Transcriptional changes revealed water acidification leads to the immune response and ovary maturation delay in the Chinese mitten crab Eriocheir sinensis

Luo, Bi-Yun; Qian, Hongli; Jiang, Hong; Xiong, Xin-Yi; Ye, Baoqing; Li, Xue; Guo, Ziqi; Knepper, M. A.

doi:10.1016/j.cbd.2021.100868

Cited by 12 publications

(9 citation statements)

References 47 publications

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“…Meanwhile, 27,975 unigenes (41.61%) were annotated in at least one database (Table S3, ESM 3). This annotation rate was similar to that of previously reported transcriptomes (Cheng et al, 2020; Yang et al, 2021) and higher than that reported in other crustacean species, such as Portunus trituberculatus (Liu et al, 2019a), Eriocheir sinensis (Luo et al, 2021a), and Macrobrachium rosenbergii (Jiang et al, 2019). The number of successfully annotated unigenes was 17,821 in NR (26.50%), 7268 in NT (10.81%), 7192 in KO (10.69%), 11,127 in SwissProt (16.55%), 20,592 in PFAM (30.62%), 20,589 in GO (30.62%), and 6174 in KOG (9.18%) (ESM 3).…”

Section: Resultssupporting

confidence: 90%

Comparative transcriptomic characterization of the ovary in the spawning process of the mud crab Scylla paramamosain

Tu,

Yu,

et al. 2024

Dev Growth Differ

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Oviposition is induced upon mating in most insects. Spawning is a physiological process that is fundamental for the reproduction of Scylla paramamosain. However, the molecular mechanisms underlying the spawning process in this species are poorly understood. Herein, comprehensive ovary transcriptomic analysis was conducted at the germinal vesicle breakdown stage (GVBD), spawning stage, 0.5 h post‐spawning stage, and 24 h post‐spawning stage of S. paramamosain for gene discovery. A total of 67,230 unigenes were generated, and 27,975 (41.61%) unigenes were annotated. Meanwhile, the differentially expressed genes (DEGs) between the different groups were identified, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was subsequently conducted. These results suggested that octopamine (OA) and tyramine (TA) could induce oviposition, while dopamine (DA) and serotonin (5‐hydroxytryptamine [5‐HT]) inhibit oviposition. The 20‐hydroxyecdysone (20E) and methyl farnesoate (MF) signal pathways might be positively associated with oviposition. Furthermore, numerous transcripts that encode neuropeptides and their G‐protein‐coupled receptors (GPCRs), such as CNMamide, RYamide, ecdysis‐triggering hormone (ETH), GPA2/GPB5 receptor, and Moody receptor, appear to be differentially expressed during the spawning process. Eleven unigenes were selected for qRT‐PCR and the pattern was found to be consistent with the transcriptome expression pattern. Our work is the first spawning‐related investigation of S. paramamosain focusing on the ovary at the whole transcriptome level. These findings assist in improving our understanding of spawning regulation in S. paramamosain and provide information for oviposition studies in other crustaceans.

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Section: Resultssupporting

confidence: 90%

Comparative transcriptomic characterization of the ovary in the spawning process of the mud crab Scylla paramamosain

Tu,

Yu,

et al. 2024

Dev Growth Differ

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“…Lysosomes are related to the hydrolysis of vitellogenin and energy demand during Macrobrachium nipponense ovarian maturation [41]. Lysosomal enzymes, especially cathepsin B and L, are associated with ovarian development in crustaceans [42,43]. We also found that cathepsin B and L in the ovaries of E. carinicauda were significantly up−regulated in response to carbonate alkalinity stress, which was consistent with the above results.…”

Section: Discussionsupporting

confidence: 90%

Effects of Long-Term High Carbonate Alkalinity Stress on the Ovarian Development in Exopalaemon carinicauda

