Transcriptional and biochemical markers in transplanted Perca flavescens to characterize cadmium- and copper-induced oxidative stress in the field

Defo, Michel Amery; Bernatchez, Louis; Campbell, Peter G. C.; Couture, Patrice

doi:10.1016/j.aquatox.2015.02.014

Cited by 21 publications

(8 citation statements)

References 55 publications

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“…On the other hand, we found sharply increased mRNA levels were accompanied by unchanged activity and protein levels of CAT under Zn stress, suggesting that CAT gene may be regulated by Zn at a translational level. It is possible that regulation of CAT transcription is only sufficient to maintain its activity (Defo et al, 2015). It is not surprising that there are different responses at transcriptional and catalytic levels of some antioxidant genes (Craig et al, 2007;Giuliani et al, 2013;Hansen et al, 2007;Regoli and Giuliani, 2014;Zheng et al, 2016c).…”

Section: Discussionmentioning

confidence: 98%

Chronic waterborne zinc and cadmium exposures induced different responses towards oxidative stress in the liver of zebrafish

et al. 2016

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Section: Discussionmentioning

confidence: 98%

Chronic waterborne zinc and cadmium exposures induced different responses towards oxidative stress in the liver of zebrafish

et al. 2016

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“…Antioxidant response was measured at the gene transcription level and showed that genes coding for glutathione peroxidases were down-regulated in the muscle (gpx4b) and liver (gpx4) of yellow perch in LSP (Table 1). However, in a recent study, antioxidant enzyme activities of glutathione-Stransferase (GST) and glucose-6-phosphate dehydrogenase (G6PDH) were found to be significantly induced in yellow perch exposed in vivo to Cu and Cd (Defo et al 2015). Similar results were also observed in golden grey mullets (Liza aurata) exposed to Hg, with an increased activity of antioxidant enzymes, which resulted in the prevention of lipid peroxidation (Brandao et al 2015).…”

Section: Lipid Peroxidationmentioning

confidence: 61%

Integrated spatial health assessment of yellow perch (Perca flavescens) populations from the St. Lawrence River (QC, Canada), part B: cellular and transcriptomic effects

Bruneau

Landry

Giraudo

et al. 2016

Environ Sci Pollut Res

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Multi-biological level assessments have become great tools to evaluate the health of aquatic ecosystems. Using this approach, a complementary study was designed to evaluate the health of yellow perch (Perca flavescens) populations in the St. Lawrence River (Quebec, Canada). In the present study, stress responses were compared at the transcriptomic, cellular, and tissue levels in yellow perch collected at six sites along the river: Lake St. François, Lake St. Louis (north and south), Beauregard Island and Lake St. Pierre (north and south). These results complement the physiological and chemical parameters as well as pathogen infection investigated in a companion paper published in the present issue. Thiobarbituric acid reactive substance (TBARS) analyses indicated the presence of oxidative stress in fish collected in the southern part of Lake St. Louis and at the downstream sites of Lake St. Pierre. High lipid peroxidation levels were found in the muscle of yellow perch caught at Beauregard Island, located downstream of the Montreal's wastewater treatment plant, suggesting an impact of the municipal effluent on redox homeostasis. Transcriptomic results indicated the down-regulation of genes related to lipid, glucose, and retinoid in southern Lake St. Pierre as well as a decrease in retinoid storage. Overall, biochemical and molecular markers indicated that the health status of yellow perch followed a decreasing gradient from upstream to downstream of the St. Lawrence River. This gradient is representative of the cumulative negative impacts of human activities on water and habitat quality along the river.

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“…Thus, the synchronous responses obtained at the molecular and biochemical levels reflect a fight against metal-induced oxidative stress and suggest that in an environment contaminated by a cocktail of metals, the regulation of the G6PDH metabolic pathway occurs at both levels of biological organisation. In support of this hypothesis, liver transcription levels of g6pdh were increased in clean yellow perch transplanted into a metal-contaminated lake (high Cd concentration) and this up-regulation was accompanied by an increase in G6PDH activity (Defo et al, 2015). Furthermore, six weeks of exposure of yellow perch to Cd in the laboratory led to an increase of the liver g6pdh transcription level followed by a corresponding increase in enzyme activity (Defo et al, 2014a).…”

