Transcription of multimeric tRNA genes

Ciliberto, Gennaro; Traboni, Cinzia; Cortese, Riccardo

doi:10.1093/nar/12.2.1277

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…We (11) and others (12) have shown that, as in Drosophila systems (13), homologous in vitro transcription of yeast tRNA genes was dependent, besides the ICRs, on a 5' flanking region the sequence of which varies slightly between position -1 and -10, and can vary somewhat more upstream of position -10. This is in contrast to HoLa or Nucleic Acids Research oocyto systems, whoro any stretch of DNA can function as a 5' flank for tDNA (14). Tho transcriptional stop signal, ,4Ts, is part of the 3'flanking DNA, a rogion that in some systems appears to also influoneo the efficiency of transcription (15).…”

Section: Introductionmentioning

confidence: 94%

A yeast tRNA^Arggene can act as promoter for a 5′ flank deficient, non-transcribable tRNA^SUP6gene to produce biologically active suppressor tRNA

Stråby¹

1988

Nucl Acids Res

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In S. cerevisiae most tRNA genes are located and expressed as single entities. The tDNA(Arg)-tDNA(Asp) pair, however, is transcribed into a dimeric precursor before being processed into two mature tRNA species. The second gene of this pair, tDNA(Asp), is totally dependent on the first gene, tDNA(Arg), and its promoter components, for homologous in vitro transcription. The second gene in the pair is now replaced by the ochre suppressor tDNA(SUP)6-o, which, by itself, cannot be transcribed because of a nonfunctional 5' flanking region. The tDNA(Arg)-tDNA(SUP)6-o was transcribed into a dimeric precursor which was processed to mature tRNA molecules as judged in vitro by electrophoretic separation, and in vivo by their ability to suppress ochre but not amber yeast mutations. Mutations in the internal promoter of the first gene decreased transcription, both in vitro and in vivo, of the second-tRNA(SUP)6-o-gene. Thus tDNA(Arg) with its 5' flanking region can act as an external promoter for other RNA polymerase III-read genes that are by themselves inactive due to impaired promoter/modulator regions.

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Section: Introductionmentioning

confidence: 94%

A yeast tRNA^Arggene can act as promoter for a 5′ flank deficient, non-transcribable tRNA^SUP6gene to produce biologically active suppressor tRNA

Stråby¹

1988

Nucl Acids Res

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Characterization of transcription and processing from plasmids that use polIII and a yeast tRNA gene as promoter to transcribe promoterdeficient downstream DNA

Otter

Edqvist

Stråby

1992

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expres

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Transcription of two classes of rat growth hormone gene-associated repetitive DNA: differences in activity and effects of tandem repeat structure

et al. 1984

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The rat growth hormone (rGH) gene contains two classes of repetitive DNA arranged as clusters within intron B and the 3' flanking region. The major family is equivalent to the CHO type 2 DNA. The second ("truncated repeat", TR) is a truncated version of the first and occurs in certain neural-specific transcripts and genes ("identifier" elements, ID). Here we report, using the HeLa cell-free transcription assay, that RNA polymerase III (Pol III) efficiently initiates at internal promoters within a tandem array of rGH gene repetitive DNA monomers and results in a novel organization of overlapping Class III transcription units. Transcription competition studies revealed that the rat type 2 structures share Pol III transcription factors with a tRNA gene, a human Alu repeat, and a mutant VAl gene. Also, the rGH type 2 but not the TR DNA efficiently promotes Pol III initiation, yet other TR members, which differ only in flanking DNA, are transcribed. Thus, the rGH gene is strikingly enriched with 10 repetitive DNA monomers; multimeric type 2 elements are actively transcribed; rGH-TR sequences are expressed only as part of larger transcripts promoted by type 2 DNA; and, type 2 DNA uses tRNA gene transcription factors. These studies show that flanking sequences, promoter organization and factor competition may all affect rat repetitive DNA expression. INTRODUCTIONThe major component of mammalian short-length, middle-repetitive DNA is

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Transcription of multimeric tRNA genes

Cited by 3 publications

References 22 publications

A yeast tRNA^Arggene can act as promoter for a 5′ flank deficient, non-transcribable tRNA^SUP6gene to produce biologically active suppressor tRNA

A yeast tRNA^Arggene can act as promoter for a 5′ flank deficient, non-transcribable tRNA^SUP6gene to produce biologically active suppressor tRNA

Characterization of transcription and processing from plasmids that use polIII and a yeast tRNA gene as promoter to transcribe promoterdeficient downstream DNA

Transcription of two classes of rat growth hormone gene-associated repetitive DNA: differences in activity and effects of tandem repeat structure

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Transcription of multimeric tRNA genes

Cited by 3 publications

References 22 publications

A yeast tRNAArggene can act as promoter for a 5′ flank deficient, non-transcribable tRNASUP6gene to produce biologically active suppressor tRNA

A yeast tRNAArggene can act as promoter for a 5′ flank deficient, non-transcribable tRNASUP6gene to produce biologically active suppressor tRNA

Characterization of transcription and processing from plasmids that use polIII and a yeast tRNA gene as promoter to transcribe promoterdeficient downstream DNA

Transcription of two classes of rat growth hormone gene-associated repetitive DNA: differences in activity and effects of tandem repeat structure

Contact Info

Product

Resources

About

A yeast tRNA^Arggene can act as promoter for a 5′ flank deficient, non-transcribable tRNA^SUP6gene to produce biologically active suppressor tRNA

A yeast tRNA^Arggene can act as promoter for a 5′ flank deficient, non-transcribable tRNA^SUP6gene to produce biologically active suppressor tRNA