Transcription of Spontaneously Released Bacterial Deoxyribonucleic Acid in Frog Auricles

Stroun, Maurice; Anker, Philippe

doi:10.1128/jb.114.1.114-120.1973

Cited by 17 publications

(8 citation statements)

References 30 publications

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“…These results show that when human cells and bacteria are present with each other, as can be the case during septicemia or peritonitis, human cells can take up bacterial DNA, as we had previously observed in frogs 11,12. It has already been shown that bacterial DNA may circulate freely in the blood7,12 and that nuclear material can cross both cellular14 and nuclear membranes,15 gain access to the host genome,16 and eventually become integrated17 in mammalian cells, but this is the first time, to our knowledge, that the transcession of a distinct bacterial gene in a human cell has been reported.…”

Section: Discussionsupporting

confidence: 70%

“…Large amounts of bacterial RNA are synthesized in the cells of plants infected with bacteria, and there is evidence that spontaneously released bacterial DNA is taken up by host cells and transcribed 9,10. A similar phenomenon has been observed in frog auricles infected with bacteria,11 and we previously reported the presence of newly synthesized bacterial RNA in frog brains, even though these are naturally protected by a barrier against bacteria 12. Moreover, we have shown that newly synthesized bacterial RNA can be recovered from frog auricles after these have been in contact with a bacteria‐free supernatant.…”

Section: Introductionmentioning

confidence: 54%

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Transcession of DNA from Bacteria to Human Cells in Culture: A Possible Role in Oncogenesis

Anker¹,

Zajac²,

Lyautey³

et al. 2004

Annals of the New York Academy of Sciences

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The human organism is continuously in close contact with microorganisms, especially bacteria. In the present work, by means of a real-time polymerase chain reaction (PCR) technique, we looked for the presence of a distinct bacterial gene in human cells. To this end, we cultured a human cell line, HL60, in a supernatant in which bacteria (Bacillus subtilis) had been grown. A transient transcession of bacterial DNA into the human cells was observed.

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Section: Discussionsupporting

confidence: 70%

Section: Introductionmentioning

confidence: 54%

Transcession of DNA from Bacteria to Human Cells in Culture: A Possible Role in Oncogenesis

Anker¹,

Zajac²,

Lyautey³

et al. 2004

Annals of the New York Academy of Sciences

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“…It may have a physiological and/or pathological role. For instance, DNA has been shown to spontaneously pass from prokaryotic cells to eukaryotic cells, 36–38 where it may be transcribed. The passage of DNA from eukaryote to eukaryote cells may also occur 39–41.…”

Section: Why?mentioning

confidence: 99%

The Origin and Mechanism of Circulating DNA

Stroun

Maurice

Vasioukhin

et al. 2000

Annals of the New York Academy of Sciences

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343

218

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“…En experimentos in vivo e in vitro con células y tejidos animales, se ha demostrado que tras su exposición a bacterias, las células y tejidos animales pueden sintetizar ARN bacteriano [162][163][164][165][166] .…”

Section: Internalización Del Materials Genético Externo En El Hospederounclassified

Consecuencias de la transferencia horizontal de genes en la evolución genómica eucarionte [Consequences of horizontal gene transfer in eukaryotic genomic evolution]

Martínez‐Ezquerro¹

2020

Preprint

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Gene exchange between prokaryotes has long been recognized as an important biological process, to the extent that it has been proposed that a more suitable metaphor to describe the evolutionary process, at least in prokaryotes, should be that of "the web of life", where lines of descent not only diverge but also communicate and even fuse with each other, resulting in a reticulated pattern. This pattern partially describes and outlines the actual complexity because, with the exception of some ancestral horizontal gene transfer (HGT) events, it relegates most eukaryotes.In this work, I summarize the evidence on how such lines of descent diverge, communicate, and merge with each other, in both prokaryotes and eukaryotes, involving deep branches and recent divergences. In fact, both direct and indirect evidence shows the existence of active HGT mechanisms in eukaryotes, indicating that horizontal transfer of genetic material is an ongoing evolutionary process in these organisms, as important as it is in the case of prokaryotes. Some of the consequences in eukaryotic genomic evolution that this phenomenon implies include, as in prokaryotes, the acquisition of novel functions, reacquisition of lost genes, and incorporation of adaptive advantages.HGT involves not only phagocytic single-celled eukaryotes but all eukaryotes —protists, plants, fungi, and animals, including humans. This lateral transfer of genetic material occurs through homologous HGT mechanisms that also exist in bacteria: conjugation, transduction, endogenization, and transformation. Given the evidence, I suggest a global economy of genetic material among all current organisms: viruses, prokaryotes, and eukaryotes; fading the notion of barriers between species, as a result of the continuous horizontal circulation and use of available genetic material.Finally, I propose an ecological classification focused on the various interactions with which foreign genetic material is obtained, to facilitate the understanding of HGT as an ecological and evolutionary phenomenon, natural and global, that affects all organisms and involves all reservoirs of genetic material. **Resumen**Desde hace tiempo, se reconoce al intercambio de genes entre procariontes como un proceso biológico importante, al grado de que se ha propuesto que una metáfora más adecuada para describir el proceso evolutivo, al menos procarionte, debería ser la de “la red de la vida” en donde las líneas de la descendencia no sólo divergen sino también se comunican e incluso se fusionan unas con otras, dando como resultado un patrón reticulado. Este patrón describe y esquematiza parcialmente la complejidad real debido a que, con excepción de algunos eventos de transferencia horizontal de genes (THG) ancestrales, relega a la mayoría de los eucariontes.En este trabajo sintetizo la evidencia sobre cómo dichas líneas de descendencia divergen, se comunican y fusionan unas con otras, tanto en procariontes como en eucariontes, involucrando ramas profundas y divergencias recientes. De hecho, diversas evidencias tanto directas como indirectas, muestran la existencia de mecanismos de THG activos en los eucariontes, indicando que la transferencia horizontal de material genético es un proceso evolutivo actual en estos organismos, tan importante como lo es en el caso de los procariontes. Algunas de las consecuencias en la evolución genómica eucarionte que este fenómeno implica incluyen, al igual que en los procariontes, la adquisición de funciones novedosas, readquisición de genes perdidos e incorporación de ventajas adaptativas.La THG involucra no sólo a eucariontes unicelulares principalmente fagocíticos sino a todos los eucariontes —protistas, plantas, hongos y animales, incluyendo a los humanos. Esta transferencia lateral de material genético occure mediante mecanismos homólogos de THG que también existen en bacterias: conjugación, transducción, endogenización y transformación. Dada la evidencia, planteo una economía global del material genético entre todos los organismos actuales —virus, procariontes y eucariontes; desvaneciendo la noción de barreras entre las especies, resultado de la continua circulación horizontal y aprovechamiento del material genético disponible. Finalmente, propongo una clasificación ecológica centrada en las diversas interacciones con las que se obtiene el material genético foráneo, para facilitar el entendimiento de la THG como un fenómeno ecológico y evolutivo, natural y global, que afecta a todos los organismos e involucra a todos los reservorios de material genético.

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Transcription of Spontaneously Released Bacterial Deoxyribonucleic Acid in Frog Auricles

Cited by 17 publications

References 30 publications

Transcession of DNA from Bacteria to Human Cells in Culture: A Possible Role in Oncogenesis

Transcession of DNA from Bacteria to Human Cells in Culture: A Possible Role in Oncogenesis

The Origin and Mechanism of Circulating DNA

Consecuencias de la transferencia horizontal de genes en la evolución genómica eucarionte [Consequences of horizontal gene transfer in eukaryotic genomic evolution]

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