Transcriptional effect of a calmodulin inhibitor, W-7, on the ligninolytic enzyme genes in Phanerochaete chrysosporium

Sakamoto, Takako; Kitaura, Hironori; Minami, Masahiko; Honda, Yoshio; Watanabe, Takashi; Ueda, Akio; Suzuki, Kaoru; Irie, Toshikazu

doi:10.1007/s00294-010-0309-z

Cited by 13 publications

(18 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, these results were inconclusive because Mn 2+ -dependent oxidation activity, which represents the total activity of MnP and VP, was the same in both strains (Yao et al 2013). In P. chrysosporium, our previous studies suggested that the CaM gene was induced by an increase of intracellular cAMP concentration (Minami et al 2007(Minami et al , 2009Sakamoto et al 2010Sakamoto et al , 2012 and that CaM positively regulates the expression of almost all genes encoding isozymes of LiP and MnP (Sakamoto et al 2010(Sakamoto et al , 2012. Because the present study suggested that cAMP also induces transcription of the CaM gene in P. ostreatus, similar to P. chrysosporium (Fig.…”

Section: Discussionsupporting

confidence: 68%

“…2). These results suggest that CaM negatively regulates MnP and VP activities in P. ostreatus, in contrast to the positive effect of CaM on MnP and LiP in P. chrysosporium (Sakamoto et al 2010). …”

Section: Effect Of Cam Inhibitors On Mnp Activitycontrasting

confidence: 56%

“…In our previous study, we found that CaM gene expression is regulated in parallel with genes encoding most isozymes of LiP and MnP in P. chrysosporium (Minami et al 2007(Minami et al , 2009. Furthermore, the addition of the CaM inhibitor W7 reduces the amount of LiP and MnP isozyme transcripts, leading to reduced activity of these enzymes (Sakamoto et al 2010(Sakamoto et al , 2012. Together, these data suggest that CaM positively regulates the transcription of LMEs in P. chrysosporium.…”

Section: Introductionmentioning

confidence: 84%

See 2 more Smart Citations

Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus

et al. 2014

Self Cite

View full text Add to dashboard Cite

Previously, we suppressed the expression of genes encoding isozymes of lignin peroxidase (LiP) and manganese peroxidase (MnP) using a calmodulin (CaM) inhibitor, W7, in the white-rot fungus Phanerochaete chrysosporium; this suggested that CaM positively regulates their expression. Here, we studied the role of CaM in another white-rot fungus, Pleurotus ostreatus, which produces MnP and versatile peroxidase (VP), but not LiP. W7 upregulated Mn(2+)-dependent oxidation of guaiacol, suggesting that CaM negatively regulates the production of the enzymes. Suppression of CaM in P. ostreatus using RNAi also led to upregulation of enzyme activity, whereas overexpression of CaM in P. ostreatus caused downregulation. Real-time RT-PCR showed that MnP1-6 and VP3 levels in the CaM-knockdown strain were higher than those in the wild-type strain, while MnP-5 and -6 and VP1 and 2 levels in the CaM-overexpressing strain were lower than in the wild type. Moreover, we also found that another ligninolytic enzyme, laccase, which is not produced by P. chrysosporium, was negatively regulated by CaM in P. ostreatus similar to MnP and VP. Although overexpression of CaM did not reduce the ability of P. ostreatus to digest beech wood powder, the percentage of lignin remaining in the digest was slightly higher than in the wild-type strain digest.

show abstract

Section: Discussionsupporting

confidence: 68%

Section: Effect Of Cam Inhibitors On Mnp Activitycontrasting

confidence: 56%

Section: Introductionmentioning

confidence: 84%

See 1 more Smart Citation

Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…The CaM gene ( cam ) is present as a single isoform in the P. chrysosporium genome (Martinez et al 2004). We previously revealed that the CaM pathway is required for expression of lip and mnp genes in P. chrysosporium (Minami et al 2007; Minami et al 2009; Sakamoto et al 2010), but the relationship between these signaling factors that leads to LiP and MnP expression has remained unclear. Here, we report experimental results suggesting that CaM expression is regulated by the cAMP pathway, and that cAMP controls LiP and MnP expression mainly through regulation of CaM expression.…”

Section: Introductionmentioning

confidence: 99%

A calmodulin inhibitor, W-7 influences the effect of cyclic adenosine 3', 5'-monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium

et al. 2012

Self Cite

View full text Add to dashboard Cite

The capacity of white-rot fungi to degrade wood lignin may be highly applicable to the development of novel bioreactor systems, but the mechanisms underlying this function are not yet fully understood. Lignin peroxidase (LiP) and manganese peroxidase (MnP), which are thought to be very important for the ligninolytic property, demonstrated increased activity in Phanerochaete chrysosporium RP-78 (FGSC #9002, ATCC MYA-4764™) cultures following exposure to 5 mM cyclic adenosine 3', 5'-monophosphate (cAMP) and 500 μM 3'-isobutyl-1-methylxanthine (IBMX), a phosphodiesterase inhibitor. Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that transcription of most LiP and MnP isozyme genes was statistically significantly upregulated in the presence of the cAMP and IBMX compared to the untreated condition. However, 100 μM calmodulin (CaM) inhibitor N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), which had insignificant effects on fungal growth and intracellular cAMP concentration, not only offset the increased activity and transcription induced by the drugs, but also decreased them to below basal levels. Like the isozyme genes, transcription of the CaM gene (cam) was also upregulated by cAMP and IBMX. These results suggest that cAMP signaling functions to increase the transcription of LiP and MnP through the induction of cam transcription.

show abstract

“…These expression profiles were similar to those of cultures with added atropine. It was hypothesized that atropine inhibits CaM signaling, thereby inhibiting cAMP signaling, or vice versa, to affect LiP and MnP production (Sakamoto et al 2010) (Fig. 1).…”

Section: Differential Gene Expression In Phanerochaete Species Grown mentioning

confidence: 99%

Expression and regulation of genes encoding lignocellulose-degrading activity in the genus Phanerochaete

MacDonald

Suzuki

Master

2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

As white-rot basidiomycetes, Phanerochaete species are critical to the cycling of carbon sequestered as woody biomass, and are predicted to encode many enzymes that can be harnessed to promote the conversion of lignocellulose to sugars for fermentation to fuels and chemicals. Advances in genomic, transcriptomic, and proteomic technologies have enabled detailed analyses of different Phanerochaete species and have revealed numerous enzyme families required for lignocellulose utilization, as well as insight into the regulation of corresponding genes. Recent studies of Phanerochaete are also exemplified by molecular analyses following cultivation on different wood preparations, and show substrate-dependent responses that were difficult to predict using model compounds or isolated plant polysaccharides. The aim of this mini-review is to synthesize results from studies that have applied recent advances in molecular tools to evaluate the expression and regulation of proteins that contribute to lignocellulose conversion in Phanerochaete species. The identification of proteins with as yet unknown function are also highlighted and noted as important targets for future investigation of white-rot decay.

show abstract

Transcriptional effect of a calmodulin inhibitor, W-7, on the ligninolytic enzyme genes in Phanerochaete chrysosporium

Cited by 13 publications

References 51 publications

Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus

Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus

A calmodulin inhibitor, W-7 influences the effect of cyclic adenosine 3', 5'-monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium

Expression and regulation of genes encoding lignocellulose-degrading activity in the genus Phanerochaete

Contact Info

Product

Resources

About