Transcription of ftsZ oscillates during the cell cycle of Escherichia coli.

Garrido, Teresa; Sánchez, M. Calderón De La Barca; Palacios, Pilar; Aldea, Martí; Vicente, Miguel

doi:10.1002/j.1460-2075.1993.tb06073.x

Cited by 116 publications

(138 citation statements)

References 32 publications

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“…The data corroborate previous results showing that changes in transcript levels are correlated with the C period (Zhou et al, 1997). Our results are also consistent with the finding that transcription of ftsZ, a gene involved in cell division, is correlated with the replication cycle, and in particular replication initiation (Garrido et al, 1993;Zhou and Helmstetter, 1994).…”

Section: Changes In Transcript Levels Are Related To the C Periodsupporting

confidence: 93%

Cell cycle progression in Escherichia coli B/r affects transcription of certain genes: Implications for synthetic genome design

Echtenkamp

Wilson

Shuler

2008

Biotech & Bioengineering

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We propose that transcript levels for some genes are affected by the bacterial cell division cycle and this may be an important factor to consider when designing synthetic bacterial genomes. To test this hypothesis, transcript levels of 58 genes in Escherichia coli B/r A were determined at five times during the cell division cycle. A two-step ANOVA technique was used to analyze data from custom oligonucleotide microarrays containing genes involved in important cellular processes including central metabolism, macromolecular synthesis, and transport and secretion. Consistent with results previously found in Caulobacter, approximately 17% of the transcript levels were cell cycle dependent. Cell cycle regulation can be divided into two classes: genes displaying increased transcript concentrations following gene replication and genes displaying an increased transcript concentration prior to replication initiation. Transcripts levels for hns, uspA, and zwf were affected by the cell division cycle, but did not fit well into either class. These results indicate that transcription of a significant fraction of the genome is affected by replication cycle progression. The results also show that both physical gene position and the physiological function of a gene affect when it is transcribed. In addition to the simple association with replication fork progression, other phenomena must be occurring to account for some of our observations. In conclusion, gene position, with regard to the C period, and gene function are important factors to incorporate into design criteria for synthetic bacterial genomes.

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Section: Changes In Transcript Levels Are Related To the C Periodsupporting

confidence: 93%

Cell cycle progression in Escherichia coli B/r affects transcription of certain genes: Implications for synthetic genome design

Echtenkamp

Wilson

Shuler

2008

Biotech & Bioengineering

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“…Total RNA was prepared from 10 ml cultures as described previously (Garrido et al, 1993). For primer extension studies, RNA was used as template for the synthesis of cDNA by AMV reverse transcriptase (Boehringer Mannheim).…”

Section: Methodsmentioning

confidence: 99%

The genes for erythritol catabolism are organized as an inducible operon in Brucella abortus The GenBank accession number for the sequence reported in this paper is U57100.

2000

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Erythritol utilization is a characteristic of pathogenic Brucella abortus strains. The attenuated vaccine strain B19 is the only Brucella strain that is inhibited by erythritol, so a role for erythritol metabolism in virulence is suspected. A chromosomal fragment from the pathogenic strain B. abortus 2308 containing genes for the utilization of erythritol was cloned taking advantage of an erythritol-sensitive Tn5 insertion mutant. The nucleotide sequence of the complete 7714 bp fragment was determined. Four ORFs were identified in the sequence. The four genes were closely spaced, suggesting that they were organized as a single operon (the ery operon). The first gene (eryA) encoded a 519 aa putative erythritol kinase. The second gene (eryB) encoded an erythritol phosphate dehydrogenase. The function of the third gene (eryC) product was tentatively assigned as D-erythrulose-1-phosphate dehydrogenase and the fourth gene (eryD) encoded a regulator of ery operon expression. The operon promoter was located 5' to eryA, and contained an IHF (integration host factor) binding site. Transcription from this promoter was repressed by EryD, and stimulated by erythritol. Functional IHF was required for expression of the operon in Escherichia coli, suggesting a role for IHF in its regulation in B. abortus. The results obtained will be helpful in clarifying the role of erythritol metabolism in the virulence of Brucella spp.

