Transcriptional changes of mouse ovary during follicle initial or cyclic recruitment mediated by extra hormone treatment

Bian, Xiaolan; Xie, Qin; Zhou, Yuxiao; Wu, Haibo; Cui, Junqi; Jia, Liling; Suo, Lun

doi:10.1016/j.lfs.2020.118654

Cited by 9 publications

(5 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, double SOX3 deletion in female mice led to excess follicular atresia, ovulation of defective oocytes, and severely reduced fertility (Weiss et al., 2003 ). SOX‐3 was also identified as one of eight master transcription factors driving folliculogenesis in mice (Bian et al., 2021 ) and was found to inhibit apoptosis during follicle development in zebrafish leading to improved fecundity (Hong et al., 2019 ). In general, SOX3 constitutes a gene of high relevance for reproductive functioning, that is crucial for normal development and functioning of the hypothalamo‐pituitary axis.…”

Section: Discussionmentioning

confidence: 99%

Genetic and epigenetic differentiation in response to genomic selection for avian lay date

Lindner,

Verhagen,

Mateman

et al. 2024

Evolutionary Applications

View full text Add to dashboard Cite

Anthropogenic climate change has led to globally increasing temperatures at an unprecedented pace and, to persist, wild species have to adapt to their changing world. We, however, often fail to derive reliable predictions of species' adaptive potential. Genomic selection represents a powerful tool to investigate the adaptive potential of a species, but constitutes a ‘blind process’ with regard to the underlying genomic architecture of the relevant phenotypes. Here, we used great tit (Parus major) females from a genomic selection experiment for avian lay date to zoom into this blind process. We aimed to identify the genetic variants that responded to genomic selection and epigenetic variants that accompanied this response and, this way, might reflect heritable genetic variation at the epigenetic level. We applied whole genome bisulfite sequencing to blood samples of individual great tit females from the third generation of bidirectional genomic selection lines for early and late lay date. Genomic selection resulted in differences at both the genetic and epigenetic level. Genetic variants that showed signatures of selection were located within genes mostly linked to brain development and functioning, including LOC107203824 (SOX3‐like). SOX3 is a transcription factor that is required for normal hypothalamo‐pituitary axis development and functioning, an essential part of the reproductive axis. As for epigenetic differentiation, the early selection line showed hypomethylation relative to the late selection line. Sites with differential DNA methylation were located in genes important for various biological processes, including gonadal functioning (e.g., MSTN and PIK3CB). Overall, genomic selection for avian lay date provided insights into where within the genome the heritable genetic variation for lay date, on which selection can operate, resides and indicates that some of this variation might be reflected by epigenetic variants.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic and epigenetic differentiation in response to genomic selection for avian lay date

Lindner,

Verhagen,

Mateman

et al. 2024

Evolutionary Applications

View full text Add to dashboard Cite

show abstract

“…Furthermore, this study presents a granulosa cell-focused investigation of primordial follicle activation in mice. Previous mouse model studies using bulk RNA-seq to capture primordial follicle activation or early ovarian time points analyze a compendium of cell types and do not focus specifically on the granulosa cell to granulosa cell transition [ 48 , 49 ]. Single cell sequencing has, so far, been underutilized in the study of primordial follicle activation and lacking depth in the analysis of granulosa cells during this important physiological event [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic profiling of neonatal mouse granulosa cells reveals new insights into primordial follicle activation

