Transcriptional expression analysis of ABC efflux transporters and xenobiotic-metabolizing enzymes in the Chinese rare minnow

Yuan, Lilai; Lv, Biping; Zha, Jinmiao; Wang, Zijian

doi:10.1016/j.etap.2014.03.015

Cited by 14 publications

(7 citation statements)

References 57 publications

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“…Also the mRNA of abcb1a showed a rise in the abdominal viscera. All this is in agreement with data from abcg2, abcc2 and abcb1a transcripts found in gonads, intestine, liver and kidneys of juvenile and adult fish (Lončar et al, 2010;Tutundjian et al, 2002;Yuan et al, 2014). The change from the yolk sac epithelium as prominent organ of ABC-mediated transport to the abdominal viscera reflects the transition from the yolk-dependent early developmental stage to stages with a more adult-type anatomy and physiology.…”

Section: Abc Transporters In Developing (1-20 Dph) O Mykisssupporting

confidence: 90%

ABC transporters and xenobiotic defense systems in early life stages of rainbow trout (Oncorhynchus mykiss)

Kropf

Segner

Fent

2016

Comparative Biochemistry and Physiology Part C: Toxicology &

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Embryos of oviparous fish, in contrast to (ovo) viviparous species, develop in the aquatic environment, and therefore need solute transport systems at their body surfaces for maintaining internal homeostasis and defending against potentially harmful substances. We hypothesized that solute transporters undergo changes in tissue distribution from the embryo to the larval stage. We therefore studied the mRNA profiles of eight ABC transporters (abcb1a, abcb1b, abcc1, abcc2, abcc3, abcc4, abcc5, abcg2) and three solute carriers (oatp1d, putative oatp2 putative, mate1) in different body regions (head, yolk sac epithelium, abdominal viscera, skin/muscles) of developing rainbow trout. Additionally, we investigated mRNA levels of phase I (cyp1a, cyp3a) and phase II (gstp, putative ugt1, putative ugt2) biotransformation enzymes. The study covered the developmental period from the eleuthero-embryo stage to the first-feeding larval stage (1-20days post-hatch, dph). At 1dph, transcripts of abcc2, abcc4, abcg2, cyp3a, gstp, putative mate1, and putative oatp2 occurred primarily in the yolk sac epithelium, whereas at later stages expression of these genes was predominantly observed in the abdominal viscera. The functional activity of ABC transporters in fish early life stages was assessed by rhodamine B accumulation assays. Finally, we investigated the potential impact of xenobiotics (clotrimazole, clofibric acid) on the ABC and biotransformation systems of trout early life stages. While clofibric acid had no effect, clotrimazole lead to an increased rhodamine B accumulation. The results provide evidence that the transition from the eleuthero-embryo to the larval stage is accompanied by a major alteration in tissue expression of ABC transporters.

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Section: Abc Transporters In Developing (1-20 Dph) O Mykisssupporting

confidence: 90%

ABC transporters and xenobiotic defense systems in early life stages of rainbow trout (Oncorhynchus mykiss)

Kropf

Segner

Fent

2016

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…The importance of linking all four biotransformation phases (0, I, II and III) in liver of fish with the aim to identify common pathways of action of known and emerging aquatic pollutants as nanoparticles is confirmed by the increasing number of papers focusing on transcriptomic profile (Paetzold et al, 2009;Zucchi et al, 2010;Costa et al, 2013;Yuan et al, 2014).…”

Section: Introductionmentioning

confidence: 92%

“…Also the multixenobiotic resistance mechanism (MXR) involved in phase 0 and III of biotransformation (MXR) has been deeply characterized in aquatic organism and fish species (Kurelec, 1992;Leslie et al, 2005;Xu et al, 2005). Enhanced expression of ABC genes has been more recently suggested to confer resistance against organic and inorganic toxicants in aquatic organisms (Miller et al, 2007;Zucchi et al, 2010;Della Torre et al, 2012;2014a;2014b;Yuan et al 2014;Luckenbach et al, 2014). The current knowledge about ABC proteins in teleost fish is still limited, although similarities in tissue distribution between teleosts and mammals have been recently identified confirming the role of the most toxicological relevant ABC transporters of the three ABC subfamilies (Abcb, Abcc, Abcg) in the biochemical defense against toxicants (Luckenbach et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Combination effects of nano-TiO2 and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on biotransformation gene expression in the liver of European sea bass Dicentrarchus labrax

Vannuccini

Grassi

Leaver

et al. 2015

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…In contrast, cyp1b1 and cyp1c1 showed the highest induction at 48 hpf from the 100 mg/L BaP treatments (5.7-and 20-fold, respectively), relative to the same time point control (Figs. 4 and 5), and the induced pattern of these two genes The sequence information and the primer pairs used in the study was according to a previous study of our laboratory (Yuan et al, 2014). was similar over time during the exposure (Fig. 5).…”

Section: Transcriptional Effects Of Xenobiotic Enzymes/transporters Fmentioning

confidence: 99%

Basal and benzo[a]pyrene-induced expression profile of phase I and II enzymes and ABC transporter mRNA in the early life stage of Chinese rare minnows (Gobiocypris rarus)

Yuan

Zha

et al. 2014

Ecotoxicology and Environmental Safety

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a b s t r a c t ATP-binding cassette (ABC) transporters together with phase I and II detoxification enzymes have been considered as included in a cellular detoxification system. Previous studies have highlighted the involvement of aryl hydrocarbon receptor (AHR) and Cyp1a in PAH-induced embryo toxicity. However, the response of other xenobiotic enzymes/transporters in PAH-mediated embryo toxicity is not fully characterized. In the present study, rare minnow embryos were exposed to 10 and 100 mg/L BaP within 4 h post-fertilization (hpf) up to 168 hpf. RNA was extracted at 24, 48, 96, and 168 hpf. The basal and BaP-induced expression of phase I enzyme genes (cyp1a, 1b1, and 1c1), phase II enzyme gene (gstm and ugt1a), and ABC transporter genes (abcb1, abcc1, abcc2, and abcg2) mRNA was determined using realtime PCR. Severe developmental defects (e.g., spinal deformities, pericardial and yolk-sac edema) were observed in the BaP treated groups. The basal expression showed that gstm was most strongly expressed, followed by abcb1, ugt1a, and abcc2, whereas cyp1a, 1b1, 1c1, and abcg2 showed weak expression. BaP significantly induced the mRNA expression of three CYP1s (cyp1a, 1b1, and 1c1) (p o0.05) and the ABC transporters (abcc1, abcc2, and abcg2) in a dose-dependent manner. However, the mRNA expression of Phase II enzymes (gstm, ugt1a) for the BaP treatments showed no significant difference with that of the controls. Furthermore, distinct induced patterns of these genes were observed during different exposure periods. Simultaneous up-regulation of the cyp and ABC transporter gene transcripts suggests that a possible involvement and cooperation in the detoxification process could provide protection against the BaP toxicity of rare minnows at the early life stage.

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Transcriptional expression analysis of ABC efflux transporters and xenobiotic-metabolizing enzymes in the Chinese rare minnow

Cited by 14 publications

References 57 publications

ABC transporters and xenobiotic defense systems in early life stages of rainbow trout (Oncorhynchus mykiss)

ABC transporters and xenobiotic defense systems in early life stages of rainbow trout (Oncorhynchus mykiss)

Combination effects of nano-TiO2 and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on biotransformation gene expression in the liver of European sea bass Dicentrarchus labrax

Basal and benzo[a]pyrene-induced expression profile of phase I and II enzymes and ABC transporter mRNA in the early life stage of Chinese rare minnows (Gobiocypris rarus)

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