Transcriptional Complementarity in Breast Cancer: Application to Detection of Circulating Tumor Cells

Houghton, Raymond L.; Dillon, Davin C.; Molesh, David A.; Zehentner, Barbara K.; Xu, Jiangchun; JIANG, JOHN; SCHMIDT, CHERYL; FRUDAKIS, ANTHONY; Repasky, Elizabeth A.; Filho, Aristides Maltez; Nolasco, Marcos; Badaró, Roberto; ZHANG, XINQUN; Roche, Patrick C.; Persing, David H.; Reed, Steven G.

doi:10.2165/00066982-200106020-00003

Cited by 42 publications

(15 citation statements)

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“…Furthermore, the suppression subtractive hybridization technology utilizes a normalization step that enables the recovery of not only abundant genes but also less abundant genes from the subtracted library. For instance, mammaglobin is a very abundant gene in breast tumors as demonstrated by Northern (Watson et al, 1999), microarray, and quantitative RT ± PCR (Houghton et al, 2001). This gene has been identi®ed by PCR-based subtraction, di erential display PCR, and conventional subtraction.…”

Section: Discussionmentioning

confidence: 99%

“…It is evident from the microarray pro®le that these genes are overexpressed in breast tumors and/or normal breast tissues compared to other normal tissues. Another characteristic is that while each of the four genes is expressed in a di erent subset of the breast tumor panel, there is complementation such that, taken together, expression of these four genes shows threefold upregulation in 64% (18 out of 27) of the breast tumor samples (Houghton et al, 2001).…”

Section: Identification Of Cdnas Differentially Expressed In Breast Tmentioning

confidence: 99%

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Discovery of differentially expressed genes in human breast cancer using subtracted cDNA libraries and cDNA microarrays

et al. 2002

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Identifying novel and known genes that are di erentially expressed in breast cancer has important implications in understanding the biology of breast tumorigenesis and developing new diagnostic and therapeutic agents. In this study we have combined two powerful technologies, PCRbased cDNA subtraction and cDNA microarray, as a high throughput methodology designed to identify cDNA clones that are breast tumor-and tissue-speci®c and are overexpressed in breast tumors. Approximately 2000 cDNA clones generated from the subtracted breast tumor library were arrayed on the microarray chips. The arrayed target cDNAs were then hybridized with 30 pairs of¯uorescent-labeled cDNA probes generated from breast tumors and normal tissues to determine the tissue distribution and tumor speci®city. cDNA clones showing overexpression in breast tumors by microarray were further analysed by DNA sequencing, GenBank and EST database searches, and quantitative real time PCR. We identi®ed several known genes, including mammaglobin, cytokeratin 19, ®bronectin, and hair-speci®c type II keratin, which have previously been shown to be overexpressed in breast tumors and may play an important role in the malignance of breast. We also discovered B726P which appears to be an isoform of NY-BR-1, a breast tissue-speci®c gene. Two additional clones discovered, B709P and GABA A receptor p subunit, were not previously described for their overexpression pro®le in breast tumors. Thus, combining PCRbased cDNA subtraction and cDNA microarray allowed for an e cient way to identify and validate genes with elevated mRNA expression levels in breast cancer that may potentially be involved in breast cancer progression. These di erentially expressed genes may be of potential utility as therapeutic and diagnostic targets for breast cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Identification Of Cdnas Differentially Expressed In Breast Tmentioning

confidence: 99%

Discovery of differentially expressed genes in human breast cancer using subtracted cDNA libraries and cDNA microarrays

et al. 2002

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“…AF414087] was identified by Miksicek et al (2002) using the cDNA xProfiler tool. SBEM product is similar to proteins B511 s (Houghton et al 2001) and BS106 (Colpitts et al 2002). The SBEM gene is predicted to code for a low molecular weight glycoprotein with highly similarity to sialomucins, including MUC1.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic accuracy of small breast epithelial mucin mRNA as a marker for bone marrow micrometastasis in breast cancer: a pilot study

Valladares‐Ayerbes

Iglesias-Díaz

Díaz‐Prado

et al. 2009

J Cancer Res Clin Oncol

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“…Potential for this type of diagnosis has been demonstrated for the mammaglobin gene in metastatic breast cancer (Houghton et al, 2001;Watson et al, 1999).…”

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confidence: 99%

Characterization of KLK4 expression and detection of KLK4-specific antibody in prostate cancer patient sera

et al. 2002

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Transcriptional Complementarity in Breast Cancer: Application to Detection of Circulating Tumor Cells

Cited by 42 publications

References 0 publications

Discovery of differentially expressed genes in human breast cancer using subtracted cDNA libraries and cDNA microarrays

Discovery of differentially expressed genes in human breast cancer using subtracted cDNA libraries and cDNA microarrays

Diagnostic accuracy of small breast epithelial mucin mRNA as a marker for bone marrow micrometastasis in breast cancer: a pilot study

Characterization of KLK4 expression and detection of KLK4-specific antibody in prostate cancer patient sera

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