Ford

Frost

Beckett

et al. 2021

Biology of Reproduction

View full text Add to dashboard Cite

The dormant population of ovarian primordial follicles is determined at birth and serves as the reservoir for future female fertility. Yet our understanding of the molecular, biochemical, and cellular processes underpinning primordial follicle activation remains limited. The survival of primordial follicles relies on the correct complement and morphology of granulosa cells, which provide signalling factors essential for oocyte and follicular survival. To investigate the contribution of granulosa cells in the primordial-to-primary follicle transition, gene expression profiles of granulosa cells undergoing early differentiation were assessed in a murine model. Ovaries from C57Bl/6 mice were enzymatically dissociated at time-points spanning the initial wave of primordial follicle activation. Post-natal day (PND) 1 ovaries yielded primordial granulosa cells, and PND4 ovaries yielded a mixed population of primordial and primary granulosa cells. The comparative transcriptome of granulosa cells at these time-points was generated via Illumina NextSeq 500 system which identified 131 significantly differentially expressed transcripts. The differential expression of eight of the transcripts was confirmed by RT-qPCR Following biological network mapping via Ingenuity Pathway Analysis, the functional expression of the protein products of three of the differentially expressed genes, namely FRZB, POD1 and ZFX, was investigated with in-situ immunolocalisation in PND4 mouse ovaries was investigated. Finally, evidence was provided that Wnt pathway antagonist, secreted frizzled-related protein 3 (FRZB), interacts with a suppressor of primordial follicle activation WNT3A and may be involved in promoting primordial follicle activation. This study highlights the dynamic changes in gene expression of granulosa cells during primordial follicle activation and provides evidence for a renewed focus into the Wnt signalling pathway’s role in primordial follicle activation.

show abstract

“…Primordial follicle initial recruitment is different from cyclic recruitment (Kallen et al, 2018). It is generally believed that cyclic recruitment is regulated by gonadotropins, while initial recruitment is not regulated by gonadotropins (McGee and Hsueh, 2000;Bian et al, 2021). Primordial follicle initial recruitment is mainly regulated by the signals in the pre-granulosa cells and oocyte, as well as by conditions such as growth factors and stress in the primordial follicle microenvironment (Bian et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…It is generally believed that cyclic recruitment is regulated by gonadotropins, while initial recruitment is not regulated by gonadotropins (McGee and Hsueh, 2000;Bian et al, 2021). Primordial follicle initial recruitment is mainly regulated by the signals in the pre-granulosa cells and oocyte, as well as by conditions such as growth factors and stress in the primordial follicle microenvironment (Bian et al, 2021). After the primordial follicle is activated, the pre-granulosa cells gradually change from flat to wedge-shaped, then cuboidal, and later called granulosa cells.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of primordial follicle activation and new pregnancy opportunity for premature ovarian failure patients

Zhang

He²,

Zhang³

et al. 2023

Front. Physiol.

View full text Add to dashboard Cite

Primordial follicles are the starting point of follicular development and the basic functional unit of female reproduction. Primordial follicles are formed around birth, and most of the primordial follicles then enter a dormant state. Since primordial follicles are limited in number and can’t be renewed, dormant primordial follicles cannot be reversed once they enter the growing state. Thus, the orderly occurrence of primordial follicles selective activation directly affects the rate of follicle consumption and thus determines the length of female reproductive lifespan. Studies have found that appropriately inhibiting the activation rate of primordial follicles can effectively slow down the rate of follicle consumption, maintain fertility and delay ovarian aging. Based on the known mechanisms of primordial follicle activation, primordial follicle in vitro activation (IVA) technique has been clinically developed. IVA can help patients with premature ovarian failure, middle-aged infertile women, or infertile women due to gynecological surgery treatment to solve infertility problems. The study of the mechanism of selective activation of primordial follicles can contribute to the development of more efficient and safe IVA techniques. In this paper, recent mechanisms of primordial follicle activation and its clinical application are reviewed.

show abstract

Transcriptional changes of mouse ovary during follicle initial or cyclic recruitment mediated by extra hormone treatment

Cited by 9 publications

References 56 publications

Genetic and epigenetic differentiation in response to genomic selection for avian lay date

Genetic and epigenetic differentiation in response to genomic selection for avian lay date

Transcriptomic profiling of neonatal mouse granulosa cells reveals new insights into primordial follicle activation

Mechanisms of primordial follicle activation and new pregnancy opportunity for premature ovarian failure patients

Contact Info

Product

Resources

About