Transcription profiles of boron-deficiency-responsive genes in citrus rootstock root by suppression subtractive hybridization and cDNA microarray

Abstract

Boron (B) deficiency has seriously negative effect on citrus production. Carrizo citrange (CC) has been reported as a B-deficiency tolerant rootstock. However, the molecular mechanism of its B-deficiency tolerance remained not well-explored. To understand the molecular basis of citrus rootstock to B-deficiency, suppression subtractive hybridization (SSH) and microarray approaches were combined to identify the potential important or novel genes responsive to B-deficiency. Firstly four SSH libraries were constructed for the root tissue of two citrus rootstocks CC and Trifoliate orange (TO) to compare B-deficiency treated and non-treated plants. Then 7680 clones from these SSH libraries were used to construct a cDNA array and microarray analysis was carried out to verify the expression changes of these clones upon B-deficiency treatment at various time points compared to the corresponding controls. A total of 139 unigenes that were differentially expressed upon B-deficiency stress either in CC or TO were identified from microarray analysis, some of these genes have not previously been reported to be associated with B-deficiency stress. In this work, several genes involved in cell wall metabolism and transmembrane transport were identified to be highly regulated under B-deficiency stress, and a total of 23 metabolic pathways were affected by B-deficiency, especially the lignin biosynthesis pathway, nitrogen metabolism, and glycolytic pathway. All these results indicated that CC was more tolerant than TO to B-deficiency stress. The B-deficiency responsive genes identified in this study could provide further information for understanding the mechanisms of B-deficiency tolerance in citrus.

Physiological and Nutritional Responses of ‘HB’ Pummelo [Citrus grandis (L.) Osbeck ‘Hirado Buntan’] to the Combined Effects of Low pH Levels and Boron Deficiency

“…It has been proven that plant roots are affected by B deficiency as a result of the role of B in cell wall construction (Dell and Huang, 1997;Marschner, 1995). Similar results have also been reported for the roots of other citrus (Li et al, 2016;Mei et al, 2016;Zhou et al, 2014Zhou et al, , 2015Wu et al, 2018). Further plant anatomical investigations of the roots of citrus (Li et al, 2016;Mei et al, 2016;Zhou et al, 2015) suffering from B starvation stress have been performed, and these results indicated that the root cell and vessel walls were thickened under B deficiency conditions.…”

“…1). Corky split vein is a typical symptom of citrus that have been proved in various citrus while suffering from B starvation stress (Han et al, 2008;Liu et al, 2011b;Yang et al, 2013Yang et al, , 2015Zhou et al, 2014Zhou et al, , 2015. Other symptoms were also observed under B deficiency at different pH values, with more severe symptoms observed at pH 4.0 and pH 5.0 than at pH 6.0 (Fig.…”

With or Without You: Altered Plant Response to Boron-Deficiency in Hydroponically Grown Grapevines Infected by Grapevine Pinot Gris Virus Suggests a Relation Between Grapevine Leaf Mottling and Deformation Symptom Occurrence and Boron Plant Availability

“…In field, B availability can be affected by several factors, changing over time and the space. Given that gene transcriptional regulation occurs within few hours after the onset of stress (Zhou et al, 2015) and, as revealed by the hydroponic experiment, grapevine leaf tissues can be considered a good target for the study of plant response to B bioavailability, the transcript levels of the B transporters were measured in leaf collected immediately after GMLD symptom occurrence. While neither the GPGV variant nor the virus titer seemed to be linked to GLMD symptom expression, the differential regulation of VvBOR3, VvNIP5, and VvNIP6 genes between symptomatic and asymptomatic plants hints at the importance of changes in B homeostasis as a trigger for GLMD symptom expression.…”

Establishment and optimization of a hydroponic system for root morphological and nutritional analysis of citrus

“…Fluorescent dyelabeled cDNA and hybridization were prepared according to a previous published protocol (Guo et al, 2005). Microarray data and EST sequence analysis had been carried out during our previously published study (Zhou et al, 2015).…”