Transcriptional profiling in response to terminal drought stress reveals differential responses along the wheat genome

Aprile, Alessio; Mastrangelo, Anna Maria; Leonardis, Anna Maria De; Galiba, Gábor; Roncaglia, Enrica; Ferrari, Francesco; Bellis, Luigi De; Turchi, Luana; Giuliano, Giovanni; Cattivelli, Luigi

doi:10.1186/1471-2164-10-279

Cited by 147 publications

(115 citation statements)

References 77 publications

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“…The "omics" technologies have provided novel opportunities and expectations for the identification of transcriptional, translational and post-translational mechanisms and signaling pathways that regulate the plant response(s) to abiotic stress including high temperature [88,89]. DNA is the starting point of all molecular evidences related to heat stress tolerance in plants and contains several heat stress responsive genes in their genome (genomics).…”

Section: "Omics" Technologiesmentioning

confidence: 99%

Heat Stress Responses and Thermotolerance

Hemantaranjan¹,

Bhanu²,

Singh³

et al. 2014

APAR

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climate change is that most agricultural regions will experience additional extreme environmental fluctuations [2]. Direct injuries due to high temperatures include protein denaturation and aggregation and increased fluidity of membrane lipids. Indirect or slower heat injuries include inactivation of enzymes in chloroplast and mitochondria, inhibition of protein synthesis, protein degradation and loss of membrane integrity [3]. Heat stress also affects the organization of microtubules by splitting and/or elongation of spindles, formation of microtubule asters in mitotic cells and elongation of phragmoplast microtubules [4].The unfavourable effects of heat stress can be mitigated by developing crop plants with improved thermotolerance using an assortment of genetic approaches. For this reason, a thorough understanding of physiological responses of plants to high temperature, mechanisms of heat tolerance and possible strategies for improving crop thermotolerance is crucial. Acquiring thermotolerance is a lively progression by which considerable amounts of plant resources are diverted to structural and functional maintenance to escape damages caused by heat stress. Although biochemical and molecular aspects of thermotolerance in plants are relatively well understood, additional studies focused on phenotypic flexibility and assimilate partitioning under heat stress and factors modulating crop heat tolerance are imperative. High temperature during seed germination may slow down or totally inhibit germination, depending on plant species and the intensity of the stress [5]. At later stages, high IntroductionFuture global climate change, with predicted 1.5-5.8 °C increases in temperatures by 2100 has to cause heat stress to create threats to agricultural production [1]. An increase in global temperature ranging from 1.1 to 6.4 °C depending on global emissions scenarios, will accompany the rises in atmospheric CO 2 . Though high temperature and other abiotic stresses are clearly limiting factors for crops cultivated on marginal lands, crop productivity far and wide is often at the mercy of random environmental fluctuations. Existing assumption about global Heat Stress Responses and Thermotolerance AbstractThe rising ambient temperature by plant cells is crucial for the timely activation of various molecular defences before the appearance of heat damage. The heat-threshold level varies considerably at different developmental stages. With a view to survive under heat stress, mechanisms of regulation at the molecular level enable plants to prosper. Traditional breeding contributed for improving heat tolerance meagrely. The genetic transformation approach needs to be accelerated that can mitigate harmful effects by developing improved thermotolerance of crop plants. In this background, a thorough understanding of physiological responses of plants to high temperature, mechanisms of heat tolerance and possible strategies is vital. Temperature changes are sensed through cellular responses due to signal transduction into the c...

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Section: "Omics" Technologiesmentioning

confidence: 99%

Heat Stress Responses and Thermotolerance

Hemantaranjan¹,

Bhanu²,

Singh³

et al. 2014

APAR

View full text Add to dashboard Cite

show abstract

“…Gene expression profiling of wheat subjected to biotic and abiotic stress has been conducted previously (Aprile et al, 2009;Ergen et al, 2009;Krugman et al, 2010;Mohammadi et al, 2007;Reddy et al, 2013b;Szucs et al, 2010;Xue et al, 2006). However, these studies were not conducted in widely planted commercial wheat cultivars with distinct adaptation mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Physiology and transcriptomics of water-deficit stress responses in wheat cultivars TAM 111 and TAM 112

Reddy

Liu

Rudd

et al. 2014

Journal of Plant Physiology

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Hard red winter wheat crops on the U.S. Southern Great Plains often experience moderate to severe drought stress, especially during the grain filling stage, resulting in significant yield losses. Cultivars TAM 111 and TAM 112 are widely cultivated in the region, share parentage and showed superior but distinct adaption mechanisms under water-deficit (WD) conditions. Nevertheless, the physiological and molecular basis of their adaptation remains unknown. A greenhouse study was conducted to understand

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“…To investigate the early effects of glyphosate and TdGST overexpression we performed transient transformation experiments in N. tabacum protoplasts using AleuGFP [6] or GFPChi [5] co-transformed with TdGST [7] or incubated with glyphosate.…”

Section: Discussionmentioning

confidence: 99%

“…We observed an adaptive reaction of tobacco protoplasts evidenced by alterations in the fluorescent labeling of the vacuolar complex. The reaction was triggered by the treatment with glyphosate but also by the overexpression of a Triticum durum TdGST gene [7]. The correlation of non-target-site glyphosate resistance mechanisms [8] with an independent regulation of cell compartmentalization will be discussed.…”

Section: Introductionmentioning

confidence: 99%

Vacuolar Sorting Mechanisms are Differently Influenced by Detoxification Processes

Barozzi

Sabella

Aprile

et al. 2015

Proceedings of 1st Electronic Conference on Molecular Science

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Abstract:Glyphosate is a non-selective herbicide that inhibits the shikimate pathway's enzyme EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) preventing the production of aromatic amino acids. This herbicide is largely used and appreciated because it controls a wide range of annual and perennial weeds but it has a minimal environmental impact when compared with other herbicides. Initially, it was thought that resistance to glyphosate was not easy to evolve but the continuous applications, as it happened for other herbicides, induced the development of several glyphosate-resistant weeds. Glyphosate resistance can be developed as target-site or non-target-site mechanisms. In the target-site mechanism of resistance, either a mutation on the EPSPS enzyme (enzyme modification) or the overexpression of the EPSPS enzyme was found to confer resistance. In the non-target-site mechanism of glyphosate resistance, the translocation and the neutralization of the herbicide is observed. Pumping glyphosate into vacuoles via membrane transporters has been suggested as a possible process involved in the restricted glyphosate translocation. As a consequence, a different vacuolar organization or plasticity could be an interesting character or marker to correlate to glyphosate resistance. Vacuolar markers AleuGFP (Sar1 dependent sorting) or GFPChi (Sar1 independent sorting) respectively can be used to monitor independent vacuolar sorting mechanisms during glyphosate-induced stress. We observed that the adaptive reaction of tobacco protoplasts vacuolar complex to the treatment with glyphosate could be mimicked by the overexpression of a Triticum durum TdGST gene. Previous analysis evidenced that the herbicide glyphosate increased TdGST expression, confirming the role of GST in the protection against xenobiotics. Non-target-site glyphosate resistance mechanisms may correlate with an independent regulation of cell OPEN ACCESS 2 compartmentalization and herbicide-induced genes may have a direct effect on it.

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Transcriptional profiling in response to terminal drought stress reveals differential responses along the wheat genome

Cited by 147 publications

References 77 publications

Heat Stress Responses and Thermotolerance

Heat Stress Responses and Thermotolerance

Physiology and transcriptomics of water-deficit stress responses in wheat cultivars TAM 111 and TAM 112

Vacuolar Sorting Mechanisms are Differently Influenced by Detoxification Processes

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