Transcriptional changes of gibberellin oxidase genes in grapevines with or without gibberellin application during inflorescence development

Jung, Chan Jin; Hur, Youn Young; Jung, Sung-Min; Noh, Jung-Ho; Do, Gyung-Ran; Park, Seo-June; Nam, Jong-Chul; Park, Kyo-Sun; HaeSung, Hwang; Choi, Doil; Lee, Hee Jae

doi:10.1007/s10265-013-0623-x

Cited by 18 publications

(16 citation statements)

References 59 publications

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“…These results imply that VvIAA9 , VvARF7 , and VvARF8 function as negative regulators of fruit set initiation, whose down-regulation leads to fruit set initiation. With GA application, however, VvIAA9 and VvARF7 were down-regulated 2 days after the application, and their transcription levels remained low until full bloom or 2 DBF, respectively (Figure 2A–B), in which expression levels of GA biosynthesis genes were down-regulated by GA application at 14 DBF [37]. These results indicate that effects of GA application at pre-bloom were not limited on transcriptional changes of these genes at 1 or 2 days after the application.…”

Section: Discussionmentioning

confidence: 99%

“…However, although GA-mediated parthenocarpy is a highly desirable trait for table grapes, how GA induces parthenocarpic fruit development remains unclear. GA application at the pre-bloom stage in grapevines inhibited pollen tube growth and disturbed the balance of GA metabolism at near full bloom [37]. In the present study, changes in the expression levels of the fruit set related genes, VvIAA9 , VvARF7 , VvARF8 , and VvDELLA in grapevines were monitored to determine whether GA application coordinates auxin signaling during parthenocarpic fruit development.…”

Section: Introductionmentioning

confidence: 93%

“…The effects of GA application have been studied on early ripening and berry enlargement [32]–[34], and on the appropriate application timing at the pre-bloom stage for the induction of seedless grapes [31], [35]. On a molecular level, both GA and auxin biosynthesis genes are up-regulated after pollination in grapevines [3], [36], [37]. However, although GA-mediated parthenocarpy is a highly desirable trait for table grapes, how GA induces parthenocarpic fruit development remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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Gibberellin Application at Pre-Bloom in Grapevines Down-Regulates the Expressions of VvIAA9 and VvARF7, Negative Regulators of Fruit Set Initiation, during Parthenocarpic Fruit Development

Jung

Hur

et al. 2014

PLoS ONE

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Fruit set is initiated only after fertilization and is tightly regulated primarily by gibberellins (GAs) and auxins. The application of either of these hormones induces parthenocarpy, fruit set without fertilization, but the molecular mechanism underlying this induction is poorly understood. In the present study, we have shown that the parthenocarpic fruits induced by GA application at pre-bloom result from the interaction of GA with auxin signaling. The transcriptional levels of the putative negative regulators of fruit set initiation, including Vitis auxin/indole-3-acetic acid transcription factor 9 (VvIAA9), Vitis auxin response factor 7 (VvARF7), and VvARF8 were monitored during inflorescence development in seeded diploid ‘Tamnara’ grapevines with or without GA application. Without GA application, VvIAA9, VvARF7, and VvARF8 were expressed at a relatively high level before full bloom, but decreased thereafter following pollination. After GA application at 14 days before full bloom (DBF); however, the expression levels of VvIAA9 and VvARF7 declined at 5 DBF prior to pollination. The effects of GA application on auxin levels or auxin signaling were also analyzed by monitoring the expression patterns of auxin biosynthesis genes and auxin-responsive genes with or without GA application. Transcription levels of the auxin biosynthesis genes Vitis anthranilate synthase β subunit (VvASB1-like), Vitis YUCCA2 (VvYUC2), and VvYUC6 were not significantly changed by GA application. However, the expressions of Vitis Gretchen Hagen3.2 (VvGH3.2) and VvGH3.3, auxin-responsive genes, were up-regulated from 2 DBF to full bloom with GA application. Furthermore, the Vitis GA signaling gene, VvDELLA was up-regulated by GA application during 12 DBF to 7 DBF, prior to down-regulation of VvIAA9 and VvARF7. These results suggest that VvIAA9 and VvARF7 are negative regulators of fruit set initiation in grapevines, and GA signaling is integrated with auxin signaling via VvDELLA during parthenocarpic fruit development in grapevines.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Gibberellin Application at Pre-Bloom in Grapevines Down-Regulates the Expressions of VvIAA9 and VvARF7, Negative Regulators of Fruit Set Initiation, during Parthenocarpic Fruit Development

