Transcriptional activity and biological effects of mammalian estrogen receptor ligands on three hepatic estrogen receptors in Mozambique tilapia

Davis, Lori K.; Katsu, Yoshinao; Iguchi, Taisen; Lerner, Darren T.; Hirano, Tetsuya; Grau, E. Gordon

doi:10.1016/j.jsbmb.2010.05.009

Cited by 31 publications

(20 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, a recent study demonstrated that several subtype-specific ligands of mammalian ERs did not maintain the same selectivity in Mozambique tilapia ( Oreochromis mossambicus ), indicating that agonistic characteristics cannot always be extrapolated between species. 37 The YES assay is also notably unable to detect the estrogenic activity of chlorinated chemicals, which has led to its exclusion from the U.S. EPA EDSP. 38 However, this limitation is not applicable to the suite of estrogens used in this study.…”

Section: Discussionmentioning

confidence: 99%

Bridging the Gap From Screening Assays to Estrogenic Effects in Fish: Potential Roles of Multiple Estrogen Receptor Subtypes

Yost

Pow

Hawkins

et al. 2014

Environ. Sci. Technol.

View full text Add to dashboard Cite

This study seeks to delineate the ligand interactions that drive biomarker induction in fish exposed to estrogenic pollutants and provide a case study on the capacity of human (h) estrogen receptor (ER)-based in vitro screening assays to predict estrogenic effects in aquatic species. Adult male Japanese medaka (Oryzias latipes) were exposed to solutions of singular steroidal estrogens or to the estrogenic extract of an anaerobic swine waste lagoon. All exposure concentrations were calibrated to be equipotent based on the yeast estrogen screen (YES), which reports activation of hERα. These exposures elicited significantly different magnitudes of hepatic vitellogenin and choriogenin gene induction in the male medaka. Effects of the same YES-calibrated solutions in the T47D-KBluc assay, which reports activation of hERα and hERβ, generally recapitulated observations in medaka. Using competitive ligand binding assays, it was found that the magnitude of vitellogenin/choriogenin induction by different estrogenic ligands correlated positively with preferential binding affinity for medaka ERβ subtypes, which are highly expressed in male medaka liver prior to estrogen exposure. Results support emerging evidence that ERβ subtypes are critically involved in the teleost estrogenic response, with the ERα:ERβ ratio being of particular importance. Accordingly, incorporation of multiple ER subtypes into estrogen screening protocols may increase predictive value for the risk assessment of aquatic systems, including complex estrogenic mixtures.

show abstract

Section: Discussionmentioning

confidence: 99%

Bridging the Gap From Screening Assays to Estrogenic Effects in Fish: Potential Roles of Multiple Estrogen Receptor Subtypes

Yost

Pow

Hawkins

et al. 2014

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…We observed that the efficacy of steroidal estrogens in our assays was considerably greater than that observed in other recent studies examining ER transactivation. 30,38,42,61,65 To further assess these differences, we performed a follow-up experiment in which we evaluated the transactivation of all three mERs using a synthetic ERE reporters in the presence of E2β (data in Supporting Information). Analysis revealed that for all three mER subtypes, efficacy was larger with both pVTG reporters relative to synthetic ERE (Figure SI-4).…”

Section: Discussionmentioning

confidence: 99%

“…liver, gonads, brain, muscles, kidney) distribution patterns 28,30,37–41 and dissimilar tissue specific induction/repression patterns following estrogen exposure. 37,38,42–44 Although a breadth of knowledge has accumulated since the discovery of a third ER in teleost fish, the respective function of all ER subtypes in transactivation of estrogen responsive genes remains equivocal.…”

Section: Introductionmentioning

confidence: 99%

Sharing the Roles: An Assessment of Japanese Medaka Estrogen Receptors in Vitellogenin Induction

Pow

Yost

Aday

et al. 2016

Environ. Sci. Technol.

View full text Add to dashboard Cite

Teleost fish express at least three estrogen receptor (ER) subtypes. To date, however, the individual role of these ER subtypes in regulating expression of estrogen responsive genes remains ambiguous. Here, we investigate putative roles of three ER subtypes in Japanese medaka (Oryzias latipes), using vitellogenin (VTG) I and II as model genes. We identify specific ligand/receptor/promoter dynamics, using transient transactivation assays that incorporate luciferase reporters comprising 3kb promoter/enhancer regions of medaka VTGI and VTGII genes. Four steroidal estrogens (17β-estradiol, estrone, estriol, and 17α-estradiol) were tested in these assays. Results indicate that all three medaka ERs (mERs) are capable of initiating transactivation of both VTG I and II, with mERβ2 exhibiting the greatest efficacy. Promoter deletion analysis suggests that ligand-specific receptor transactivation and utilization of regional-specific estrogen response elements may be associated with differential activities of each medaka ER. Further, cluster analysis of in vivo gene expression and transactivation suggests that all three ER subtypes putatively play a role in up-regulation of VTG. Results illustrate that preferential ligand/receptor/promoter interactions may have direct implications for VTG gene expression and other ER-mediated regulatory functions that are relevant to the risk assessment of estrogenic compounds.

show abstract

“…Review of mammalian and teleost ER forms demonstrate that teleost ER-beta has a higher ligand affinity for estradiol than ER-alpha, which is opposite to that observed with mammalian ERs [28]. Additionally, Davis et al [29] demonstrate that functional characteristics of select ER-alpha and ER-beta agonistic and antagonistic (propyl-pyrazole-triol [PPT], diarylpropionitrile [DPN], and methyl-piperidino-pyrazole [MPP]) cannot be extrapolated between mammalian and teleost ERs.…”

Section: The Perspectives Column Is a Regular Series Designed To Discmentioning

confidence: 96%

In Summary

Brander

Hecht

Kuivila

2015

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Transcriptional activity and biological effects of mammalian estrogen receptor ligands on three hepatic estrogen receptors in Mozambique tilapia

Cited by 31 publications

References 42 publications

Bridging the Gap From Screening Assays to Estrogenic Effects in Fish: Potential Roles of Multiple Estrogen Receptor Subtypes

Bridging the Gap From Screening Assays to Estrogenic Effects in Fish: Potential Roles of Multiple Estrogen Receptor Subtypes

Sharing the Roles: An Assessment of Japanese Medaka Estrogen Receptors in Vitellogenin Induction

In Summary

Contact Info

Product

Resources

About