Section: Livermentioning

confidence: 74%

“…However, because of some limitations in their study design, such as a difference in sampling years, it is not possible to use their results to evaluate the possible seasonal variation in G6PDH activity in fish living in metal-impacted lakes. Note that in a later study, carried out in the spring of 2012, liver transcription levels of g6pdh were enhanced in wild yellow perch collected from a clean lake and then caged in a metal-contaminated lake, and this up-regulation was accompanied by an increase in the activity of the corresponding enzyme (Defo et al, 2015).…”

Section: Introductionmentioning

confidence: 79%

“…In a field study carried out in the spring, we observed that the liver transcription level of microsomal gst-3 decreased in metal-contaminated yellow perch compared to control fish, although no change in the activity of the corresponding enzyme was found. The activity of liver CAT increased in those fish, but this variation was not reflected by the cat gene transcription level (Defo et al, 2015). Bougas et al (2016) reported that genes encoding for antioxidantrelated proteins, namely Cu/Zn sod, gst-mu3 and mgst were overexpressed in liver of uncontaminated yellow perch chronically transplanted into a metal-contaminated lake for 4…”

Section: Introductionmentioning

confidence: 99%

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Temporal variations in kidney metal concentrations and their implications for retinoid metabolism and oxidative stress response in wild yellow perch (Perca flavescens)

et al. 2018

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The objective of this study was to determine if temporal variations in tissue metal concentrations are related to biomarkers of retinoid metabolism and oxidative stress responses in juvenile yellow perch (Perca flavescens). To this end, kidney metal (Cd, Cu and Zn) concentrations were measured in fish sampled in spring and fall 2012 in four lakes representing a wide range of water and sediment metal contamination in the Rouyn-Noranda (Quebec) region. Lakes Opasatica and Hélène were considered as reference lakes while lakes Dufault and Marlon were metal-contaminated. Kidney concentrations of Cd, Cu and Zn varied widely between spring and fall in fish from both clean and metal-contaminated lakes. An inter-lake difference in renal metal concentrations was only observed for Cd, with fish from Lake Marlon consistently displaying higher concentrations. In the spring, the concentrations of liver dehydroretinol, dehydroretinyl palmitate and total vitamin A esters were higher in fish sampled in the most contaminated lake. Strong temporal variations in the concentrations of these metabolites, as well as in the percentage of liver free dehydroretinol and the epidermal retinol dehydrogenase 2 transcription levels, were observed in fish living in the most metal-impacted lake, with generally higher values in the spring. In contrast to liver, in muscle, no clear seasonal variations in the concentrations of dehydroretinol, dehydroretinyl stearate or in the percentage of free dehydroretinol were observed in fish captured in the most contaminated lake. Temporal variations of traditional biomarkers of oxidative stress response were also observed in the most metal-impacted lake. For example, the transcription level of the gene encoding Cu/Zn superoxide dismutase-1 in liver and muscle catalase activity of perch sampled in the most contaminated lake were higher in spring than in fall. Positive relationships were found between kidney Cd concentrations and the transcription level of the gene encoding glucose 6-phosphate dehydrogenase, and all forms of retinoid concentrations in liver in spring, except with the percentage of free dehydroretinol where the correlation was negative. Our results translate to a state of stress caused by Cd and illustrate that temporal variations in tissue metal concentrations affect retinoid metabolism and antioxidant capacities in juvenile wild yellow perch. Overall this study contributes to highlight the importance of considering temporal variations when investigating the consequences of metal contamination on the physiology of wild fish.

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Transcriptional and biochemical markers in transplanted Perca flavescens to characterize cadmium- and copper-induced oxidative stress in the field

Cited by 21 publications

References 55 publications

Chronic waterborne zinc and cadmium exposures induced different responses towards oxidative stress in the liver of zebrafish

Chronic waterborne zinc and cadmium exposures induced different responses towards oxidative stress in the liver of zebrafish

Integrated spatial health assessment of yellow perch (Perca flavescens) populations from the St. Lawrence River (QC, Canada), part B: cellular and transcriptomic effects

Temporal variations in kidney metal concentrations and their implications for retinoid metabolism and oxidative stress response in wild yellow perch (Perca flavescens)

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