Transcriptional diversity of DMRT1 (dsx- and mab3-related transcription factor 1) in human testis

Cheng, Han; Ying, Ming; Tian, Yi Hao; Guo, Yiqing; McElreavey, Ken; Zhou, Rongjia

doi:10.1038/sj.cr.7310050

Cited by 23 publications

(16 citation statements)

References 26 publications

(25 reference statements)

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“…Current knowledge concerning the function of this gene for the moment does not reveal the significance of this expression pattern. Very recent studies on the human testis have evidenced that three different transcripts may be generated by alternative splicing for this gene, and that they show different expression levels in the testis (Cheng et al, 2006). These data probably reflect that regulation of DMRT1 expression is controlled by a complex mechanism and that different transcript isoforms may play different roles in gonadal development and function.…”

Section: Discussionmentioning

confidence: 90%

Meiosis Onset Is Postponed to Postnatal Stages during Ovotestis Development in Female Moles

Zurita

Carmona

Lupiáñez

et al. 2006

Sex Dev

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In mammals, germ cells are important both during development and for the function of female gonads, whereas male gonads may develop in the absence of germ cells. The gonads of female moles (genus Talpa) develop according to a testis-like pattern which results in the formation of ovotestes. In this paper, we studied the expression pattern of several pre-meiotic and meiotic germ cell markers, in order to establish the precise time of meiosis onset in the mole species T. occidentalis, and to investigate the location and possible role of germ cells in ovotestis organogenesis. Our results evidenced that: (1) the asymmetrical distribution of primordial germ cells, which concentrate in the cortex of the XX gonad, is brought about by germ cell depletion from the medulla between the s5a and s5b stages, (2) XX germ cells enter meiosis postnatally, which is quite exceptional among eutherian mammals, and (3) XX but not XY germ cells of moles express DMRT1 during premeiotic stages of development, an expression pattern not described previously in vertebrates.

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Section: Discussionmentioning

confidence: 90%

Meiosis Onset Is Postponed to Postnatal Stages during Ovotestis Development in Female Moles

Zurita

Carmona

Lupiáñez

et al. 2006

Sex Dev

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“…Here we have further shown that the DMRT1 genes have another characteristic conserved among phyla: a special alternative splicing. The alternative splicing events occurred 3′ to the DM domain of the diverse species, including Drosophila (dsx) (Burtis and Baker 1989), zebrafish (dmrt1) , the rice field eel (dmrt1) , chicken (DMRT1), and human (DMRT1) (Cheng et al 2006). Although alternative splicing is known to increase diversity of the proteomic world, evolutionary and functional significance for the vast majority of alternative splicing events is unknown (Kriventseva et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Multiple alternative splicing in gonads of chicken DMRT1

Zhao

et al. 2006

Dev Genes Evol

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Many basic cellular processes are shared across vast phylogenetic distances, whereas sex-determining mechanisms are highly variable between phyla, although the existence of two sexes is nearly universal in the animal kingdom. However, the evolutionarily conserved DMRT1/dsx/mab3 with a common zinc finger-like DNA-binding motif, DM domain, share both similar structure and function between phyla. Here we report that six transcripts of the chicken DMRT1 were generated in gonads by multiple alternative splicing. By cDNA cloning and genomic structure analysis, we found that there were nine exons of DMRT1, which were involved in alternatively splicing to generate the DMRT1 transcripts. Northern blotting and reverse transcription (RT) PCR analysis revealed that the expression of chicken DMRT1 was testis-specific in adults. Whole-mount in situ hybridizations and RT-PCR indicated that DMRT1 b was specially expressed in embryo gonads and higher in male than female gonads at stage 31. The female gonad had stronger DMRT1 c expression than the male one, whereas DMRT1 f was detectable only in the male gonad at stage 31 of the key time of sex gonadal differentiation. The differential expression of these transcripts during gonadal differentiation provides new insight into roles of alternative splicing of DMRT1 in governing sex differentiation of the chicken.

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“…In the case of DMRT1 , alternative splicing may also play a role in defining its function. DMRT1 is alternatively spliced in human testis (Cheng et al, 2006) and in the gonads of lower vertebrates (Sreenivasulu et al, 2002;Guo et al, 2005). Most recently this has also been shown for DMRT1 transcripts in embryonic chicken gonads, with a male specific splice variant detected at stage 31, the time of gonadal sex differentiation (Zhao et al, 2007).…”

Section: When Are Sex-determining Genes Operating?mentioning

confidence: 97%

Avian sex determination: what, when and where?

Smith

Roeszler

Hudson

et al. 2007

Cytogenet Genome Res

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Sex is determined genetically in all birds, but the underlying mechanism remains unknown. All species have a ZZ/ZW sex chromosome system characterised by female (ZW) heterogamety, but the chromosomes themselves can be heteromorphic (in most birds) or homomorphic (in the flightless ratites). Sex in birds might be determined by the dosage of a Z-linked gene (two in males, one in females) or by a dominant ovary-determining gene carried on the W sex chromosome, or both. Sex chromosome aneuploidy has not been conclusively documented in birds to differentiate between these possibilities. By definition, the sex chromosomes of birds must carry one or more sex-determining genes. In this review of avian sex determination, we ask what, when and where? What is the nature of the avian sex determinant? When should it be expressed in the developing embryo, and where is it expressed? The last two questions arise due to evidence suggesting that sex-determining genes in birds might be operating prior to overt sexual differentiation of the gonads into testes or ovaries, and in tissues other than the urogenital system.

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Transcriptional diversity of DMRT1 (dsx- and mab3-related transcription factor 1) in human testis

Cited by 23 publications

References 26 publications

Meiosis Onset Is Postponed to Postnatal Stages during Ovotestis Development in Female Moles

Meiosis Onset Is Postponed to Postnatal Stages during Ovotestis Development in Female Moles

Multiple alternative splicing in gonads of chicken DMRT1

Avian sex determination: what, when and where?

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