Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways

Transcriptional Regulation of the Respiratory Genes in the CyanobacteriumSynechocystissp. PCC 6803 during the Early Response to Glucose Feeding

“…In addition to carbohydrate catabolism-related genes, the genes coding for oxidative phosphorylation components, such as NDH-I, CtaI, and ATP synthase, are also regulated at the transcription level during the light-to-dark transition (Gill et al, 2002;Kucho et al, 2005). The coordinated regulation of carbohydrate catabolism and oxidative phosphorylation would ensure a close connection between the generation of cellular reducing power in the form of NAD(P)H during Glc catabolism and its subsequent utilization via the respiratory electron transport chain for the production of ATP: This has been reported previously for other prokaryotes, including Bacillus subtilis and Escherichia coli (Blencke et al, 2003;Vemuri et al, 2006). Despite the circumstantial evidence, a clear result demonstrating the relationship between respiratory activity and the coordinated regulation of respiratory genes at the transcriptional level in Synechocystis is still lacking.…”

Carbon Catabolite Control of the Metabolic Network inBacillus subtilis

“…Determination of the complete genome of B. subtilis 81) has made possible the detection of many genes that are probably subject to CcpA-mediated CCR and CCA by means of transcriptome and proteome analyses [91][92][93][94][95][96][97] as well as an electronical search for the cre sequence in the genome sequence. 25,93) Transcriptome and proteome analyses have revealed that out of the nearly 1,000 of the B. subtilis 4,107 protein genes whose expression in cells growing in a nutrient sporulation medium can be detected on DNA microarrays or 2D gels, roughly 10% are repressed or activated more than 3-fold upon the addition of glucose to the medium, 92) which implies that several hundred genes might be regulated by glucose.…”

“…Transcriptome and proteome analyses involving ccpA mutants [92][93][94][95]97) have revealed not only numerous genes whose expression is under CcpA-dependent catabolite control, but also not a few genes under CcpA-independent catabolite control. The individual induction systems of the catabolic genes are most likely involved in their CcpA-independent CCR, as described for iol, 92) except for those exerted by the other catabolite control proteins (CcpB, CcpC, and CcpN) as well as CggR which is involved in the regulation of central glycolytic genes.…”

Inactivation of the general transcription factor TnrA in Bacillus subtilis by proteolysis

“…Under nitrogen-limited growth, TnrA is a negative regulator of glnA and gltAB, which encode the ammonium assimilatory enzymes glutamine synthetase (GS) and glutamate synthase, respectively (Wray et al, 1996;Fisher & Debarbouille, 2002;Belitsky et al, 2000). Interestingly, the induction of the gltAB operon depends on the pleiotropic regulator of carbon metabolism CcpA, and requires sugars that can be catabolized via glycolysis (Faires et al, 1999;Blencke et al, 2003;Wacker et al, 2003).…”