Transcriptional activation of EGFR by HOXB5 and its role in breast cancer cell invasion

Lee, Ji Yeon; Kim, Jie Min; Jeong, Da Som; Kim, Myoung Hee

doi:10.1016/j.bbrc.2018.08.071

Cited by 31 publications

(25 citation statements)

References 20 publications

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“…4A). HOXB5 is involved in the tumorigenesis and tumor progression of various human cancer types (41)(42)(43); therefore, this gene was selected for validation. The luciferase reporter assay was performed to corroborate the prediction.…”

Section: Mir-944 Directly Targets Hoxb5 Mrna In Tscc Cellsmentioning

confidence: 99%

Long non‑coding RNA PRNCR1 exerts oncogenic effects in�tongue squamous cell carcinoma in�vitro and in�vivo by sponging microRNA‑944 and thereby increasing HOXB5�expression

Zou

et al. 2020

Int J Mol Med

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A long non-coding RNA (lncRNA) called prostate cancer-associated non-coding RNA 1 (PRNCR1) serves crucial roles in the aggressive phenotypes of colorectal cancer and non-small cell lung cancer. However, there is little research on the expression profile, clinical value and detailed functions of PRNCR1 in tongue squamous cell carcinoma (TScc). The aim of the present study was to determine PRNCR1 expression in TScc and to examine the involvement of PRNCR1 in TScc progression. The molecular mechanisms behind the oncogenic effects of PRNCR1 in TScc cells were also investigated. PRNCR1 was revealed to be upregulated in TScc tumors and cell lines. The high PRNCR1 expression showed a significant correlation with tumor size, clinical stage, lymph node metastasis, and shorter overall survival times among patients with TScc. A PRNCR1-knockdown reduced TScc cell proliferation, migration and invasion, and increased apoptosis in vitro. Additionally, the PRNCR1-knockdown slowed down in vivo tumor growth of TScc cells. With regards to the mechanism, PRNCR1 acted as a competing endogenous RNA on microRNA-944 (miR-944) in TScc cells, and the effects of the PRNCR1-knockdown were reversed by an miR-944-knockdown. HOXB5 was validated as a direct target gene of miR-944 in TScc cells, and HOXB5 expression was found to be positively regulated by PRNCR1. Furthermore, resumption of HOXB5 expression reversed the tumor-suppressive actions of miR-944 in TScc cells. In conclusion, PRNCR1 acts as an oncogenic lncRNA in TScc through the upregulation of HOXB5 by sponging miR-944, thereby indicating a potential therapeutic target in TScc.

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Section: Mir-944 Directly Targets Hoxb5 Mrna In Tscc Cellsmentioning

confidence: 99%

Long non‑coding RNA PRNCR1 exerts oncogenic effects in�tongue squamous cell carcinoma in�vitro and in�vivo by sponging microRNA‑944 and thereby increasing HOXB5�expression

Zou

et al. 2020

Int J Mol Med

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“…Lee et al suggested that HOXB5 increased cell proliferation and invasiveness in estrogen receptor-(ER-) positive breast cancer [32]. More interestingly, this research group also found that HOXB5 could upregulate EGFR expression and thereby promote HOXB5driven invasion in ER-positive breast cancer [33]. In this work, we noted that EGFR was involved in cell cycle and proliferation and served as a pivotal hub gene in PPI analysis.…”

Section: Discussionmentioning

confidence: 50%

Identification of Potential Biomarkers Associated with Basal Cell Carcinoma

Liu

Bian

2020

BioMed Research International

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Purpose. This work is aimed at identifying several molecular markers correlated with the diagnosis and development of basal cell carcinoma (BCC). Methods. The available microarray datasets for BCC were obtained from the Gene Expression Omnibus (GEO) database, and differentially expressed genes (DEGs) were identified between BCC and healthy controls. Afterward, the functional enrichment analysis and protein-protein interaction (PPI) network analysis of these screened DEGs were performed. An external validation for the DEG expression level was also carried out, and receiver operating characteristic curve analysis was used to evaluate the diagnostic values of DEGs. Result. In total, five microarray datasets for BCC were downloaded and 804 DEGs (414 upregulated and 390 downregulated genes) were identified. Functional enrichment analysis showed that these genes including CYFIP2, HOXB5, EGFR, FOXN3, PTPN3, CDC20, MARCKSL1, FAS, and PTCH1 were closely correlated with the cell process and PTCH1 played central roles in the BCC signaling pathway. Moreover, EGFR was a hub gene in the PPI network. The expression changes of six genes (CYFIP2, HOXB5, FOXN3, PTPN3, MARCKSL1, and FAS) were validated by an external GSE74858 dataset analysis. Finally, ROC analysis revealed that CYFIP2, HOXB5, PTPN3, MARCKSL1, PTCH1, and CDC20 could distinguish BCC and healthy individuals. Conclusion. Nine gene signatures (CYFIP2, HOXB5, EGFR, FOXN3, PTPN3, CDC20, MARCKSL1, FAS, and PTCH1) may serve as promising targets for BCC detection and development.

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“…In contrast, we found ten genes to be over-expressed in old patients across at least three out of six tumor types. These include COQ3, EYA4, FER1L5, HOXB5, SYS1 and TSNAX and have been associated with a variety of cellular functions including mitochondrial function, tumorigenesis, memory formation and protein trafficking(Behnia et al, 2004; Cannon et al, 2005; Jonassen and Clarke, 2000; Lee et al, 2018). Altogether these findings indicate that primary tumors in younger patients have increased expression of tumor proliferation, progression, and metastasis genes.…”

Section: Resultsmentioning

confidence: 99%

Pan-cancer analysis reveals unique molecular patterns associated with age

Shah¹,

Verma²,

Marderstein³

et al. 2020

Preprint

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Older age is a strong risk factor for several diseases, including cancer. In cancer, older age is also frequently associated with a more aggressive, treatment-refractory tumor phenotype. The etiology and biology of age-associated differences among cancers are poorly understood. To address this knowledge gap, we sought to delineate the differences in tumor molecular characteristics between younger and older patients across a variety of tumor types. We found that tumors in younger and older patients exhibit widespread molecular differences. First, we observed that tumors in younger individuals, unlike those in older ones, exhibit an accelerated molecular aging phenotype associated with some hallmarks of premature senescence. Second, we found that tumors from younger individuals are enriched for driver gene mutations resulting in homologous recombination defects. Third, we observed a trend towards a decrease in immune infiltration and function in older patients and found that, immunologically, young tumor tissue resembles aged healthy tissue. Taken together, we find that tumors from young individuals possess unique characteristics compared to tumors in older individuals, which can potentially be leveraged for differential therapeutic strategies.

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Transcriptional activation of EGFR by HOXB5 and its role in breast cancer cell invasion

Cited by 31 publications

References 20 publications

Long non‑coding RNA PRNCR1 exerts oncogenic effects in�tongue squamous cell carcinoma in�vitro and in�vivo by sponging microRNA‑944 and thereby increasing HOXB5�expression

Long non‑coding RNA PRNCR1 exerts oncogenic effects in�tongue squamous cell carcinoma in�vitro and in�vivo by sponging microRNA‑944 and thereby increasing HOXB5�expression

Identification of Potential Biomarkers Associated with Basal Cell Carcinoma

Pan-cancer analysis reveals unique molecular patterns associated with age

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