Transcriptional analysis of genes involved in competitive nodulation in Bradyrhizobium diazoefficiens at the presence of soybean root exudates

Liu, Yao; Jiang, Xin; Guan, Dawei; Zhou, Wei; Ma, Mingchao; Zhao, Baisuo; Cao, Fengming; Li, Li; Li, Jun

doi:10.1038/s41598-017-11372-0

Cited by 25 publications

(24 citation statements)

References 86 publications

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“…This different response is due to the different genes exist in a genotype. Some genes controlling soybean nodulation have been discovered, involving genes in soybean and rhizobia (Liu et al 2017;Tang et al 2016;Yan et al 2015). The inoculated plants produce higher nodule numbers, nodule weight, shoot and root biomass, eventually producing higher pod, stover, and seed yield (Alam et al 2015).…”

Section: Resultsmentioning

confidence: 99%

Agronomical performance of soybean genotypes infected by Cowpea Mild Mottle Virus in various level of nitrogen

2019

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Abstract. Nurrohman E, Zubaidah S, Kuswantoro H. 2019. Agronomical performance of soybean genotypes infected by Cowpea Mild Mottle Virus in various level of nitrogen. Biodiversitas 20: 1255-1263. Nitrogen is one of the essential nutrients during the process of vegetative and generative growth of soybean. Nitrogen deficiency during the growth can adverse to agronomical plant traits. This study was aimed to discover the influence of nitrogen on the agronomical traits of the soybean lines and varieties infected by cowpea mild mottle virus (CpMMV). The trial was arranged in factorial randomized complete block design with three replications. The first factor was the nitrogen dose consisting of four nitrogen doses while the soybean genotypes as the second factor consisted of seven genotypes. The results showed that nitrogen did not influence the plant resistance to CpMMV. The plant resistance was more affected by the genotype. The soybean genotypes were significant differences in the pod length, the pod width, and the flowering date. The interaction between genotype and nitrogen was found in the number of filled pods, the number of unfilled pods plant-1, the number of reproductive nodes plant-1, the number of total pods and the seed weight plant-1. The relationship in agronomic traits showed that significant positive correlations were found between number of filled pods with number of total pods, seed length with seed width and seed thickness, while the significant negative correlations were found between pod thickness with number of unfilled pods and maturity date with weight of 50 seeds.

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Section: Resultsmentioning

confidence: 99%

Agronomical performance of soybean genotypes infected by Cowpea Mild Mottle Virus in various level of nitrogen

2019

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“…In both seasons, rice farming is always done in waterlogged status. This provided an interesting insight particularly for the Philippines' agriculture since the strain B. diazoefficiens USDA110 T was proven to be a highly effective and efficient inoculant for soybean ( Siqueira et al., 2014 ; Liu et al., 2017 ) including its complete denitrification ability ( Itakura et al., 2013 ; Shiina et al., 2014 ; Akiyama et al., 2016 ) that is beneficial for climate change mitigation.…”

Section: Discussionmentioning

confidence: 99%

Influence of flooding and soil properties on the genetic diversity and distribution of indigenous soybean-nodulating bradyrhizobia in the Philippines

Mason

Tabing

Yamamoto

et al. 2018

Heliyon

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One of the strategies that is commonly used in the Philippines to improve the production of soybean is by inoculation. However, this technique often fails mainly due to the lack of information about the indigenous soybean rhizobia in the Philippines soil. In this study, the diversity of indigenous bradyrhizobia collected from the non-flooded and flooded soil conditions at 11 locations in the country was investigated using a local soybean cultivar as the host plant. The genetic variation among the 424 isolates was detected through Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) treatment and sequence analysis for 16S rRNA gene, 16S-23S rRNA internal transcribed spacer (ITS) region and rpoB housekeeping gene. All the isolates were classified under the Bradyrhizobium species namely B. elkanii, B. diazoefficiens, B. japonicum, B. yuanmingense and a considerable proportion of the isolates were clustered under Bradyrhizobium sp. The isolates which were classified under Bradyrhizobium sp. were thought to be endemic to Philippines soil as evidenced by their nucleotide divergence against the known rhizobia and the historical absence of rhizobia inoculation in the collection sites. The major influence on the distribution and diversity of soybean bradyrhizobia is attributed to the difference in the flooding period, followed by soil properties such as pH, soil type, and nutrient content. As determined, it is proposed that the major micro-symbiont of soybean in the Philippines are B. elkanii for non-flooded soils, then B. diazoefficiens and B. japonicum for flooded soils.

