Transcriptional Profiling of Canker-Resistant Transgenic Sweet Orange (<i>Citrus sinensis</i>Osbeck) Constitutively Overexpressing a Spermidine Synthase Gene

Fu, Xing-Zheng; Liu, Jihong

doi:10.1155/2013/918136

Cited by 15 publications

(13 citation statements)

References 62 publications

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“…These complex results suggested that there are complex interactions among multiple genes and the exogenously expressed genes. The magnitude of the number differentially expressed genes found in this study is similar to previously reported ranges for transgenic plants harboring single transformation constructs [34,41,42]. …”

Section: Discussionsupporting

confidence: 90%

Transcriptome sequencing of transgenic poplar (Populus × euramericana 'Guariento') expressing multiple resistance genes

et al. 2014

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BackgroundTransgenic poplar (Populus × euramericana 'Guariento') plants harboring five exogenous, stress-related genes exhibit increased tolerance to multiple stresses including drought, salt, waterlogging, and insect feeding, but the complex mechanisms underlying stress tolerance in these plants have not been elucidated. Here, we analyzed the differences in the transcriptomes of the transgenic poplar line D5-20 and the non-transgenic line D5-0 using high-throughput transcriptome sequencing techniques and elucidated the functions of the differentially expressed genes using various functional annotation methods.ResultsWe generated 11.80 Gb of sequencing data containing 63, 430, 901 sequences, with an average length of 200 bp. The processed sequences were mapped to reference genome sequences of Populus trichocarpa. An average of 62.30% and 61.48% sequences could be aligned with the reference genomes for D5-20 and D5-0, respectively. We detected 11,352 (D5-20) and 11,372 expressed genes (D5-0), 7,624 (56.61%; D5-20) and 7,453 (65.54%; D5-0) of which could be functionally annotated. A total of 782 differentially expressed genes in D5-20 were identified compared with D5-0, including 628 up-regulated and 154 down-regulated genes. In addition, 196 genes with putative functions related to stress responses were also annotated. Gene Ontology (GO) analysis revealed that 346 differentially expressed genes are mainly involved in 67 biological functions, such as DNA binding and nucleus. KEGG annotation revealed that 36 genes (21 up-regulated and 15 down-regulated) were enriched in 51 biological pathways, 9 of which are linked to glucose metabolism. KOG functional classification revealed that 475 genes were enriched in 23 types of KOG functions.ConclusionThese results suggest that the transferred exogenous genes altered the expression of stress (biotic and abiotic) response genes, which were distributed in different metabolic pathways and were linked to some extent. Our results provide a theoretic basis for investigating the functional mechanisms of exogenous genes in transgenic plants.

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Section: Discussionsupporting

confidence: 90%

Transcriptome sequencing of transgenic poplar (Populus × euramericana 'Guariento') expressing multiple resistance genes

et al. 2014

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“…Consistent with these results, the increased production of hydrogen peroxide in sweet orange plants overexpressing an apple spermidine synthase gene (MdSPDS1) paralleled with the increased resistance to X. citri and augmented expression of defense-related genes (Fu et al 2011;Fu and Liu 2013). These results are also in agreement with the fact that canker symptoms attenuation in the transgenic plants overexpressing tobacco FLS2 or pepper Bs2 was concordant with an increase in ROS production (Hao et al 2016;Sendín et al 2017).…”

Section: Defense-related Genessupporting

confidence: 78%

Xanthomonas citri subsp. citri: host interaction and control strategies

Martins

Andrade

Benedetti

et al. 2020

Trop. plant pathol.

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Citrus is one of the most ancient fruit crops cultivated in the world. For many countries, citrus production is an important source of revenues. However, despite the richness of citrus production worldwide, fruit yields are constantly threatened by diseases that cause serious economic and social impacts to growers and consumers. One such example is citrus canker, a disease that affects all commercial citrus varieties and for which there is no cure. Currently, control measures for citrus canker are restricted to the application of copper-based compounds and the elimination of infected trees to prevent pathogen spreading in the field. However, new alternatives for the control of this disease are being developed, spanning through transgenic plants to innovative chemicals and biological control. New genome editing approaches and a repertoire of plant defense-related genes have been exploited to produce citrus lineages more tolerant to X. citri. In addition, a series of new molecules have been tested to not only inhibit X. citri growth but to also boost the plant immune system to suppress disease progression. In this review, we provide the state of art of all citrus canker control measures in use today, highlighting their utility and drawbacks and commenting on novel strategies to better control this important citrus disease.

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“…However, sweet orange plants modified in the same way were characterized by enhanced plant tolerance to Xanthomonas axonopodis pv. citri (Fu and Liu, 2013 ). Furthermore, overexpression of the SPMS gene in A. thaliana resulted in an increased spermidine content and enhanced resistance against Pseudomonas viridiflava (Gonzalez et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

Polyamine metabolism in flax in response to treatment with pathogenic and non–pathogenic Fusarium strains

et al. 2015

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Flax crop yield is limited by various environmental stress factors, but the largest crop losses worldwide are caused by Fusarium infection. Polyamines are one of the many plant metabolites possibly involved in the plant response to infection. However, in flax plants the polyamine composition, genes involved in polyamine synthesis, and in particular their regulation, were previously unknown. The aim of this study was to investigate the polyamine synthesis pathway in flax and its involvement in response to pathogen infection. It is well established that polyamines are essential for the growth and development of both plants and fungi, but their role in pathogen infection still remains unknown. In our study we correlated the expression of genes involved in polyamine metabolism with the polyamine levels in plant tissues and compared the results for flax seedlings treated with two pathogenic and one non-pathogenic strains of Fusarium. We observed an increase in the expression of genes participating in polyamine synthesis after fungal infection, and it was reflected in an increase of polyamine content in the plant tissues. The highest level of mRNA was characteristic for ornithine decarboxylase during infection with all tested, pathogenic and non-pathogenic, Fusarium strains and the arginine decarboxylase gene during infection with the pathogenic strain of Fusarium culmorum. The main polyamine identified in the flax seedlings was putrescine, and its level changed the most during infection. Moreover, the considerable increase in the contents of cell wall-bound polyamines compared to the levels of free and conjugated polyamines may indicate that their main role during pathogen infection lies in strengthening of the cell wall. In vitro experiments showed that the polyamines inhibit Fusarium growth, which suggests that they play an important role in plant defense mechanisms. Furthermore, changes in metabolism and content of polyamines indicate different defense mechanisms activated in flax in response to infection by pathogenic and non-pathogenic Fusarium strains.

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Transcriptional Profiling of Canker-Resistant Transgenic Sweet Orange (Citrus sinensisOsbeck) Constitutively Overexpressing a Spermidine Synthase Gene

Cited by 15 publications

References 62 publications

Transcriptome sequencing of transgenic poplar (Populus × euramericana 'Guariento') expressing multiple resistance genes

Transcriptome sequencing of transgenic poplar (Populus × euramericana 'Guariento') expressing multiple resistance genes

Xanthomonas citri subsp. citri: host interaction and control strategies

Polyamine metabolism in flax in response to treatment with pathogenic and non–pathogenic Fusarium strains

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