Transcriptional profiling of the lectin ArathEULS3 from Arabidopsis thaliana toward abiotic stresses

Hove, Jonas Van; Stefanowicz, Karolina; Schutter, Kristof De; Eggermont, Lore; Lannoo, Nausicaä; Atalah, Bassam Al; Damme, Els J.M. Van

doi:10.1016/j.jplph.2014.08.009

Cited by 12 publications

(13 citation statements)

References 39 publications

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“…Transcript levels for ArathEULS3, the EUL homolog from Arabidopsis were also significantly upregulated after ABA treatment (Li et al 2014; Van Hove et al 2014), MeJA treatment (Van Hove et al 2014) and drought (Li et al 2014). In addition, Arabidopsis plants overexpressing ArathEULS3 revealed a better tolerance to drought stress (Li et al 2014) and Pseudomonas syringae infection (Van Hove et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, Arabidopsis plants overexpressing ArathEULS3 revealed a better tolerance to drought stress (Li et al 2014) and Pseudomonas syringae infection (Van Hove et al 2014). All these findings support the statement that EUL genes are actively involved in the signal cascades triggered by several abiotic stresses.…”

Section: Discussionmentioning

confidence: 99%

“…The genome of Arabidopsis thaliana harbours only one EUL gene, referred to as ArathEULS3 . The expression of this gene is upregulated after exposure to some plant hormones, drought and salt stress as well as Pseudomonas syringae infection (Li et al 2014; Van Hove et al 2014). Furthermore, it was shown that changes in expression levels of the lectin are accompanied with altered levels of stress resistance.…”

Section: Introductionmentioning

confidence: 99%

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Evolutionary relationships and expression analysis of EUL domain proteins in rice (Oryza sativa)

Schutter

Tsaneva

Kulkarni

et al. 2017

Rice

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Background: Lectins, defined as 'Proteins that can recognize and bind specific carbohydrate structures', are widespread among all kingdoms of life and play an important role in various biological processes in the cell. Most plant lectins are involved in stress signaling and/or defense. The family of Euonymus-related lectins (EULs) represents a group of stress-related lectins composed of one or two EUL domains. The latter protein domain is unique in that it is ubiquitous in land plants, suggesting an important role for these proteins. Results: Despite the availability of multiple completely sequenced rice genomes, little is known on the occurrence of lectins in rice. We identified 329 putative lectin genes in the genome of Oryza sativa subsp. japonica belonging to nine out of 12 plant lectin families. In this paper, an in-depth molecular characterization of the EUL family of rice was performed. In addition, analyses of the promoter sequences and investigation of the transcript levels for these EUL genes enabled retrieval of important information related to the function and stress responsiveness of these lectins. Finally, a comparative analysis between rice cultivars and several monocot and dicot species revealed a high degree of sequence conservation within the EUL domain as well as in the domain organization of these lectins.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evolutionary relationships and expression analysis of EUL domain proteins in rice (Oryza sativa)

Schutter

Tsaneva

Kulkarni

et al. 2017

Rice

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“…Arabidopsis overexpressing ArathEULS3 showed fewer disease symptoms after infection with P. syringae (Van Hove et al, 2015) and revealed increased tolerance to drought stress (Li et al, 2014). Additionally, the in-silico promoter analysis confirmed the presence of many cis-regulatory elements associated with drought/salt and ABA response in the promoter region upstream from the ArathEULS3 gene (Van Hove et al, 2014).…”

Section: Discussionmentioning

confidence: 60%

“…ArathEULS3 is a stress-responsive protein that locates to the nucleo-cytoplasmic compartment of the plant cell (Van Hove et al, 2011). The expression of ArathEULS3 is enhanced after hormone treatments (ABA, MeJA, Ethephon) as well as after abiotic (drought, osmotic stress), or biotic (Pseudomonas syringae) stresses (Van Hove et al, 2014). In 2014, Li et al suggested that ArathEULS3 is involved in ABA signaling through interaction with the ABA receptor PYL9.…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis Lectin EULS3 Is Involved in ABA Signaling in Roots

et al. 2020

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The Arabidopsis thaliana lectin ArathEULS3 is upregulated in particular stress conditions and upon abscisic acid (ABA) treatment. ABA is a plant hormone important for plant growth and stress responses. During stress ABA is perceived by PYR/PYL/RCAR receptors, inhibiting protein phosphatases PP2Cs thereby enabling SNRK2s kinases to start downstream phosphorylation cascades and signaling. PYL9, one of the ABA receptors was identified as an interacting partner for ArathEULS3. Promoter::GUS activity studies revealed the expression of ArathEULS3 in the central root cylinder and the cells flanking young lateral root primordia, and showed enhanced expression in root tips after ABA treatment. Transcript levels for ArathEULS3 increased after exposure to ABA and osmotic treatments. ArathEULS3 CRISPR KO mutants served as a tool to expand the knowledge on the role of ArathEULS3 in plant development. KO lines revealed a longer root system compared to WT plants, and showed reduced sensitivity to ABA, salt, and osmotic conditions. Additionally it was noted that the KO mutants had more emerged lateral roots when grown in high osmotic conditions. Together these data suggest that ArathEULS3 may be an important player in ABA responses in roots.

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