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“…In both season, rice farming is always done in waterlogged status. This provided an interesting insight particularly for Philippines agriculture since USDA110 T was proven to be a highly effective and efficient inoculant for soybean (21, 46) including its complete denitrification ability (2, 14, 41, 43) that is beneficial for climate change mitigation. The locations where these strains were isolated had no history of USDA110 inoculation so these strains are considered indigenous.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and distribution of indigenous soybean-nodulating bradyrhizobia in the Philippines

Mason

Tabing

Yamamoto

et al. 2018

Preprint

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18The diversity of indigenous bradyrhizobia from soils collected at 11 locations in the 19 Philippines was investigated using PSB-SY2 local soybean cultivar as the host plant. 20 Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) 21 treatment for 16S rRNA, 16S-23S rRNA internal transcribed spacer (ITS) region and rpoB 22 housekeeping gene was performed primarily to detect the genetic variation among the 424 23 isolates collected. Then, sequence analysis of 16S rRNA, ITS region and rpoB gene was 24 performed for the representative isolates. Majority of the isolates were classified under 25 Bradyrhizobium elkanii, B. diazoefficiens, B. japonicum, Bradyrhizobium sp., and few 26 isolates were related to B. yuanmingense. Genetic variations observed through PCR-RFLP 27 and sequence analyses of the ITS region and rpoB gene generally occurred in B. elkanii, 28 suggesting an occurrence of gene transfer. Shannon's diversity index showed varied results 29 with a lowest score of 0.00 and highest at 0.98 indicating a very diverse population of 30 bradyrhizobia across the country. Among all the factors considered in this work, soil 31 management such as period of flooding and some soil properties provided major influence 32 on the distribution and diversity of soybean bradyrhizobia in the country. Thus, it is 33 proposed that the major micro-symbiont of soybean in the Philippines are B. elkanii for 34 3 non-flooded soils, then B. diazoefficiens and B. japonicum for flooded soils. 35 Importance 36Agriculture production in the Philippines has been and is currently heavily dependent on 37 chemical inputs with mainly rice or corn mono-cropping that it rendered the soil acidic and 38 unproductive. Legume research in the country are mainly focused on plant varietal 39 improvements and very few are aimed at understanding the ecological niche of rhizobia 40 present in the soil. Since soybean has mutual relationship with rhizobia, this legume is a 41 good fallow crop or a rotation crop after rice and corn to help build up the nitrogen stock in 42 the soil. The significance of this research is the better understanding of the ecological niche 43 of indigenous soybean bradyrhizobia, particularly in a tropical archipelago like the 44 Philippines. This work was conceptualized with the utmost goal to increase soybean yield 45 by harnessing and evaluating the indigenous rhizobia in the soil to make production more 46 sustainable and human-friendly. 47 48 KEYWORDS 49 B. elkanii, B. diazoefficiens, B. japonicum, flooded soil, non-flooded, soil management 50 51 52 The genus Bradyrhizobium is a major micro-symbiont of soybean (Glycine max [L.] 53 Merrill) that could form nodule with legumes through symbiosis. The symbiotic 54 relationship between rhizobia and legume is a highly specific, complicated and 55 energy-exhaustive process (50) which may lead to improved crop yield if efficient and 56 sustained. It is considered that Bradyrhizobium is the predominant genus of rhizobia in the 57 tropics (9). Tropical regions ...

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Transcriptional analysis of genes involved in competitive nodulation in Bradyrhizobium diazoefficiens at the presence of soybean root exudates

Cited by 25 publications

References 86 publications

Agronomical performance of soybean genotypes infected by Cowpea Mild Mottle Virus in various level of nitrogen

Agronomical performance of soybean genotypes infected by Cowpea Mild Mottle Virus in various level of nitrogen

Influence of flooding and soil properties on the genetic diversity and distribution of indigenous soybean-nodulating bradyrhizobia in the Philippines

Genetic diversity and distribution of indigenous soybean-nodulating bradyrhizobia in the Philippines

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