Transcriptional expression of survivin and its splice variants in cervical carcinomas

Abstract

The objective of this study was to evaluate transcriptional expression of survivin and the two splice variants (survivin-2B and survivin-DeltaEx3) in cervical carcinomas. The gene expression levels of survivin and its splice variants in 11 human cervical carcinoma cell lines and 20 malignant and 12 normal cervical tissue samples were analyzed using quantitative reverse transcription-polymerase chain reaction analysis. Gene expression levels of survivin and survivin-DeltaEx3 in cell lines were higher than those in normal cervical tissues (P= 0.0193 and 0.0489). Transcript levels of survivin and survivin-DeltaEx3 in carcinoma tissues were also higher than those in normal controls (P= 0.0016 and 0.0011). Gene expression levels of survivin and survivin-DeltaEx3 in adenocarcinomas were statistically higher than those in squamous cell carcinomas (P= 0.0260 and 0.0487). There was no significant difference in survivin-2B gene expression between malignant and normal cervical samples or different histologic types. The ratios of survivin-2B/survivin and survivin-DeltaEx3/survivin in carcinoma tissues were higher than those in normal controls (P= 0.0288 and 0.0081). Interestingly, the ratio of survivin-2B/survivin was increased in the patients with higher stages and with pelvic lymph node metastasis (P= 0.0205 and 0.0437), respectively. We conclude that survivin and its splice variants might be involved in the pathogenesis and development of cervical carcinomas.

Gene expression analysis of interferon κ in laser capture microdissected cervical epithelium

“…Furthermore, many published techniques claiming high-quality RNA isolation from FR tissue either relied only on expression of HKGs [2,3] and A 260 /A 280 ratios [34,35] or did not perform RNA quality assessment at all [8,10,36]. Here we show that contrary to previous results with FR tissue [4,[37][38][39], the modified Arcturus RNA extraction method was optimal for the extraction of high-quality RNA compared with the Ambion and Sigma methods for cervical biopsy sections (Fig.…”

“…The literature suggests that frozen (FR) tissue is optimal for the extraction of high-quality RNA [2,3]; however, this has not been shown for FR cervical tissue. Numerous techniques and commercially available kits exist for RNA extraction from FR tissue [4][5][6][7], and some have been used for cervical specimen extraction [8][9][10]. However, the integrity of the RNA and the reproducibility of the techniques are often overlooked.…”

Clinical and immunological evaluation of anti-apoptosis protein, survivin-derived peptide vaccine in phase I clinical study for patients with advanced or recurrent breast cancer

“…There is also a report that survivin-2B was dominantly expressed in gastric cancer [ 37 ]. Futakuchi et al [ 38 ] reported that the ratios of survivin-2B/survivin and survivin-ΔEx3/survivin in malignant cervical tissue samples were significantly higher than those in normal cervical tissue templates. Moreover, the ratio of survivin-2B/survivin was increased in patients with higher stages and with pelvic lymph node metastasis.…”

Survivin isoform expression in arsenic trioxide‐treated acute promyelocytic leukemia cell line and patients: The odd expression pattern of survivin‐2α

“…). It has been reported that the ratios of variants/survivin have some indication in clinico‐pathological relevance: an association with prognosis, drug resistance, grade of cancer and carcinoma tissues toward normal controls . We did not see any significant differences in the variants/survivin ratios between the normal, diagnoses and relapse groups (data not shown).…”