Transcription of the Adenovirus Genome by an α-Amanitine-Sensitive Ribonucleic Acid Polymerase in HeLa Cells

Price, Richard; Penman, Sheldon

doi:10.1128/jvi.9.4.621-626.1972

Cited by 130 publications

(26 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reason for this switch in promoter specificity is unknown. Both early and late mRNAs are tran -scribed by cellular RNA polymerase II (5,6), and no major changes seem to occur in the nature of the enzyme between early and late times to account for the switch in specificity of promoter binding. This does not rule out the possibility that there may be as yet unidentified virus-coded proteins involved in determining promoter specificity.…”

Section: Introductionmentioning

confidence: 99%

Parental adenovirus DNA accumulates in nucleosome-like structures in infected cells

Tate¹,

Philipson²

1979

Nucl Acids Res

View full text Add to dashboard Cite

Micrococcal-nuclease digestion of adenovirus 2(ad 2) infected HeLa cell nuclei early after infection has been used to investigate the nucleoprotein nature of parental viral DNA. Viral DNA is more susceptible to nuclease digestion than cellular DNA. The pattern of digestion products changes as digestion proceeds from an indistinct pattern 1 hour post infection(pi) to a nucleosome-like pattern at 6 hours pi. The major differences between viral and cellular nucleoprotein products were i) a subnucleosome fraction from viral DNA and ii) the repeat size of DNA in viral nucleosomes was 165 base pairs and in cellular nucleosomes, 195 base pairs. Up to 50% viral DNA in nuclei 6 hours pi seems to be in nucleosome-like structures. Such patterns are not seen on digestion of partially-uncoated virus or isolated cores.

show abstract

Section: Introductionmentioning

confidence: 99%

Parental adenovirus DNA accumulates in nucleosome-like structures in infected cells

Tate¹,

Philipson²

1979

Nucl Acids Res

View full text Add to dashboard Cite

show abstract

“…RNA polymerase II transcribes both strands of the viral DNA over nearly all of its length, and the resultant mRNAs encode more than 50 viral proteins. RNA polymerase III transcribes less than 1% of the viral genome, giving rise to one or two species (depending on the virus serotype) of short, noncoding RNAs (44,59). This RNA was named virus-associated (VA) RNA when its origin from the viral genome was still uncertain (45).…”

mentioning

confidence: 99%

Adenovirus virus-associated RNA and translation control

Mathews

Shenk

1991

J Virol

370

125

View full text Add to dashboard Cite

“…4) indicates that the in vitro splicing reaction is a faithful rendition of the events that occur in the intact cell. DISCUSSION It has been known for some time that form II RNA polymerase will transcribe adenoviral DNA in nuclei isolated from productively infected human cells (30,38), with from 18 to 30% of the in vitro RNA product being virus specific (30,37,38,41). Such adenoviral RNA sequences have also been described to be heterogeneous in size (30, 37) and to be transcribed from the major, r-strand transcriptional unit shown in Fig.…”

Section: Resultsmentioning

confidence: 98%

Synthesis and Processing of Adenoviral RNA in Isolated Nuclei

Yang

Flint

1979

J Virol

View full text Add to dashboard Cite

Adenoviral RNA sequences synthesized in nuclei isolated during the late phase of productive infection comprise, besides virus-associated, VA, RNA, five major species, ranging in size from approximately 13S to 55S. The latter RNA species is of the length predicted if the major transcriptional unit expressed during the late phase were completely copied in vitro. Some 30% of the RNA sequences labeled in vitro are polyadenylated, and about one-third of the polyadenylated RNA is virus specific. Hybridization analysis of the sequences immediately adjacent to polyadenylic acid in late RNA labeled in isolated nuclei suggests that polyadenylation in vitro occurs at the same sites recognized within the cell. The polyadenylic acid-containing viral RNA sequences made in isolated nuclei are found in three major species of RNA, sedimenting at approximately 28S, 18S, and 13S. These sizes are remarkably similar to those reported for late mRNA species, suggesting that additional processing steps can occur in isolated nuclei. Hybridization of RNA to XhoI fragments of adenovirus type 2 DNA transferred to nitrocellulose filters reveals that sequences complementary to the region from 22.0 to 26.5 units present in 55S RNA are absent from all smaller species, suggesting that the smaller RNA species labeled in isolated nuclei are generated by splicing. The splicing events necessary to generate the 5' leader segment common to the majority of late adenoviral mRNA species are shown to be performed correctly in isolated nuclei.

show abstract

Transcription of the Adenovirus Genome by an α-Amanitine-Sensitive Ribonucleic Acid Polymerase in HeLa Cells

Cited by 130 publications

References 21 publications

Parental adenovirus DNA accumulates in nucleosome-like structures in infected cells

Parental adenovirus DNA accumulates in nucleosome-like structures in infected cells

Adenovirus virus-associated RNA and translation control

Synthesis and Processing of Adenoviral RNA in Isolated Nuclei

Contact Info

Product

Resources

About