Jung

Hur

et al. 2014

PLoS ONE

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“…To elucidate the molecular sources of the varietal differences in bioactive GA quantities described above, the spatio-temporal transcript levels of the rate-limiting 2-oxoglutarate-dependent dioxygenases (2-ODDs) gene families of grapevine ( VvGA20ox, VvGA3ox, VvGA2ox ) (Giacomelli et al, 2013; Jung et al, 2014) were quantified by qRT-PCR ( Figures 8A–C and Supplementary Figure S5). For consistency, the nomenclature of genes used in this study is same as reported in our previous study (Giacomelli et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Abnormal Endogenous Repression of GA Signaling in a Seedless Table Grape Cultivar with High Berry Growth Response to GA Application

et al. 2017

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Gibberellin (GA) application is routinely used in the table grape industry to increase berry size and cluster length. Although grapevine cultivars show a wide range of growth responsiveness to GA3 application, the reasons for these differences is unclear. To shed light on this issue, two commercial grapevine cultivars with contrasting berry response to GA were selected for comparative analysis, in which we tested if the differences in response: (1) is organ-specific or cultivar-related; (2) will be reflected in qualitative/quantitative differences in transcripts/proteins of central components of GA metabolism and signaling and levels of GA metabolites. Our results showed that in addition to the high response of its berries to GA, internodes and rachis of cv. Black finger (BF) presented a greater growth response compared to that of cv. Spring blush (SB). In agreement, the results exposed significant quantitative differences in GA signaling components in several organs of both cultivars. Exceptionally higher level of all three functional VvDELLA proteins was recorded in young BF organs, accompanied by elevated VvGID1 expression and lower VvSLY1b transcripts. Absence of seed traces, low endogenous GA quantities and lower expression of VvGA20ox4 and VvGA3ox3 were also recorded in berries of BF. Our results raise the hypothesis that, in young organs of BF, low expression of VvSLY1b may be responsible for the massive accumulation of VvDELLA proteins, which then leads to elevated VvGID1 levels. This integrated analysis suggests causal relationship between endogenous mechanisms leading to anomalous GA signaling repression in BF, manifested by high quantities of VvDELLA proteins, and greater growth response to GA application.

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“…o efeito desses reguladores, em uvas apirenas, está vinculado, principalmente, ao aumento da divisão e da expansão celular (JunG et al, 2014). o efeito da giberelina é variável em função da cultivarcopa e do porta-enxerto, concentração, modo e época de aplicação e das condições ambientais (teCChio et al, 2009 ).…”

Section: Introductionunclassified

Influência Do Ácido Giberélico Na Fisiologia E Qualidade Da Videira Cv Sweet Celebration No Submédio São Francisco

et al. 2015

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RESUMO -o objetivo deste trabalho foi avaliar o efeito da aplicação de doses únicas e fracionadas de ácido giberélico nas trocas gasosas e fotossíntese e na qualidade de cachos da uva cv. Sweet Celebration, no Submédio São Francisco. o delineamento experimental foi o em blocos ao acaso, com 10 tratamentos (testemunha; uma, duas ou três aplicações de 10 mg. INFLUENCE OF GIBBERELLIC ACID ON PHYSIOLOGY AND QUALITY OF VINE CV SWEET CELEBRATION ® ON SUBMEDIUM SÃO FRANCISCOABSTRACT-the goal of this study was to show the effect of the application of single dose and fractionated dose of gibberellic acid at the gas exchange and photosynthesis and in the quality of bunch of the cv. Sweet Celebration grapes, in the submedium São Francisco. the experimental design was in randomized block, with 10 treatments (control; one, two or three applications of 10 mg.L -1 ; one, two or three applications of 15 mg.L -1 ; single application of 20 mg.L -1 , of 30 mg.L -1 or of 45 mg.L -1 ), with four replications, each composed of three plants. the gibberellic acid was applied in the phase of berries with 8 mm, increased the weight and the size of the grapes without influencing the soluble solids and titratable acidity. However, the larger mass (9,41 g), length (26,8 mm) and volume (8,12 cm 3 ) of berries were according to three applications of 10 mg.L -1 resulting in the increments, respectively, 59%, 23% and 42.5%, when compared to the control. the three applications of gibberellic acid in the different phases of development of bunches, favored the photosynthetic activity and the quality of bunches and berries.

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Transcriptional changes of gibberellin oxidase genes in grapevines with or without gibberellin application during inflorescence development

Cited by 18 publications

References 59 publications

Gibberellin Application at Pre-Bloom in Grapevines Down-Regulates the Expressions of VvIAA9 and VvARF7, Negative Regulators of Fruit Set Initiation, during Parthenocarpic Fruit Development

Gibberellin Application at Pre-Bloom in Grapevines Down-Regulates the Expressions of VvIAA9 and VvARF7, Negative Regulators of Fruit Set Initiation, during Parthenocarpic Fruit Development

Abnormal Endogenous Repression of GA Signaling in a Seedless Table Grape Cultivar with High Berry Growth Response to GA Application

Influência Do Ácido Giberélico Na Fisiologia E Qualidade Da Videira Cv Sweet Celebration No Submédio São Francisco

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