Transcriptional Analysis of the
            <i>tutE tutFDGH</i>
            Gene Cluster from
            <i>Thauera aromatica</i>
            Strain T1

Coschigano, Peter W.

doi:10.1128/aem.66.3.1147-1151.2000

Cited by 32 publications

(34 citation statements)

References 27 publications

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“…The benzylsuccinate synthase is irreversibly inactivated in the presence of molecular oxygen by the oxygenolytic cleavage of the ␣-subunit (200,217). Whereas the bssD and bssCAB genes are cotranscribed in most bacteria, in T. aromatica strain T1, these genes were reported to be organized as two different operons, tutE and tutFDG (68). Two additional genes, bssE (tutH) and bssF, are also conserved in most bss clusters (Fig.…”

Section: Gene Clusters For Degradation Of Aromatic Hydrocarbonsmentioning

confidence: 99%

“…In the bss cluster of G. (203), Azoarcus sp. strain T (accession number AY032676) (1), T. aromatica K172 (accession numbers AJ001848 and AF173961) (155,216), T. aromatica T1 (accession numbers U57900 and AF113168) (68,72), Thauera sp. strain DNT-1 (accession number AB066263) (336), and G. metallireducens (accession number NC_007517) (48,202).…”

Section: Gene Clusters For Degradation Of Aromatic Hydrocarbonsmentioning

confidence: 99%

“…Consistent with this, it might be expected that the activator proteins are needed in smaller amounts than the activated proteins. Interestingly, in T. aromatica strain T1, the different amounts of the activator and benzylsuccinate synthase appear to be controlled transcriptionally by the existence of two different operons, tutE (bssD) and tutFDGE (bssCABE) (68).…”

Section: Effector-specific Regulationmentioning

confidence: 99%

See 2 more Smart Citations

Anaerobic Catabolism of Aromatic Compounds: a Genetic and Genomic View

Carmona

Zamarro

Blázquez

et al. 2009

Microbiol Mol Biol Rev

392

381

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SUMMARY Aromatic compounds belong to one of the most widely distributed classes of organic compounds in nature, and a significant number of xenobiotics belong to this family of compounds. Since many habitats containing large amounts of aromatic compounds are often anoxic, the anaerobic catabolism of aromatic compounds by microorganisms becomes crucial in biogeochemical cycles and in the sustainable development of the biosphere. The mineralization of aromatic compounds by facultative or obligate anaerobic bacteria can be coupled to anaerobic respiration with a variety of electron acceptors as well as to fermentation and anoxygenic photosynthesis. Since the redox potential of the electron-accepting system dictates the degradative strategy, there is wide biochemical diversity among anaerobic aromatic degraders. However, the genetic determinants of all these processes and the mechanisms involved in their regulation are much less studied. This review focuses on the recent findings that standard molecular biology approaches together with new high-throughput technologies (e.g., genome sequencing, transcriptomics, proteomics, and metagenomics) have provided regarding the genetics, regulation, ecophysiology, and evolution of anaerobic aromatic degradation pathways. These studies revealed that the anaerobic catabolism of aromatic compounds is more diverse and widespread than previously thought, and the complex metabolic and stress programs associated with the use of aromatic compounds under anaerobic conditions are starting to be unraveled. Anaerobic biotransformation processes based on unprecedented enzymes and pathways with novel metabolic capabilities, as well as the design of novel regulatory circuits and catabolic networks of great biotechnological potential in synthetic biology, are now feasible to approach.

show abstract

Section: Gene Clusters For Degradation Of Aromatic Hydrocarbonsmentioning

confidence: 99%

Section: Gene Clusters For Degradation Of Aromatic Hydrocarbonsmentioning

confidence: 99%

Section: Effector-specific Regulationmentioning

confidence: 99%

See 1 more Smart Citation

Anaerobic Catabolism of Aromatic Compounds: a Genetic and Genomic View

Carmona

Zamarro

Blázquez

et al. 2009

Microbiol Mol Biol Rev

392

381

View full text Add to dashboard Cite

show abstract

“…strain T (2), T. aromatica strain K172 (378), and T. aromatica strain T1 (135,136,137,378). In strain T. aromatica K172, the bbs (beta-oxidation of benzylsuccinate) operon contains bbsDCABE, with bbsCAB encoding the ␥, ␣, and ␤ subunits of benzylsuccinate synthase, a region with significant homology to the tutFDG genes in strain T1 (136,255,378).…”

