Transcriptional regulation of 9-cis-epoxycarotenoid dioxygenase (NCED) gene by putrescine accumulation positively modulates ABA synthesis and drought tolerance in Lotus tenuis plants

Espasandin, Fabiana Daniela; Maiale, Santiago Javier; Calzadilla, Pablo Ignacio; Ruíz, Oscar Adolfo; Sansberro, Pedro Alfonso

doi:10.1016/j.plaphy.2013.12.018

Cited by 77 publications

(52 citation statements)

References 32 publications

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“…Recently the role of endogenously produced Put affecting the expression of drought responsive gene 9-cis-epoxycarotenoid dioxygenase (NCED) - can enzyme that controls ABA biosynthesis under stress, was studied in lotus ( Lotus tenuis ), using a heterologous oat ( Avena sativa ) ADC gene under the control of a drought/ABA inducible promoter RD29A (Espasandin et al, 2014). Drought increased the expression of oat ADC by ~100-fold, total ADC activity by ~2-fold and Put by ~3.6-fold, with only minor changes in Spd and Spm.…”

Section: Polyamines and Abiotic Stress In Plantsmentioning

confidence: 99%

Polyamines and abiotic stress in plants: a complex relationship1

2014

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The physiological relationship between abiotic stress in plants and polyamines was reported more than 40 years ago. Ever since there has been a debate as to whether increased polyamines protect plants against abiotic stress (e.g., due to their ability to deal with oxidative radicals) or cause damage to them (perhaps due to hydrogen peroxide produced by their catabolism). The observation that cellular polyamines are typically elevated in plants under both short-term as well as long-term abiotic stress conditions is consistent with the possibility of their dual effects, i.e., being protectors from as well as perpetrators of stress damage to the cells. The observed increase in tolerance of plants to abiotic stress when their cellular contents are elevated by either exogenous treatment with polyamines or through genetic engineering with genes encoding polyamine biosynthetic enzymes is indicative of a protective role for them. However, through their catabolic production of hydrogen peroxide and acrolein, both strong oxidizers, they can potentially be the cause of cellular harm during stress. In fact, somewhat enigmatic but strong positive relationship between abiotic stress and foliar polyamines has been proposed as a potential biochemical marker of persistent environmental stress in forest trees in which phenotypic symptoms of stress are not yet visible. Such markers may help forewarn forest managers to undertake amelioration strategies before the appearance of visual symptoms of stress and damage at which stage it is often too late for implementing strategies for stress remediation and reversal of damage. This review provides a comprehensive and critical evaluation of the published literature on interactions between abiotic stress and polyamines in plants, and examines the experimental strategies used to understand the functional significance of this relationship with the aim of improving plant productivity, especially under conditions of abiotic stress.

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Section: Polyamines and Abiotic Stress In Plantsmentioning

confidence: 99%

Polyamines and abiotic stress in plants: a complex relationship1

2014

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“…Abscisic acid also influences the catabolism of polyamines as it induces the expression of PAO [59], while at the same time, polyamines influence abscisic acid synthesis [30]. The transcriptional regulation of the 9-cis-epoxycarotenoid dioxygenase gene (NCED), which encodes the key enzyme involved in abscisic acid biosynthesis via putrescine accumulation, has been reported in ADC overexpressing transgenic plants under stress conditions [60][61]. Conversely, the suppression of arginine decarboxylase (in both adc1 and adc2 plants) resulted in the reduced expression of NCED3 and the down-regulation of abscisic acid-regulated genes.…”

Section: Connection With Hormones and Other Small Hormone-like Protecmentioning

confidence: 99%

Speculation: Polyamines are important in abiotic stress signaling

2015

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The main role of polyamines was originally assumed to be as direct protective compounds important under stress conditions. Although in some cases a correlation was found between the endogenous polyamine content and stress tolerance, this relationship cannot be generalized. Polyamines should no longer be considered simply as protective molecules, but rather as compounds that are involved in a complex signaling system and have a key role in the regulation of stress tolerance. The major links in polyamine signaling may be H 2 O 2 and NO, which are not only produced in the course of the polyamine metabolism, but also transmit signals that influence gene expression via an increase in the cytoplasmic Ca 2+ level.Polyamines can also influence Ca 2+ influx independently of the H 2 O 2 -and/or NO-mediated pathways. Furthermore, these pathways may converge. In addition, several protein kinases have been shown to be influenced at the transcriptional or post-translational level by polyamines. Individual polyamines can be converted into each other in the polyamine cycle.In addition, their metabolism is linked with other hormones or signaling molecules. However, as individual polyamines trigger different transcriptional responses, other mechanisms and the existence of polyamine-responsive elements and the corresponding transacting protein factors are also involved in polyamine-related signaling pathways. Highlights:-Polyamines are interconvertible in the polyamine cycle -The statement "the higher the polyamine level the better" cannot be generalized -In stress responses the ratio of signaling to direct protection is more important -Polyamines are also involved in hormonal cross-talk -H 2 O 2 and NO are the major but not the only links in polyamine stress signaling

