Transcriptional Profiling Reveals the Importance of RcrR in the Regulation of Multiple Sugar Transportation and Biofilm Formation in Streptococcus mutans

Gong, Tao; He, Xiao; Chen, Jiamin; Tang, Boyu; Zheng, Ting; Jing, Meiling; Lin, Yi‐Dan; Pan, Yiqi; Ma, Qizhao; Li, Yuqing; Zhou, Xuedong

doi:10.1128/msystems.00788-21

Cited by 16 publications

(10 citation statements)

References 35 publications

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“…3D reconstruction of the z-stacks was performed using Imaris software (Oxford Instruments), and percentage viability and biomass were computed as previously described. 32 , 33 Subsequently, for the bacterial challenge in the biofilm state, the coverslips with biofilms were placed on top of the CTEs with the bacterial front facing the tissue and incubated for 24 h. After bacterial challenge in planktonic and biofilm state, the culture supernatant was collected, centrifuged to remove cellular debris, and stored at −80°C for downstream cytokine analysis.…”

Section: Methodsmentioning

confidence: 99%

Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization

et al. 2022

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Gingival and periodontal ligament fibroblasts are functionally distinct cell types within the dento-gingival unit that participate in host immune response. Their microenvironment influences the behavior and immune response to microbial challenge. We developed three-dimensional gingival and periodontal connective tissue equivalents (CTEs) using human fibrin-based matrix. The CTEs were characterized, and the heterogeneity in their innate immune response was investigated. The CTEs demonstrated no to minimal response to planktonic Streptococcus mitis and Streptococcus oralis, while their biofilms elicited a moderate increase in IL-6 and IL-8 production. In contrast, Fusobacterium nucleatum provoked a substantial increase in IL-6 and IL-8 production. Interestingly, the gingival CTEs secreted significantly higher IL-6, while periodontal counterparts produced higher IL-8. In conclusion, the gingival and periodontal CTEs exhibited differential responses to various bacterial challenges. This gives insights into the contribution of tissue topography and fibroblast heterogeneity in rendering protective and specific immune responses toward early biofilm colonizers.

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Section: Methodsmentioning

confidence: 99%

Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization

et al. 2022

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“…We aimed to determine whether L. paracasei ET-22 could affect the adhesion of S. mutans. The initial adhesion of S. mutans is achieved when it combines with the membrane receptors on the tooth surface via sucrose-independent weak interactions, which are mediated by long-range forces, i.e., van der Waals forces and Coulomb interactions, and short-range forces, including electrostatic interactions and hydrophobic interactions [ 24 , 25 , 26 ]. SPAP, a non-sucrose-dependent adhesion protein, is initially and primarily involved in promoting the colonization of S. mutans on tooth surfaces by specific interactions with salivary agglutinins [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Postbiotics Derived from L. paracasei ET-22 Inhibit the Formation of S. mutans Biofilms and Bioactive Substances: An Analysis

Zhao

Sun

et al. 2023

Molecules

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Globally, dental caries is one of the most common non-communicable diseases for patients of all ages; Streptococcus mutans (S. mutans) is its principal pathogen. Lactobacillus paracasei (L. paracasei) shows excellent anti-pathogens and immune-regulation functions in the host. The aim of this study is to evaluate the effects of L. paracasei ET-22 on the formation of S. mutans biofilms. The living bacteria, heat-killed bacteria, and secretions of L. paracasei ET-22 were prepared using the same number of bacteria. In vitro, they were added into artificial-saliva medium, and used to coculture with the S. mutans. Results showed that the living bacteria and secretions of L. paracasei ET-22 inhibited biofilm-growth, the synthesis of water-soluble polysaccharide and water-insoluble polysaccharide, and virulence-gene-expression levels related to the formation of S. mutans biofilms. Surprisingly, the heat-killed L. paracasei ET-22, which is a postbiotic, also showed a similar regulation function. Non-targeted metabonomics technology was used to identify multiple potential active-substances in the postbiotics of L. paracasei ET-22 that inhibit the formation of S. mutans biofilms, including phenyllactic acid, zidovudine monophosphate, and citrulline. In conclusion, live bacteria and its postbiotics of L. paracasei ET-22 all have inhibitory effects on the formation of S. mutans biofilm. The postbiotics of L. paracasei ET-22 may be a promising biological anticariogenic-agent.

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“…The markerless in-frame deletion mutant of the S. mutans GNAT family member ActA was constructed using a previously described two-step transformation method ( 59 , 60 ). Table S5 lists all of the primers designed in this study.…”

Section: Methodsmentioning

confidence: 99%

Acetylation of Lactate Dehydrogenase Negatively Regulates the Acidogenicity of Streptococcus mutans

Pan

Chen

et al. 2022

mBio

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Transcriptional Profiling Reveals the Importance of RcrR in the Regulation of Multiple Sugar Transportation and Biofilm Formation in Streptococcus mutans

Cited by 16 publications

References 35 publications

Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization

Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization

Postbiotics Derived from L. paracasei ET-22 Inhibit the Formation of S. mutans Biofilms and Bioactive Substances: An Analysis

Acetylation of Lactate Dehydrogenase Negatively Regulates the Acidogenicity of Streptococcus mutans

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