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“…There are several putative DnaA boxes in the ftsQAZ operons (Robinson et al, 1984), but DnaA control of fts expression has been controversial and different experimental setups have given different results (Garrido et al, 1993;Masters et al, 1989;Smith et al, 1996). To investigate whether deletion or the presence of extra copies of the datA site affected the transcription of genes involved in cell division, we compared gene expression in exponentially growing DdatA cells and cells with MiniR1-datA to that of wild-type cells by microarray analysis.…”

Section: The Inhibitory Effect Of Data Is Not Dependent On the Sos Rementioning

confidence: 99%

The Escherichia coli datA site promotes proper regulation of cell division

Flåtten

Skarstad

2014

Microbiology

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In Escherichia coli inhibition of replication leads to a block of cell division. This checkpoint mechanism ensures that no cell divides without having two complete copies of the genome to pass on to the two daughter cells. The chromosomal datA site is a 1 kb region that contains binding sites for the DnaA replication initiator protein, and which contributes to the inactivation of DnaA. An excess of datA sites provided on plasmids has been found to lead to both a delay in initiation of replication and in cell division during exponential growth. Here we have investigated the effect of datA on the cell division block that occurs upon inhibition of replication initiation in a dnaC2 mutant. We found that this checkpoint mechanism was aided by the presence of datA. In cells where datA was deleted or an excess of DnaA was provided, cell division occurred in the absence of replication and anucleate cells were formed. This finding indicates that loss of datA and/or excess of DnaA protein promote cell division. This conclusion was supported by the finding that the lethality of the division-compromised mutants ftsZ84 and ftsI23 was suppressed by deletion of datA, at the lowest non-permissive temperature. We propose that the cell division block that occurs upon inhibition of DNA replication is, at least in part, due to a drop in the concentration of the ATP-DnaA protein. INTRODUCTIONIn Escherichia coli cell division involves the formation of a septum that separates the cell into two daughter cells. It is crucial that the two daughter nucleoids are segregated into each half of the cell, so that each daughter cell receives a copy of the genetic material and that none of the nucleoids are trapped in the septum. To ensure this, cell division and chromosome segregation are highly regulated processes involving a number of different proteins (see Egan & Vollmer, 2013;Harry et al., 2006;and Weiss, 2004, for reviews), the most studied being FtsZ. This protein polymerizes to form a ring-like structure at the site of division and serves as a scaffold for recruitment of other division proteins (Bi & Lutkenhaus, 1991). How the timing of the formation of the FtsZ ring is regulated is not clear.It is known that the chromosome replication cycle is independent of the cell division cycle, but that cell division is dependent on chromosome replication (Boye & Nordström, 2003; Nordström et al., 1991). That is, a blockage of chromosome replication stops cell division (Helmstetter & Pierucci, 1968;Walker & Pardee, 1968). Thus, a checkpoint mechanism must exist. The mechanism ensures that cell division does not occur in the absence of replication and thus prevents the formation of DNA-free (anucleate) daughter cells (Boye & Nordström, 2003; Nordström et al., 1991). Whether this mechanism is related to other regulatory circuits necessary for coupling the division cycle to cell physiology and to the growth rate of the cells, is not clear (see Haeusser & Levin, 2008, for a review).The DnaA protein is one of the main players in initiation of replica...

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Transcription of ftsZ oscillates during the cell cycle of Escherichia coli.

Cited by 116 publications

References 32 publications

Cell cycle progression in Escherichia coli B/r affects transcription of certain genes: Implications for synthetic genome design

Cell cycle progression in Escherichia coli B/r affects transcription of certain genes: Implications for synthetic genome design

The genes for erythritol catabolism are organized as an inducible operon in Brucella abortus The GenBank accession number for the sequence reported in this paper is U57100.

The Escherichia coli datA site promotes proper regulation of cell division

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