Transcriptional analysis of selected cellulose-acting enzymes encoding genes of the white-rot fungus Dichomitus squalens on spruce wood and microcrystalline cellulose

Rytioja, Johanna; Hildén, Kristiina; Hatakka, Annele; Mäkelä, Miia

doi:10.1016/j.fgb.2013.12.008

Cited by 36 publications

(36 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…the cellobiohydrolase-encoding genes cel7C, cel7D, cel7F, celG, and cel6A is observed on cellotriose and cellotetraose cultures (23), suggesting that, in fact, the accumulated cellobiose may have caused induction of these genes. Microcrystalline cellulose (Avicel) has been found to upregulate the expression of cellobiohydrolase-encoding genes in D. squalens FBCC312, with the strongest effect on the gene cel7c (JGI MycoCosm protein ID 944872) (24,25). This observation is in agreement with our results determined for this particular gene in the cellobiose cultures of D. squalens FBCC312, indicating that the results from the Avicel cultures may in fact also reflect the induction by cellobiose released from this substrate.…”

Section: Discussionsupporting

confidence: 89%

Induction of Genes Encoding Plant Cell Wall-Degrading Carbohydrate-Active Enzymes by Lignocellulose-Derived Monosaccharides and Cellobiose in the White-Rot Fungus Dichomitus squalens

López

Peng

Issak

et al. 2018

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Fungi can decompose plant biomass into small oligo- and monosaccharides to be used as carbon sources. Some of these small molecules may induce metabolic pathways and the production of extracellular enzymes targeted for degradation of plant cell wall polymers. Despite extensive studies in ascomycete fungi, little is known about the nature of inducers for the lignocellulolytic systems of basidiomycetes. In this study, we analyzed six sugars known to induce the expression of lignocellulolytic genes in ascomycetes for their role as inducers in the basidiomycete white-rot fungus using a transcriptomic approach. This identified cellobiose and l-rhamnose as the main inducers of cellulolytic and pectinolytic genes, respectively, of Our results also identified differences in gene expression patterns between dikaryotic and monokaryotic strains of cultivated on plant biomass-derived monosaccharides and the disaccharide cellobiose. This suggests that despite conservation of the induction between these two genetic forms of, the fine-tuning in the gene regulation of lignocellulose conversion is differently organized in these strains. Wood-decomposing basidiomycete fungi have a major role in the global carbon cycle and are promising candidates for lignocellulosic biorefinery applications. However, information on which components trigger enzyme production is currently lacking, which is crucial for the efficient use of these fungi in biotechnology. In this study, transcriptomes of the white-rot fungus from plant biomass-derived monosaccharide and cellobiose cultures were studied to identify compounds that induce the expression of genes involved in plant biomass degradation.

show abstract

Section: Discussionsupporting

confidence: 89%

Induction of Genes Encoding Plant Cell Wall-Degrading Carbohydrate-Active Enzymes by Lignocellulose-Derived Monosaccharides and Cellobiose in the White-Rot Fungus Dichomitus squalens

López

Peng

Issak

et al. 2018

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…The barrier that lignin forms to impede the utilization of cellulose at the onset of wood degradation was also evident from the results of our study as expression of polysaccharide‐hydrolysing CAZyme‐ and LPMO‐encoding genes increased in time in wood. As reported previously (Rytioja et al ., ), D. squalens expressed all three genes encoding CBHI isoenzymes and the gene encoding CBHII in all the studied conditions. LPMO expression clustered with different sets of CAZymes and expansin‐like proteins, which is in line with their multifunctional nature in the degradation of various lignocellulose polymers (Westereng et al ., ).…”

Section: Discussionmentioning

confidence: 77%

“…The white‐rot fungus Dichomitus squalens is commonly found in the northern regions of Europe, Asia and North America (Andrews and Gill, ). Its genome possesses genes predicted to encode diverse plant cell wall‐modifying enzymes, and it has been shown to produce an extensive set of lignocellulose‐degrading enzymes (Floudas et al ., ; Rytioja et al ., ; Table ). In this study, we aimed to evaluate whether specialization of D. squalens to wood also resulted in a better molecular response in terms of a set of genes that fits better to the composition of wood substrates than to other plant biomass.…”

