Transcription Modulation by a Rat Nuclear Scaffold Protein, P130, and a Rat Highly Repetitive DNA Component or Various Types of Animal and Plant Matrix or Scaffold Attachment Regions

Hibino, Yasuhide; Ohzeki, Hiromitsu; Sugano, Nobuhiko; Hiraga, Kenji

doi:10.1006/bbrc.2000.3938

Cited by 38 publications

(37 citation statements)

References 19 publications

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“…These properties can be considered as the molecular basis of the diverse functions of matrin 3. We also reported that Xmn I or several MAR/SAR fragments whose base unpairing appeared to play principal roles in binding to matrin 3, when tethered upstream from the SV40 promoter increased reporter luciferase gene transcription [7].…”

Section: Discussionmentioning

confidence: 79%

“…Promoter vector pGL3 (Promega Corp, Madison, WI, USA) was used to determine the extent of augmentation of SV40 promotermediated luciferase gene transcription by the Xmn I fragment tethered up-stream from the tract encompassing the SV40 promoter and the firefly luciferase gene [7].…”

Section: Cell Linementioning

confidence: 99%

“…The extent of augmentation of transcription was determined as luciferase activity exhibited by cells that received wild type pGL3 promoter vector and pRL-SV40 [7].…”

Section: Determination Of the Extent Of Augmentation Of Transcriptionmentioning

confidence: 99%

“…It bound to a highly repetitive DNA component, an Xmn I fragment, which was also cloned from DNA contained in rat liver nuclear scaffold [5]. In addition to binding to the Xmn I fragment, matrin 3 bound to various MAR/SAR segments by recognizing an ATATAT sequence, which causes base-unpairing [6,7], as a binding site. Functionally, transient expression of reporter luciferase gene constructs, in which Xmn I or distinct MAR/SAR fragments were tethered upstream from the SV40 promoter of the pGL3 promoter vector, increased luciferase gene transcription in various types of recipient cells [7].…”

Section: Introductionmentioning

confidence: 99%

“…In addition to binding to the Xmn I fragment, matrin 3 bound to various MAR/SAR segments by recognizing an ATATAT sequence, which causes base-unpairing [6,7], as a binding site. Functionally, transient expression of reporter luciferase gene constructs, in which Xmn I or distinct MAR/SAR fragments were tethered upstream from the SV40 promoter of the pGL3 promoter vector, increased luciferase gene transcription in various types of recipient cells [7]. Bent and AT-rich regions of Xmn I and MAR/SAR fragments appeared to be required for this increased transcription.…”

Section: Introductionmentioning

confidence: 99%

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Matrin 3 Augments the Transcriptional Activity of an SV40 Promoter-Mediated Luciferase Gene with a Highly Repetitive DNA Component

Kamiuchi¹,

Fukaya²,

Usui³

et al. 2014

J Mol Genet Med

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We examined the transcriptional augmentation of matrin 3, a nuclear matrix protein, of the SV40 promotermediated luciferase gene (pGL3) following transient transfection of recombinant plasmids into cells. It has been reported that the interaction of the Xmn I fragment, a highly repetitive DNA component as one of a typical matrix-or scaffold-attachment regions (MAR/SAR) tethered upstream from the SV40 promoter (pGL3-Xmn I) with matrin 3 appeared to be required for augmentation of luciferase gene transcription. In this study, we investigated the levels of induction in cells overexpressing the wild type and several deletion mutants of matrin 3. It appeared that pGL3-Xmn I augmented luciferase production to 4-times the control level in Ac2F cells, but 23-fold in cells overexpressing matrin 3. Electrophoretic mobility shift assay showed that the Xmn I fragment augmented luciferase gene transcription through interaction with matrin 3. Furthermore, our findings suggest that all of the functional domains tested in matrin 3 were necessary for transcriptional augmentation. We aim not only to describe the transcriptional augmentation of matrin 3 with MAR/SAR, but also to strengthen interest in their use to mediate the expression of therapeutic transgenes.

