regulating TF activities. Here we use biophysical assays to interrogate the kon, koff, and Kd for DNA and RNA binding of two model human transcription factors, ERα and Sox2. Unexpectedly, we found that both proteins exhibited multiphasic nucleic acid binding kinetics. We propose that Sox2 RNA and DNA multiphasic binding kinetics could be explained by a conventio… Show more
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