Section: Anaerobic Hydrocarbon Metabolismmentioning

confidence: 99%

Recent Advances in Petroleum Microbiology

Hamme¹,

Singh

Ward

2003

Microbiol Mol Biol Rev

1,201

640

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Recent advances in molecular biology have extended our understanding of the metabolic processes related to microbial transformation of petroleum hydrocarbons. The physiological responses of microorganisms to the presence of hydrocarbons, including cell surface alterations and adaptive mechanisms for uptake and efflux of these substrates, have been characterized. New molecular techniques have enhanced our ability to investigate the dynamics of microbial communities in petroleum-impacted ecosystems. By establishing conditions which maximize rates and extents of microbial growth, hydrocarbon access, and transformation, highly accelerated and bioreactor-based petroleum waste degradation processes have been implemented. Biofilters capable of removing and biodegrading volatile petroleum contaminants in air streams with short substrate-microbe contact times (<60 s) are being used effectively. Microbes are being injected into partially spent petroleum reservoirs to enhance oil recovery. However, these microbial processes have not exhibited consistent and effective performance, primarily because of our inability to control conditions in the subsurface environment. Microbes may be exploited to break stable oilfield emulsions to produce pipeline quality oil. There is interest in replacing physical oil desulfurization processes with biodesulfurization methods through promotion of selective sulfur removal without degradation of associated carbon moieties. However, since microbes require an environment containing some water, a two-phase oil-water system must be established to optimize contact between the microbes and the hydrocarbon, and such an emulsion is not easily created with viscous crude oil. This challenge may be circumvented by application of the technology to more refined gasoline and diesel substrates, where aqueous-hydrocarbon emulsions are more easily generated. Molecular approaches are being used to broaden the substrate specificity and increase the rates and extents of desulfurization. Bacterial processes are being commercialized for removal of H2S and sulfoxides from petrochemical waste streams. Microbes also have potential for use in removal of nitrogen from crude oil leading to reduced nitric oxide emissions provided that technical problems similar to those experienced in biodesulfurization can be solved. Enzymes are being exploited to produce added-value products from petroleum substrates, and bacterial biosensors are being used to analyze petroleum-contaminated environments

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“…tutFDG are cotranscribed with tutH, a gene downstream of tutG whose predicted amino acid sequence is similar to that of the NorQ/NirQ family of proteins. No tutFDGH mRNA was observed when cells were grown on pyruvate (8). No sequence data downstream of bssDCAB have been reported for T. aromatica strain K172, and it is unknown if a tutH homolog exists in T. aromatica strain K172.…”

mentioning

confidence: 99%

Benzylsuccinate Synthase of Azoarcus sp. Strain T: Cloning, Sequencing, Transcriptional Organization, and Its Role in Anaerobic Toluene and m -Xylene Mineralization

Achong¹,

Rodrı́guez²,

Spormann

2001

J Bacteriol

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Biochemical studies in Azoarcus sp. strain T have demonstrated that anaerobic oxidation of both toluene and m-xylene is initiated by addition of the aromatic hydrocarbon to fumarate, forming benzylsuccinate and 3-methyl benzylsuccinate, respectively. Partially purified benzylsuccinate synthase was previously shown to catalyze both of these addition reactions. In this study, we identified and sequenced the genes encoding benzylsuccinate synthase from Azoarcus sp. strain T and examined the role of this enzyme in both anaerobic toluene and m-xylene mineralization. Based on reverse transcription-PCR experiments and transcriptional start site mapping, we found that the structural genes encoding benzylsuccinate synthase, bssCAB, together with two additional genes, bssD and bssE, were organized in an operon in the order bssDCABE. bssD is believed to encode an activating enzyme, similar in function to pyruvate formate-lyase activase. bssE shows homology to tutH from Thauera aromatica strain T1, whose function is currently unknown. A second operon that is upstream of bssDCABE and divergently transcribed contains two genes, tdiS and tdiR. The predicted amino acid sequences show similarity to sensor kinase and response regulator proteins of prokaryotic two-component regulatory systems. A chromosomal null bssA mutant was constructed (the bssA gene encodes the ␣-subunit of benzylsuccinate synthase). This bssA null mutant strain was unable to grow under denitrifying conditions on either toluene or m-xylene, while growth on benzoate was unaffected. The growth phenotype of the ⌬bssA mutant could be rescued by reintroducing bssA in trans. These results demonstrate that benzylsuccinate synthase catalyzes the first step in anaerobic mineralization of both toluene and m-xylene.

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Transcriptional Analysis of the tutE tutFDGH Gene Cluster from Thauera aromatica Strain T1

Cited by 32 publications

References 27 publications

Anaerobic Catabolism of Aromatic Compounds: a Genetic and Genomic View

Anaerobic Catabolism of Aromatic Compounds: a Genetic and Genomic View

Recent Advances in Petroleum Microbiology

Benzylsuccinate Synthase of Azoarcus sp. Strain T: Cloning, Sequencing, Transcriptional Organization, and Its Role in Anaerobic Toluene and m -Xylene Mineralization

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