Section: Introductionmentioning

confidence: 95%

The molecular response of the white‐rot fungus Dichomitus squalens to wood and non‐woody biomass as examined by transcriptome and exoproteome analyses

Rytioja

Hildén

Falco

et al. 2017

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

The ability to obtain carbon and energy is a major requirement to exist in any environment. For several ascomycete fungi, (post-)genomic analyses have shown that species that occupy a large variety of habitats possess a diverse enzymatic machinery, while species with a specific habitat have a more focused enzyme repertoire that is well-adapted to the prevailing substrate. White-rot basidiomycete fungi also live in a specific habitat, as they are found exclusively in wood. In this study, we evaluated how well the enzymatic machinery of the white-rot fungus Dichomitus squalens is tailored to degrade its natural wood substrate. The transcriptome and exoproteome of D. squalens were analyzed after cultivation on two natural substrates, aspen and spruce wood, and two non-woody substrates, wheat bran and cotton seed hulls. D. squalens produced ligninolytic enzymes mainly at the early time point of the wood cultures, indicating the need to degrade lignin to get access to wood polysaccharides. Surprisingly, the response of the fungus to the non-woody polysaccharides was nearly as good a match to the substrate composition as observed for the wood polysaccharides. This indicates that D. squalens has preserved its ability to efficiently degrade plant biomass types not present in its natural habitat.

show abstract

“…A recent study shows that the genes encoding CBHs, LPMOs, and CDH are coexpressed when D. squalens grows on spruce wood and in microcrystalline cellulose (Avicel)-containing cultures. Moreover, the simultaneous expression of the cdh and lpmo genes emphasizes the role of oxidative degradation of cellulose together with hydrolytic cellulases in white rot fungi (61).…”

Section: Wood-rotting Fungimentioning

confidence: 99%

Plant-Polysaccharide-Degrading Enzymes from Basidiomycetes

Rytioja

Hildén

Yuzon

et al. 2014

Microbiol Mol Biol Rev

Self Cite

355

287

View full text Add to dashboard Cite

SUMMARY Basidiomycete fungi subsist on various types of plant material in diverse environments, from living and dead trees and forest litter to crops and grasses and to decaying plant matter in soils. Due to the variation in their natural carbon sources, basidiomycetes have highly varied plant-polysaccharide-degrading capabilities. This topic is not as well studied for basidiomycetes as for ascomycete fungi, which are the main sources of knowledge on fungal plant polysaccharide degradation. Research on plant-biomass-decaying fungi has focused on isolating enzymes for current and future applications, such as for the production of fuels, the food industry, and waste treatment. More recently, genomic studies of basidiomycete fungi have provided a profound view of the plant-biomass-degrading potential of wood-rotting, litter-decomposing, plant-pathogenic, and ectomycorrhizal (ECM) basidiomycetes. This review summarizes the current knowledge on plant polysaccharide depolymerization by basidiomycete species from diverse habitats. In addition, these data are compared to those for the most broadly studied ascomycete genus, Aspergillus , to provide insight into specific features of basidiomycetes with respect to plant polysaccharide degradation.

show abstract

Transcriptional analysis of selected cellulose-acting enzymes encoding genes of the white-rot fungus Dichomitus squalens on spruce wood and microcrystalline cellulose

Cited by 36 publications

References 55 publications

Induction of Genes Encoding Plant Cell Wall-Degrading Carbohydrate-Active Enzymes by Lignocellulose-Derived Monosaccharides and Cellobiose in the White-Rot Fungus Dichomitus squalens

Induction of Genes Encoding Plant Cell Wall-Degrading Carbohydrate-Active Enzymes by Lignocellulose-Derived Monosaccharides and Cellobiose in the White-Rot Fungus Dichomitus squalens

The molecular response of the white‐rot fungus Dichomitus squalens to wood and non‐woody biomass as examined by transcriptome and exoproteome analyses

Plant-Polysaccharide-Degrading Enzymes from Basidiomycetes

Contact Info

Product

Resources

About