show abstract

Section: Discussionmentioning

confidence: 79%

Section: Cell Linementioning

confidence: 99%

“…The extent of augmentation of transcription was determined as luciferase activity exhibited by cells that received wild type pGL3 promoter vector and pRL-SV40 [7].…”

Section: Determination Of the Extent Of Augmentation Of Transcriptionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Matrin 3 Augments the Transcriptional Activity of an SV40 Promoter-Mediated Luciferase Gene with a Highly Repetitive DNA Component

Kamiuchi¹,

Fukaya²,

Usui³

et al. 2014

J Mol Genet Med

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Knockdown of genes involved in axonal transport enhances the toxicity of human neuromuscular disease‐linked MATR3 mutations in Drosophila

et al. 2020

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Mutations in the nuclear matrix protein Matrin 3 (MATR3) have been identified in amyotrophic lateral sclerosis and myopathy. To investigate the mechanisms underlying MATR3 mutations in neuromuscular diseases and efficiently screen for modifiers of MATR3 toxicity, we generated transgenic MATR3 flies. Our findings indicate that expression of wild‐type or mutant MATR3 in motor neurons reduces climbing ability and lifespan of flies, while their expression in indirect flight muscles (IFM) results in abnormal wing positioning and muscle degeneration. In both motor neurons and IFM, mutant MATR3 expression results in more severe phenotypes than wild‐type MATR3, demonstrating that the disease‐linked mutations confer pathogenicity. We conducted a targeted candidate screen for modifiers of the MATR3 abnormal wing phenotype and identified multiple enhancers involved in axonal transport. Knockdown of these genes enhanced protein levels and insolubility of mutant MATR3. These results suggest that accumulation of mutant MATR3 contributes to toxicity and implicate axonal transport dysfunction in disease pathogenesis.

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Chromosome territories and the global regulation of the genome

Fritz

Sehgal

Pliss

et al. 2019

Genes Chromosomes & Cancer

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Spatial positioning is a fundamental principle governing nuclear processes. Chromatin is organized as a hierarchy from nucleosomes to Mbp chromatin domains (CD) or topologically associating domains (TADs) to higher level compartments culminating in chromosome territories (CT). Microscopic and sequencing techniques have substantiated chromatin organization as a critical factor regulating gene expression. For example, enhancers loop back to interact with their target genes almost exclusively within TADs, distally located coregulated genes reposition into common transcription factories upon activation, and Mbp CDs exhibit dynamic motion and configurational changes in vivo. A longstanding question in the nucleus field is whether an interactive nuclear matrix provides a direct link between structure and function. The findings of nonrandom radial positioning of CT within the nucleus suggest the possibility of preferential interaction patterns among populations of CT. Sequential labeling up to 10 CT followed by application of computer imaging and geometric graph mining algorithms revealed cell‐type specific interchromosomal networks (ICN) of CT that are altered during the cell cycle, differentiation, and cancer progression. It is proposed that the ICN correlate with the global level of genome regulation. These approaches also demonstrated that the large scale 3‐D topology of CT is specific for each CT. The cell‐type specific proximity of certain chromosomal regions in normal cells may explain the propensity of distinct translocations in cancer subtypes. Understanding how genes are dysregulated upon disruption of the normal “wiring” of the nucleus by translocations, deletions, and amplifications that are hallmarks of cancer, should enable more targeted therapeutic strategies.

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Transcription Modulation by a Rat Nuclear Scaffold Protein, P130, and a Rat Highly Repetitive DNA Component or Various Types of Animal and Plant Matrix or Scaffold Attachment Regions

Cited by 38 publications

References 19 publications

Matrin 3 Augments the Transcriptional Activity of an SV40 Promoter-Mediated Luciferase Gene with a Highly Repetitive DNA Component

Matrin 3 Augments the Transcriptional Activity of an SV40 Promoter-Mediated Luciferase Gene with a Highly Repetitive DNA Component

Knockdown of genes involved in axonal transport enhances the toxicity of human neuromuscular disease‐linked MATR3 mutations in Drosophila

Chromosome territories and the global regulation of the genome

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