Transcription of feline calicivirus RNA

Abstract

We report here the cloning and 3' sequence determination of feline calicivirus strain F9. Subcloning the 3' terminus enabled the production of strand specific probes for RNA synthesis. We extend the number of virus specific RNAs detected intracellularly to 8, and show that numbers 1-5 are represented as negative strands which may serve as templates in the synthesis of these RNAs.

Detection of the ORF3 polypeptide of feline calicivirus in infected cells and evidence for its expression from a single, functionally bicistronic, subgenomic mRNA

“…The predicted protein shows 90-94 % identity at the amino acid sequence level amongst five different FCV strains (Seal et al, 1993). A homologous small ORF is also present at the 3" end of NV, RHDV, VESV and SMSV (Carter, 1990;Meyers et al, t991;Neill, 1992;Jiang et al, 1993) and is conserved at the amino acid sequence level within members (Milton et al, 1992;Neill, 1992;Seal et al, 1993). The conservation of this hypothetical polypeptide argues that it plays a functional role.…”

“…The template RNA for the production of ORF3 is uncertain. It has been suggested that a shorter subgenomic RNA of 0.5 kb may be present in FCV-infected cells (Carter, 1990) which could serve as the template. However, when oligo(dT)-selected FCV RNA was translated in RRL and the products immunoprecipitated with anti-ORF3 antiserum (Fig.…”

“…In addition to genomic RNA, Northern blot analysis has identified a number of additional mRNAs in different studies. These include 3'-co-terminal RNAs of 4.8, 4.2 and 2.4kb (Neill & Mengeling, 1988) and 5-3, 4.3, 3'6, 2.7, 1-9, 1.5 and 0.55 kb (Carter, 1990). Double-stranded forms of the genomic RNA and the 2-4 kb message (Neill & Mengeling, 1988;Neill et al, 1991) and negative strands corresponding to the genomic and 5.3, 4.3, 3-6 and 2.7 kb subgenomic RNAs were also detected (Carter, 1990).…”

“…These include 3'-co-terminal RNAs of 4.8, 4.2 and 2.4kb (Neill & Mengeling, 1988) and 5-3, 4.3, 3'6, 2.7, 1-9, 1.5 and 0.55 kb (Carter, 1990). Double-stranded forms of the genomic RNA and the 2-4 kb message (Neill & Mengeling, 1988;Neill et al, 1991) and negative strands corresponding to the genomic and 5.3, 4.3, 3-6 and 2.7 kb subgenomic RNAs were also detected (Carter, 1990). Thus there are considerable differences between the number of RNA species detected in FCV-infected cells and those found in cells infected by a number of other caliciviruses.…”

Analysis of feline calicivirus capsid protein genes: identification of variable antigenic determinant regions of the protein

“…The most abundant transcript is a 2.4 kb subgenomic RNA which can be detected as early as 2 h p.i. (Neill & Mengeling, 1988;Carter, 1990) and has been identified as the transcript encoding the viral capsid protein (Neill et al, 1991). The capsid protein gene has been sequenced for the CFI/68 isolate (Neill et al, 1991), the F4 isolate (Toya et al, 1991b) and F9 isolate (Carter et al, 1992a, b) of FCV.…”