2024
DOI: 10.1101/2024.03.18.585577
|View full text |Cite
Preprint
|
Sign up to set email alerts
|

Transcription factors ERα and Sox2 have differing multiphasic DNA and RNA binding mechanisms

Wayne O. Hemphill,
Halley R. Steiner,
Jackson R. Kominsky
et al.

Abstract: Many transcription factors (TFs) have been shown to bind RNA, leading to open questions regarding the mechanism(s) of this RNA binding and its role in regulating TF activities. Here we use biophysical assays to interrogate the kon, koff, and Kd for DNA and RNA binding of two model human transcription factors, ERα and Sox2. Unexpectedly, we found that both proteins exhibited multiphasic nucleic acid binding kinetics. We propose that Sox2 RNA and DNA multiphasic binding kinetics could be explained by a conventio… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

0
0
0

Publication Types

Select...

Relationship

0
0

Authors

Journals

citations
Cited by 0 publications
references
References 59 publications
0
0
0
Order By: Relevance

No citations

Set email alert for when this publication receives citations?