Transcriptional and translational study of the Drosophila subobscura hsp83 gene in normal and heat-shock conditions

Abstract

In this paper we report a transcriptional and translational study of the hsp83 gene of Drosophila subobscura. This gene is located at the 18C region of the J chromosome. A monoclonal antibody raised against hsp83 was used for the immunological detection of this protein by Western blotting throughout the development of D. subobscura in control and heat-shock conditions. Our results indicate that puff 18C is not only heat-shock inducible but is also expressed during normal development and its level of expression increases at the end of the prepupa period. We detected hsp83 at normal temperatures, in particular developmental stages with the exception of the larval and the beginning of prepupa formation. Hsp83 was induced by heat shock in all stages studied with the exception of 1st instar larvae. We found that temperatures in excess of 26 degrees C were sufficient to induce hsp83. In addition, at temperatures from 26 to 34 degrees C, the increase in hsp83 synthesis was accompanied by increased transcription of the hsp83 gene; this positive correlation was not observed at 37 degrees C.

Thermal evolution of gene expression profiles in Drosophila subobscura

“…It is also worth saying that the Hsp60 gene had a q -value threshold of 0.082 (P13 > P22) and might have also been associated with thermal adaptation. On the other hand, Hsp83 is known to be expressed during normal development in D. subobscura but increased transcription occurs when flies are reared at heat-shock temperatures from 26 to 34°C [58]. Consistent with this finding we did not observe a statistically significant differential gene expression between cold- and warm-adapted populations for Hsp83 ( q -value threshold 0.467).…”

“…The highest experimental temperature used in our stocks (22°C) was not expected to be stressful. This was apparently confirmed by the lack of induction in the P22 populations of a heat shock response known to occur in D. subobscura [58]. The data also suggest an association with polymorphic inversions as some clustering of genes within inverted chromosomal sections was detected.…”

Ultrastructure of regions containing homologous loci in polytene chromosomes of Drosophila melanogaster and Drosophila subobscura

“…Two hybridisation signals in the subsection 89A have been detected, while a single site was described by Moltó et al (1992). On the other hand we corroborate the location given for the Hsp82 gene by Arbona et al (1993), in the penultimate band of subdivision 18C. For the rest of the hsp genes we have given a more precise description of the site of hybridisation.…”

“…We can give its precise location: band 63B9. In situ hybridisation experiments in D. subobscura by Arbona et al (1993) also give the same location: a distal faint band in subsection 18C. In both species the probe hybridises to a faint band.…”

Heat‐shock protein 90 is down‐regulated during pupal diapause in the flesh fly, Sarcophaga crassipalpis, but remains responsive to thermal stress

“…, 1990). In Drosophila , it is constitutively expressed at low levels under normal conditions (Arbona et al. , 1993), with dramatic increases documented in response to thermal stress and other environmental insults (Yiangou et al.…”