Transcriptional profiling of adult Drosophila antennae by high-throughput sequencing

Shiao, Meng Shin; Fan, Wen; Fang, Shu; Lu, Mei-Yeh Jade; Kondo, Rumi; Li, Wen-Hsiung

doi:10.1186/1810-522x-52-42

Cited by 30 publications

(36 citation statements)

References 32 publications

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“…The expression of such receptors is in accordance with results from other RNAseq studies of the D. melanogaster antenna. In those studies, multiple genes previously identified by in situ hybridization or through reporter gene analyses to be only expressed in larvae or the maxillary palps were also detected in adults [42,43]. The functional significance of this, if any, remains to be determined.…”

Section: (B) Population Differences In Electrophysiological Responsesmentioning

confidence: 99%

Population differences in olfaction accompany host shift in Drosophila mojavensis

et al. 2016

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Evolutionary shifts in plant -herbivore interactions provide a model for understanding the link among the evolution of behaviour, ecological specialization and incipient speciation. Drosophila mojavensis uses different host cacti across its range, and volatile chemicals emitted by the host are the primary cue for host plant identification. In this study, we show that changes in host plant use between distinct D. mojavensis populations are accompanied by changes in the olfactory system. Specifically, we observe differences in olfactory receptor neuron specificity and sensitivity, as well as changes in sensillar subtype abundance, between populations. Additionally, RNA-seq analyses reveal differential gene expression between populations for members of the odorant receptor gene family. Hence, alterations in host preference are associated with changes in development, regulation and function at the olfactory periphery.

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Section: (B) Population Differences In Electrophysiological Responsesmentioning

confidence: 99%

Population differences in olfaction accompany host shift in Drosophila mojavensis

et al. 2016

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“…Interestingly, MA‐biased genes included a relatively small number of chemosensory genes, but notably contain many immune‐related genes, including antimicrobial peptides such as attacin, defencin, cecropin, and diptricin (Figure 2c; see the full list in Table S6). Interestingly, D. melanogaster also upregulated for the immune‐related genes in MA, when males were not encountered with females (Shiao et al, 2013). However, the D. melanogaster males in courting behavior decrease expression of some immune response genes, probably correlated with the decreased immunity in mated males (Carney, 2007; McKean & Nunney, 2001).…”

Section: Resultsmentioning

confidence: 99%

Sex‐biased gene expression in antennae of Drosophila suzukii

Ahn

Corcoran

et al. 2020

Arch Insect Biochem Physiol

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Drosophila suzukii differs from other members of the genus Drosophila in its host preference and oviposition behavior. The flies are attracted to ripening fruits, and females have a serrated ovipositor enabling eggs to be laid inside the fruit. In addition to its huge economic impact, its unique chemoecological, morphological, and physiological characteristics have garnered considerable research interests. In this study, we analyzed D. suzukii antennal transcriptomes to identify sex‐biased genes by comparison of differential gene expressions between male antennae (MA) and female antennae (FA). Among 13,583 total genes of the fly genome, 11,787 genes were expressed in either MA or FA. There are only 132 genes (9 in MA, 7 in FA, and 116 in both, FPKM >1) were expressed in antennae exclusively, and 2,570 genes (9 in MA, 0 in FA, and 2,561 in both) were enriched in antennae containing 185 and 113 sex‐biased genes in MA and FA, respectively. Interestingly, many immune‐related genes were highly expressed in MA, whereas several chemosensory genes were at high rank in FA. We identified 27 sex‐biased chemosensory genes including odorant and gustatory receptors, odorant‐binding proteins, chemosensory proteins, ionotropic receptors, and cytochrome P450s, and validated the gene expressions using quantitative real‐time PCR. The highly expressed sex‐biased genes in antennae are likely involved in the fly specific mating, host‐finding behaviors, or sex‐specific functions. The molecular results demonstrated here will facilitate to find the unique chemoreception of D. suzukii, as well as on the development of new management strategies for this pest.

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“…; Shiao et al . ). These chemosensory genes include gustatory (GR), odorant (OR) and ionotropic (IR) receptors (Hallem et al .…”

Section: Introductionmentioning

confidence: 97%

Targeted re‐sequencing confirms the importance of chemosensory genes in aphid host race differentiation

et al. 2016

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Host-associated races of phytophagous insects provide a model for understanding how adaptation to a new environment can lead to reproductive isolation and speciation, ultimately enabling us to connect barriers to gene flow to adaptive causes of divergence. The pea aphid (Acyrthosiphon pisum) comprises host races specializing on legume species and provides a unique system for examining the early stages of diversification along a gradient of genetic and associated adaptive divergence. As host choice produces assortative mating, understanding the underlying mechanisms of choice will contribute directly to understanding of speciation. As host choice in the pea aphid is likely mediated by smell and taste, we use capture sequencing and SNP genotyping to test for the role of chemosensory genes in the divergence between eight host plant species across the continuum of differentiation and sampled at multiple locations across western Europe. We show high differentiation of chemosensory loci relative to control loci in a broad set of pea aphid races and localities, using a modelfree approach based on principal component analysis. Olfactory and gustatory receptors form the majority of highly differentiated genes and include loci that were already identified as outliers in a previous study focusing on the three most closely related host races. Consistent indications that chemosensory genes may be good candidates for local adaptation and barriers to gene flow in the pea aphid open the way to further investigations aiming to understand their impact on gene flow and to determine their precise functions in response to host plant metabolites.

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Transcriptional profiling of adult Drosophila antennae by high-throughput sequencing

Cited by 30 publications

References 32 publications

Population differences in olfaction accompany host shift in Drosophila mojavensis

Population differences in olfaction accompany host shift in Drosophila mojavensis

Sex‐biased gene expression in antennae of Drosophila suzukii

Targeted re‐sequencing confirms the importance of chemosensory genes in aphid host race differentiation

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