Transcription of genes associated with nickel resistance induced by different doses of nickel nitrate in Quercus rubra

Djeukam, Charnelle L.; Paul, M.; Nkongolo, K. K.

doi:10.1080/02757540.2019.1654462

Cited by 7 publications

(2 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This increase seems to be driven by potassium rather than nitrate. This is consistent with Djeukam et al [18] who also reported that potassium nitrate triggers gene expression changes in Quercus rubra. In fact, potassium nitrate induced an upregulation of Nicotianamine synthase (NAS3) gene in Quercus rubra leaves exposed to 150 mg/kg dose and a downregulation for the 1600 mg/kg treatment [18].…”

Section: Resultssupporting

confidence: 92%

Expression of Genes Associated with Nickel Resistance in White Spruce (Picea glauca) under Nickel Stress: Analysis of AT2G16800 and NRAMP Genes

Boyd¹,

Nkongolo²

2020

AJPS

View full text Add to dashboard Cite

Heavy metals such nickel (Ni) can cause toxicity by 1) displacing essential components in the biomolecules, 2) blocking the functional group of molecules, or 3) modifying enzymes, proteins, the plasma membrane, and membrane transporters. The main objective of the present study was to investigate the effect of nickel (Ni) on gene expression of nitrate on gene expression with a focus on the genes coding for the high affinity Ni transporter family protein AT2G16800, and natural resistance-associated macrophage protein (NRAMP). Ni toxicity was assessed by treating seedlings with an aqueous solution of nickel nitrate salt [Ni(NO 3) 2 ] at the concentrations of 150 mg, 800 mg, and 1600 mg of nickel per 1 kg of dry soil. RT-qPCR was used to measure the expression of AT2G16800, and NRAMP genes in samples treated with nickel nitrates and controls. The results revealed that P. glauca is resistant to Ni based on lack of plant damage at all nickel concentrations. Ni has no effect on the expression of the AT2G16800 gene in needles or roots. However, it induced an upregulation of the NRAMP genes in roots at all the doses tested (150 mg/kg, 800 mg/kg, and 1600 mg/kg). On the other hand, Ni has no effect on the expression of the NRAMP gene in needle but the lowest dose of potassium (150 mg/kg) upregulated this gene in needle tissues.

show abstract

Section: Resultssupporting

confidence: 92%

Expression of Genes Associated with Nickel Resistance in White Spruce (Picea glauca) under Nickel Stress: Analysis of AT2G16800 and NRAMP Genes

Boyd¹,

Nkongolo²

2020

AJPS

View full text Add to dashboard Cite

show abstract

“…A gene expression study on northern red oak ( Quercus rubra) conducted by Djeukam et al . [ 54 ] found that other genes associated with metal-stress including ACC, SAT, and NAS3 could be upregulated in response to high doses of nickel nitrate (800 mg/kg and 1,600 mg/kg). The red oak is another important species for the GSR because it is classified as a Ni/Zn accumulator and has the ability to store these metals in its leaves at high concentrations [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of trembling aspen (Populus tremuloides) under nickel stress

Czajka

Nkongolo²

2022

PLoS ONE

View full text Add to dashboard Cite

Plants have evolved heavy metal tolerance mechanisms to adapt and cope with nickel (Ni) toxicity. Decrypting whole gene expression of Trembling Aspen (Pinus tremuloides) under nickel stress could elucidate the nickel resistance/tolerance mechanisms. The main objectives of the present research were to 1) characterize the P. tremuloides transcriptome, and 2) compare gene expression dynamics between nickel-resistant and nickel-susceptible P. tremuloides genotypes with Whole Transcriptome (WT) sequencing. Illumina Sequencing generated 27–45 million 2X150 paired-end reads of raw data per sample. The alignment performed with StringTie Software added two groups of transcripts to the draft genome annotation. One group contained 32,677 new isoforms that match to 17,254 genes. The second group contained 17,349 novel transcripts that represent 16,157 novel genes. Overall, 52,987 genes were identified from which 36,770 genes were selected as differently expressed. With the high stringency (two-fold change, FDR value ≤ 0.05 and logFC value ≥1 (upregulated) or ≤ -1 (downregulated), after GSEA analysis and filtering for gene set size, 575 gene sets were upregulated and 146 were downregulated in nickel resistant phenotypes compared to susceptible genotypes. For biological process, genes associated with translation were significantly upregulated while signal transduction and cellular protein process genes were downregulated in resistant compared to susceptible genotypes. For molecular function, there was a significant downregulation of genes associated with DNA binding in resistant compared to susceptible lines. Significant upregulation was observed in genes located in ribosome while downregulation of genes in chloroplast and mitochondrion were preponderant in resistant genotypes compared to susceptible. Hence, from a whole transcriptome level, an upregulation in ribosomal and translation activities was identified as the main response to Ni toxicity in the resistant plants. More importantly, this study revealed that a metal transport protein (Potrs038704g29436 –ATOX1-related copper transport) was among the top upregulated genes in resistant genotypes when compared to susceptible plants. Other identified upregulated genes associated with abiotic stress include genes coding for Dirigent Protein 10, GATA transcription factor, Zinc finger protein, Auxin response factor, Bidirectional sugar transporter, and thiamine thiazole synthase.

show abstract

Contrasting tolerance and gene expression between white pine (Pinus strobus) and jack pine (P. banksiana) exposed to an increasing nickel concentration

Moarefi

Nkongolo

2022

Ecological Genetics and Genomics

View full text Add to dashboard Cite

Transcription of genes associated with nickel resistance induced by different doses of nickel nitrate in Quercus rubra

Cited by 7 publications

References 44 publications

Expression of Genes Associated with Nickel Resistance in White Spruce (<i>Picea glauca</i>) under Nickel Stress: Analysis of AT2G16800 and <i>NRAMP</i> Genes

Expression of Genes Associated with Nickel Resistance in White Spruce (<i>Picea glauca</i>) under Nickel Stress: Analysis of AT2G16800 and <i>NRAMP</i> Genes

Transcriptome analysis of trembling aspen (Populus tremuloides) under nickel stress

Contrasting tolerance and gene expression between white pine (Pinus strobus) and jack pine (P. banksiana) exposed to an increasing nickel concentration

Contact Info

Product

Resources

About

Transcription of genes associated with nickel resistance induced by different doses of nickel nitrate in Quercus rubra

Cited by 7 publications

References 44 publications

Expression of Genes Associated with Nickel Resistance in White Spruce (&lt;i&gt;Picea glauca&lt;/i&gt;) under Nickel Stress: Analysis of AT2G16800 and &lt;i&gt;NRAMP&lt;/i&gt; Genes

Expression of Genes Associated with Nickel Resistance in White Spruce (&lt;i&gt;Picea glauca&lt;/i&gt;) under Nickel Stress: Analysis of AT2G16800 and &lt;i&gt;NRAMP&lt;/i&gt; Genes

Transcriptome analysis of trembling aspen (Populus tremuloides) under nickel stress

Contrasting tolerance and gene expression between white pine (Pinus strobus) and jack pine (P. banksiana) exposed to an increasing nickel concentration

Contact Info

Product

Resources

About

Expression of Genes Associated with Nickel Resistance in White Spruce (<i>Picea glauca</i>) under Nickel Stress: Analysis of AT2G16800 and <i>NRAMP</i> Genes

Expression of Genes Associated with Nickel Resistance in White Spruce (<i>Picea glauca</i>) under Nickel Stress: Analysis of AT2G16800 and <i>NRAMP</i> Genes