Transcription Profiling of Cultured<i>Acropora digitifera</i>Adult Cells Reveals the Existence of Ancestral Genome Regulatory Modules Underlying Pluripotency and Cell Differentiation in Cnidaria

Reyes-Bermudez, Alejandro; Hidaka, Michio; Mikheyev, andAlexander

doi:10.1093/gbe/evab008

Cited by 14 publications

(4 citation statements)

References 41 publications

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“…A recent study [81] suggested the presence of ASCs in the rapidly growing branch tips of Acropora digitfera based on the expression pattern of cells that were extracted and cultured from this region. These cultured cells exhibited overexpression of several stemness markers from the Nanos gene family previously known from Hydra's i-cells.…”

Section: Discussionmentioning

confidence: 99%

Coral Tissue Regeneration and Growth Is Associated with the Presence of Stem-like Cells

Levanoni,

Rosner,

Lapidot

et al. 2024

JMSE

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Members of the Cnidaria phylum were studied for centuries to depict the source of their unprecedented regeneration capacity. Although adult stem cells (ASCs) have been recognized in tissue growth/regeneration in many hydrozoans, there has not been any evidence of them in the ancestral Anthozoa class. This study sheds light on the development of epidermal epithelium expansion, akin to blastema, during tissue regeneration after small circular incisions (each 2.77 mm2) and during the natural expansion of tissue across a flat surface in the scleractinian coral Stylophora pistillata. Regeneration was completed within 9 days in 84.5% (n = 64) of the assays. About 35% of the samples regrew a single polyp, 60% showed no polyp regrowth, and approximately 6% exhibited multiple new polyps. We further used histological staining, pH3, Piwi immuno-histochemistry, and qPCR for eight stemness markers: Piwi-1, Nanos-1, Nanos-1-like, Tudor-5, Tudor-7, Boule, Sox-2, and Myc-1. The results revealed the formation of an “addendum”, an epidermal epithelium in the growing edges (in regenerating and normal-growing fronts) inhabited by a cluster of small cells featuring dense nuclei, resembling ASCs, many expressing pH3 as well as Piwi proteins. Most of the stemness genes tested were upregulated. These results indicate the participation of ASCs-like cells in tissue regeneration and growth in scleractinian corals.

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Section: Discussionmentioning

confidence: 99%

Coral Tissue Regeneration and Growth Is Associated with the Presence of Stem-like Cells

Levanoni,

Rosner,

Lapidot

et al. 2024

JMSE

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“…File 1 for recipe) (Gates and Muscatine 1992 , Frank et al 1994 , Domart-Coulon et al 2004 ) to remove loosely associated microbes and transferred to a 50 ml polypropylene tube with ∼10 ml of CafASW. Each sample was incubated for 7 days at 4°C on a rotary mixer in CafASW to induce detachment of cells from the skeleton (Gates and Muscatine 1992 , Domart-Coulon et al 2004 , Marshall and Clode 2004 , Helman et al 2008 , Reyes-Bermudez et al 2021 ). After the CafASW incubation, samples were moved to room temperature (RT) and supplemented with collagenase (Type IV, Sigma, Lecointe et al 2013 ) at a final concentration of 0.15% (w/v) and incubated for an additional 6 hrs at 27°C on an orbital incubator (Gates and Muscatine 1992 , Gates et al 1992 , Frank et al 1994 , Helman et al 2008 , Khalesi et al 2008 ).…”

Section: Methodsmentioning

confidence: 99%

A targeted approach to enrich host-associated bacteria for metagenomic sequencing

Dungan,

Tandon,

Jameson

et al. 2023

FEMS Microbes

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Multicellular eukaryotic organisms are hosts to communities of bacteria that reside on or inside their tissues. Often the eukaryotic members of the system contribute to high proportions of metagenomic sequencing reads, making it challenging to achieve sufficient sequencing depth to evaluate bacterial ecology. Stony corals are one such complex community; however, separation of bacterial from eukaryotic (primarily coral and algal symbiont) cells has so far not been successful. Using a combination of hybridization chain reaction fluorescence in situ hybridization and fluorescence activated cell sorting (HCR-FISH + FACS), we sorted two populations of bacteria from five genotypes of the coral Acropora loripes, targeting 1) Endozoicomonas spp, and 2) all other bacteria. NovaSeq sequencing resulted in 67-91 M reads per sample, 55–90% of which were identified as bacterial. Most reads were taxonomically assigned to the key coral-associated family, Endozoicomonadaceae, with Vibrionaceae also abundant. Endozoicomonadaceae were 5x more abundant in the ‘Endozoicomonas’ population, highlighting the success of the dual-labelling approach. This method effectively enriched coral samples for bacteria with <1% contamination from host and algal symbionts. The application of this method will allow researchers to decipher the functional potential of coral-associated bacteria. This method can also be adapted to accommodate other host-associated communities.

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“…However, the identification, isolation and characterization of aquatic invertebrate stem cells remains a major, typically species-specific, technical challenge. With few species having established protocols for the isolation of identified stem-like cells (Hayashi et al 2006;Sun et al 2007;Hemmrich et al 2012;Reyes-Bermudez et al 2021), their generalization and transfer by taking advantage of the vast diversity of specific approaches explored in other taxa (Table 1) appear particularly promising.…”

Section: Selecting Suitable Sources Of Cellsmentioning

confidence: 99%

<p>Current Knowledge on Stem Cells in Ascidians</p>

Vanni¹,

Anselmi²,

Ballarin³

et al. 2022

Advances in Aquatic Invertebrate Stem Cell Research

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He is a member of the editorial board of the Invertebrate Survival Journal and the European Journal of Zoology, and is a founding member of the Italian Association of Developmental and Comparative Immunobiology (IADCI). His main research interests are the evolution of innate immunity and the study of the cellular and molecular basis of immune responses in marine invertebrates, with particular reference to the role of hemocytes/coelomocytes in immune defense and, more generally, in the stress response. Most of his studies were carried out using the colonial ascidian Botryllus schlosseri as a model organism. His interest in stem cells is directly related to their role in hematopoiesis, as they assure the continuous renewal of the circulating immunocytes of marine invertebrates. He is the chair of the COST Action 16203 MARISTEM "Stem cells of marine/aquatic invertebrates: from basic research to innovative applications" that supported the publication of this book. He is the author or co-author of more than 130 peer-reviewed publications in scientific journals, co-editor of scientific books, including "Lessons in immunity: from singlecell organisms to mammals" (Elsevier, 2016), and guest editor of Special Issues, including: "Ancient immunity. phylogenetic emergence of recognition-defence mechanisms" (Biology (Basel), 2020-2021).Baruch (Buki) Rinkevich has been a professor and senior scientist at the

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Transcription Profiling of CulturedAcropora digitiferaAdult Cells Reveals the Existence of Ancestral Genome Regulatory Modules Underlying Pluripotency and Cell Differentiation in Cnidaria

Cited by 14 publications

References 41 publications

Coral Tissue Regeneration and Growth Is Associated with the Presence of Stem-like Cells

Coral Tissue Regeneration and Growth Is Associated with the Presence of Stem-like Cells

A targeted approach to enrich host-associated bacteria for metagenomic sequencing

<p>Current Knowledge on Stem Cells in Ascidians</p>

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