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“…The constitutively overexpression of oat arginine decarboxylase 2 gene in M. truncatula (Duque et al, 2016), and driven by the stress-inducible RD29A promotor in L. tenuis (Espasandin et al, 2014), improved the physiological responses to water deficit of drought-stress plants (Espasandin et al, 2014), also demonstrating that Put controls the abscisic acid (ABA) content in response to drought, by modulating the expression of 9-cis-epoxycarotenoid dioxygenase (NCED, which catalyzes a regulatory step in ABA biosynthesis). Last result is part of a bulk of evidence supporting the theory that Put and ABA are assimilated in a positive feedback loop, in which ABA and Put communally promote each other's biosynthesis in response to abiotic stress (see ).…”

Section: Drought Stressmentioning

confidence: 99%

“…As the intensity of stress increases, PAs promotes osmoregulation and preserves the leaf relative water content. This effect is due to accumulation of either, proline (Cicer arietinum, G. max, L. tenuis) or γ-aminobutyric acid (GABA) (T. repens) at the cellular level (Espasandin et al, 2014;Nayyar et al, 2005;Yong et al, 2017). Likewise, the exogenous application of Spd plays a vital role in accelerating starch metabolism by increasing α-and β-amylase activities during the germination process of T. repens seeds, under low soil water content (Li et al, 2014a), and leads to the accumulation sucrose, fructose, sorbitol, and dehydrins in leaves (Li et al, 2015) during water deprivation.…”

Section: Drought Stressmentioning

confidence: 99%

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Polyamines and legumes: Joint stories of stress, nitrogen fixation and environment

Menéndez

Calzadilla

Sansberro

et al. 2019

Preprint

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Polyamines (PAs) are natural aliphatic amines involved in many physiological processes in almost all living organisms, including responses to abiotic stresses and microbial interactions. This review presents the profuse evidence that relates changes in polyamines levels during responses to biotic and abiotic stresses in model and cultivable species within Leguminosae, and examines their potential roles in the functioning of symbiotic interactions with nitrogen fixing bacteria and arbuscular mycorrhizae. The family Leguminosae constitutes an economically and ecologically key botanical group for humans, being also regarded as the most important protein source for livestock. The ability of legumes to establish symbiotic interactions with nitrogen fixing bacteria and arbuscular mycorrhizae fungi, gives to some legume species that are able to exploit their molecular machinery “pioneer” attributes, with better competition in nutrient-poor soils and higher adaptation to restricted environments. However, many legume crops may be affected by climate change-derived environmental stresses, whereby maintaining their yields safe from adverse environmental conditions is probably one of the biggest challenge facing modern agriculture. Therefore, the obtaining of vigorous genotypes with higher tolerance to abiotic and biotic stressors has turned an increasingly important biotechnological target. At this scenario, PAs can play an important role, and genetic manipulation of crop plants with genes encoding polyamine PA biosynthetic pathway enzymes is envisioned as a strategy to achieve plants with improved stress tolerance and symbiotic performance. As linking plant physiological behavior with "big data" available in "omics" is an essential step to improve our understanding of legumes responses to global change, we also examined integrative MultiOmics approaches available to decrypt the interface legumes-PAs-abiotic and biotic stress or interactions. These approaches are expected to accelerate the identification of stress tolerant phenotypes and the design of new biotechnological strategies to increase their yield and adaptation to marginal environments, making a better use of available plant genetic resources.

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Transcriptional regulation of 9-cis-epoxycarotenoid dioxygenase (NCED) gene by putrescine accumulation positively modulates ABA synthesis and drought tolerance in Lotus tenuis plants

Cited by 77 publications

References 32 publications

Polyamines and abiotic stress in plants: a complex relationship1

Polyamines and abiotic stress in plants: a complex relationship1

Speculation: Polyamines are important in abiotic stress signaling

Polyamines and legumes: Joint stories of stress, nitrogen fixation and environment

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