Transcriptional regulation during Drosophila spermatogenesis

Lim, Cindy; Tarayrah, Lama; Chen, Xin

doi:10.4161/spmg.21775

Cited by 38 publications

(26 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Primary spermatocytes produce transcripts that are used at three different time points in spermatogenesis (reviewed in refs. 32 and 33). The first group of transcripts are produced to allow for the expansive growth that takes place during the immature primary spermatocyte stage.…”

Section: Drosophila Spermatogenesis: Cytological and Molecular Overviewmentioning

confidence: 98%

Protamines and spermatogenesis inDrosophilaandHomo sapiens

2013

View full text Add to dashboard Cite

The production of mature and motile sperm is a detailed process that utilizes many molecular players to ensure the faithful execution of spermatogenesis. In most species that have been examined, spermatogenesis begins with a single cell that undergoes dramatic transformation, culminating with the hypercompaction of DNA into the sperm head by replacing histones with protamines. Precise execution of the stages of spermatogenesis results in the production of motile sperm. While comparative analyses have been used to identify similarities and differences in spermatogenesis between species, the focus has primarily been on vertebrate spermatogenesis, particularly mammals. To understand the evolutionary basis of spermatogenetic variation, however, a more comprehensive comparison is needed. In this review, we examine spermatogenesis and the final packaging of DNA into the sperm head in the insect Drosophila melanogaster and compare it to spermatogenesis in Homo sapiens.

show abstract

Section: Drosophila Spermatogenesis: Cytological and Molecular Overviewmentioning

confidence: 98%

Protamines and spermatogenesis inDrosophilaandHomo sapiens

2013

View full text Add to dashboard Cite

show abstract

“…All the 64 spermatids in a cyst simultaneously differentiate, synchronously resulting in a bundle of parallel elongated spermatids. Finally, the Drosophila spermatid undergoes dramatic morphological changes as it differentiates from a small round cell into a highly polarized motile sperm, with a concentrated needle‐shaped head and a long tail, a process that involves many genes (Lim et al ., ). Amongst the generally expressed genes, there can also be a switch to expression of a testis‐specific isoform, using either a novel transcription start site or testis‐specific splicing pattern (Gan et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Ocnus is essential for male germ cell development in Drosophila melanogaster

Zheng

Hou

et al. 2018

Insect Molecular Biology

View full text Add to dashboard Cite

The ocnus (ocn) gene encodes a protein abundant in the testes, implying its role in testis development. When Drosophila melanogaster is infected with the endosymbiont wMel Wolbachia, which affects the spermatogenesis of its hosts, ocn is downregulated in the third-instar larval testes, suggesting a role of ocn in spermatogenesis. In this study, we knocked down ocn in the testes and found that the hatch rates of embryos derived from ocn-knockdown males were significantly decreased, and 84.38% of the testes were much smaller in comparison to controls. Analysis of the smaller testes showed no germ cells but they had an extended hub. Using RNA-sequencing (RNA-Seq), we identified 69 genes with at least a twofold change (q-value < 5%) in their expression after ocn knockdown; of these, eight testes-specific and three reproduction-related genes were verified to be significantly downregulated using quantitative reverse transcription-PCR. Three genes (orientation disruptor, p24-2 and CG13541) were also significantly downregulated in the presence of Wolbachia. Furthermore, 98 genes were not expressed when ocn was knocked down in testes. These results suggest that ocn plays a crucial role in male germ cell development in Drosophila, possibly by regulating the expression of multiple spermatogenesis-related genes. Our data provide important information to help understand the molecular regulatory mechanisms underlying spermatogenesis.

show abstract

“…1B, S1) [25][26][27][28] . To disrupt male fertility, we targeted genes active during spermatogenesis, such as βTubulin 85D ( βTub, sgRNA βTub ) 29 , fuzzy onions (fzo, sgRNA Fzo ) 30 , protamine A (ProtA, sgRNA ProtA ) 31 , or spermatocyte arrest (sa, sgRNA Sa ) 32 (Fig. S1).…”

Section: Binary Crispr Induced Female Masculinization/lethality or Mmentioning

confidence: 99%

Transforming Insect Population Control with Precision Guided Sterile Males

Kandul

Liu

Sánchez

et al. 2018

Preprint

View full text Add to dashboard Cite

The sterile insect technique (SIT) is an environmentally safe and proven technology to suppress wild populations. To further advance its utility, a novel CRISPR-based technology termed "precision guided SIT" (pgSIT) is described. PgSIT mechanistically relies on a dominant genetic technology that enables simultaneous sexing and sterilization, facilitating the release of eggs into the environment ensuring only sterile adult males emerge. Importantly, for field applications, the release of eggs will eliminate burdens of manually sexing and sterilizing males, thereby reducing overall effort and increasing scalability. To demonstrate efficacy, we systematically engineer multiple pgSIT systems in Drosophila which consistently give rise to 100% sterile males. Importantly, we demonstrate that pgSIT-generated males are fit and competitive. Using mathematical models, we predict pgSIT will induce substantially greater population suppression than can be achieved by currently-available self-limiting suppression technologies. Taken together, pgSIT offers to transform our ability to control insect agricultural pests and disease vectors.

show abstract

Transcriptional regulation during Drosophila spermatogenesis

Cited by 38 publications

References 95 publications

Protamines and spermatogenesis inDrosophilaandHomo sapiens

Protamines and spermatogenesis inDrosophilaandHomo sapiens

Ocnus is essential for male germ cell development in Drosophila melanogaster

Transforming Insect Population Control with Precision Guided Sterile Males

Contact Info

Product

Resources

About