Zhang

Wang

et al. 2022

Water

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Saline–alkaline water limits the growth and survival of aquatic animals due to its high carbonate alkalinity, high pH, and various ion imbalances. The ridgetail white prawn Exopalaemon carinicauda is strongly adaptable to the saline–alkaline water, making it an excellent candidate species for large-scale aquaculture in saline–alkaline areas. To explore the effect of long-term high carbonate alkalinity stress on ovarian development in E. carinicauda for assisting the development of saline–alkaline aquaculture, we performed ovary histology analysis and RNA–sequencing of the eyestalk and ovary in order to compare the transcriptomic responses of individuals in high carbonate alkalinity (8 mmol/L) with a control group (2 mmol/L) for 60 days. It was found that high carbonate alkalinity stress resulted in a loose arrangement of oogonia and a small number of surrounding follicular cells. A total of 1102 differentially expressed genes (DEGs) in ovary tissue were identified under high carbonate alkalinity stress, and the 18 important DEGs were associated with ovarian development. The majority of the DEGs were enriched in ECM–receptor interaction, Folate biosynthesis, the FoxO signaling pathway, insect hormone biosynthesis, and lysosome, which were involved in the ovarian development of E. carinicauda. A total of 468 DEGs were identified in eyestalk tissue under high carbonate alkalinity stress, and the 13 important DEGs were associated with ovarian development. KEGG enrichment analysis found that ECM–receptor interaction, folate biosynthesis, lysosome, metabolic pathways, and retinol metabolism may be involved in the ovarian development under high carbonate alkalinity stress. Our results provide new insights and reveal the genes and pathways involved in the ovarian development of E. carinicauda under long-term high carbonate alkalinity stress.

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“…In Tanner crabs, exposure to high pCO 2 during oogenesis had substantial negative carryover effect on both embryos [12] and the subsequent larvae [47]. High pCO 2 causes changes to gene expression and disrupts oocyte development in Chinese mitten crabs, Eriocheir sinensis suggesting direct effects on oogenesis could be a mechanism for these carryover effect [60]. Alternatively, high pCO 2 could simply increase the energy requirement to maintain homeostasis and thus decrease the energy available for other processes [61] including reproduction.…”

Section: Discussionmentioning

confidence: 99%

“…Eriocheir sinensis suggesting direct effects on oogenesis could be a mechanism for these carryover effect [60]. Alternatively, high pCO2 could simply increase the energy requirement to maintain homeostasis and thus decrease the energy available for other processes [61] including reproduction.…”

Section: Discussionmentioning

confidence: 99%

Effects of high pCO2 on snow crab embryos: Ocean acidification does not affect embryo development or larval hatching

Long

Swiney

Foy

2022

Preprint

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Ocean acidification, a decrease in ocean pH due to absorption of anthropogenic CO2, has variable effects on different species. To examine the effects of decreased pH on snow crab (Chionoecetes opilio), a commercial species in Alaska, we reared ovigerous females in one of three treatments: ambient pH (~8.1), pH 7.8, and pH 7.5, through two annual reproductive cycles. Morphometric changes during development and hatching success were measured for embryos both years and calcification was measured for the adult females at the end of the 2-year experiment. Embryos and larvae analyzed in year one were from oocytes developed, fertilized, and extruded in situ, whereas embryos and larvae in year two were from oocytes developed, fertilized, and extruded under acidified conditions in the laboratory. Embryo morphology during development was unaffected by pH during both years. The number of successfully hatched live larvae was unaffected by pH treatment in both years. Embryo mortality was very low, hatching success high, and neither differed with treatment in either year. Percent calcium in adult females' carapaces did not differ among treatments at the end of the experiment. The results from this two-year study suggest that snow crabs are well adapted to projected ocean pH levels within the next 2 centuries, although other life-history stages still need to be examined for sensitivity.

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Transcriptional changes revealed water acidification leads to the immune response and ovary maturation delay in the Chinese mitten crab Eriocheir sinensis

Cited by 12 publications

References 47 publications

Comparative transcriptomic characterization of the ovary in the spawning process of the mud crab Scylla paramamosain

Comparative transcriptomic characterization of the ovary in the spawning process of the mud crab Scylla paramamosain

Effects of Long-Term High Carbonate Alkalinity Stress on the Ovarian Development in Exopalaemon carinicauda

Effects of high pCO2 on snow crab embryos: Ocean acidification does not affect embryo development or larval hatching

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