Transcriptional regulation of Arabidopsis thaliana WRKY genes under interaction with beneficial fungus Trichoderma atroviride

Sáenz‐Mata, Jorge; Salazar-Badillo, Fatima Berenice; Jiménez-Bremont, Juan Francisco

doi:10.1007/s11738-013-1483-7

Cited by 24 publications

(15 citation statements)

References 53 publications

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“…In this context, Tucci et al ( 2010 ) found some differences in plant growth in several tomato lines induced by T. harzianum T22 and T. atroviride P1, demonstrating that T22 is more effective than P1, and that this feature depends on the tomato genotype. Increasing lines of evidence have shown that the direct effect exerted by Trichoderma on plants is through the production of phytohormones, phytohormone-like molecules, volatile organic compounds, secondary metabolites, or by altering the plant phytohormone homeostasis (Gravel et al, 2007 ; Vinale et al, 2008 ; Contreras-Cornejo et al, 2009 ; Viterbo and Horwitz, 2010 ; Salas-Marina et al, 2011 ; Olmedo-Monfil and Casas-Flores, 2014 ; Sáenz-Mata et al, 2014 ). Trichoderma spp.…”

Section: Discussionmentioning

confidence: 99%

The Epl1 and Sm1 proteins from Trichoderma atroviride and Trichoderma virens differentially modulate systemic disease resistance against different life style pathogens in Solanum lycopersicum

et al. 2015

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Fungi belonging to the genus Trichoderma, commonly found in soil or colonizing plant roots, exert beneficial effects on plants, including the promotion of growth and the induction of resistance to disease. T. virens and T. atroviride secrete the proteins Sm1 and Epl1, respectively, which elicit local and systemic disease resistance in plants. In this work, we show that these fungi promote growth in tomato (Solanum lycopersicum) plants. T. virens was more effective than T. atroviride in promoting biomass gain, and both fungi were capable of inducing systemic protection in tomato against Alternaria solani, Botrytis cinerea, and Pseudomonas syringae pv. tomato (Pst DC3000). Deletion (KO) of epl1 in T. atroviride resulted in diminished systemic protection against A. solani and B. cinerea, whereas the T. virens sm1 KO strain was less effective in protecting tomato against Pst DC3000 and B. cinerea. Importantly, overexpression (OE) of epl1 and sm1 led to an increase in disease resistance against all tested pathogens. Although the Trichoderma WT strains induced both systemic acquired resistance (SAR)- and induced systemic resistance (ISR)-related genes in tomato, inoculation of plants with OE and KO strains revealed that Epl1 and Sm1 play a minor role in the induction of these genes. However, we found that Epl1 and Sm1 induce the expression of a peroxidase and an α-dioxygenase encoding genes, respectively, which could be important for tomato protection by Trichoderma spp. Altogether, these observations indicate that colonization by beneficial and/or infection by pathogenic microorganisms dictates many of the outcomes in plants, which are more complex than previously thought.

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Section: Discussionmentioning

confidence: 99%

The Epl1 and Sm1 proteins from Trichoderma atroviride and Trichoderma virens differentially modulate systemic disease resistance against different life style pathogens in Solanum lycopersicum

et al. 2015

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“…The Arabidopsis transcription factors MYB72 and WRKY8 are also needed for the onset of ISR mediated by rhizosphere bacteria and fungi, respectively (Van der Ent et al, 2008b;Saenz-Mata et al, 2014). Compared to the wild type, both mutants showed distinct exudation patterns, including lower amounts of asparagine, ornithine and tryptophan, as well as bacterial and archaeal community composition, as illustrated by an increased abundance of Streptomyces, Bacillus and Lysinibacillus taxa in the med25 rhizosphere and an Enterobacteriaceae population in myc2.…”

Section: Mpmimentioning

confidence: 99%

“…The Arabidopsis transcription factors MYB72 and WRKY8 are also needed for the onset of ISR mediated by rhizosphere bacteria and fungi, respectively (Van der Ent et al, 2008b;Saenz-Mata et al, 2014). Exogenous treatment with JA has recently been shown to alter root exudate profiles and the composition of root-associated bacterial communities.…”

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confidence: 99%

Linking Jasmonic Acid Signaling, Root Exudates, and Rhizosphere Microbiomes

Carvalhais

Dennis

Badri

et al. 2015

MPMI

252

187

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Jasmonic acid (JA) is an essential hormone in plant development and defense responses in Arabidopsis thaliana. Exogenous treatment with JA has recently been shown to alter root exudate profiles and the composition of root-associated bacterial communities. However, it is currently unknown whether disruptions of the JA in the rhizosphere affect root exudation profiles and the relative abundance of bacteria and archaea in the rhizosphere. In the present study, two Arabidopsis mutants that are disrupted in different branches of the jasmonate pathway, namely myc2 and med25, were cultivated in nutrient solution and soil to profile root exudates and bacterial and archaeal communities, respectively. Compared with the wild type, both mutants showed distinct exudation patterns, including lower amounts of asparagine, ornithine, and tryptophan, as well as distinct bacterial and archaeal community composition, as illustrated by an increased abundance of Streptomyces, Bacillus, and Lysinibacillus taxa in the med25 rhizosphere and an Enterobacteriaceae population in myc2. Alternatively, the Clostridiales population was less abundant in the rhizosphere of both mutants. Similarities between plant genotypes were highly correlated, as determined by operational taxonomic units in the rhizosphere and metabolites in root exudates. This strongly suggests that root exudates play a major role in modulating changes in microbial community composition upon plant defense responses.

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“…The WRKY proteins, which contain one or two conserved WRKY domains consisting of 60 amino acid region with a conserved WRKYGQK motif in its N-terminus followed by a C 2 H 2 or C 2 HC zinc-finger motif, represent a large family of plant-specific TFs (Rushton et al 2010). There are over 70 WRKY genes in Arabidopsis (Eulgem et al 2000;Dong et al 2003;Sáenz-Mata et al 2014). Studies on WRKY genes predominantly point to an involvement in salicylic acid (SA) signaling and disease responses (Eulgem et al 2000;Asai et al 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Studies on WRKY genes predominantly point to an involvement in salicylic acid (SA) signaling and disease responses (Eulgem et al 2000;Asai et al 2002). In addition, WRKY genes are involved in plant responses to drought, heat, cold, salinity (Zhou et al 2008;Liu et al 2014;Sáenz-Mata et al 2014;Cheng et al 2017;Ye et al 2017;Han et al 2018), freezing (Huang & Duman 2002), wounding (Hara et al, 2000), and oxidative stress (Rizhsky et al 2004;Cui et al 2018). But these studies mostly focused on model plants or crops, and the roles of the WRKY genes in stress responses of Malus plant were less well known.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and functional analysis of MbWRKY3 involved in improved drought tolerance in transformed tobacco

Han

Zhang

Ding

et al. 2018

Journal of Plant Interactions

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Plant-specific WRKY transcription factors were involved in stress responses and ABA signaling. In the present study, a WRKY gene is isolated from Malus baccata (L.) Borkh and designated as MbWRKY3. Subcellular localization revealed that MbWRKY3 was localized onto nucleus. The MbWRKY3 expression levels were up-regulated by salinity, drought, and ABA treatments in M. baccata. When MbWRKY3 was introduced into tobaccos, it improved drought stress tolerance in transgenic plants. Compared to WT, the transgenic tobaccos had the higher levels of relative water content, and proline and chlorophyll contents, decreased levels of electrolyte leakage, MDA, and H 2 O 2 , increased activities of the reactive oxygen species-related enzymes (SOD, CAT, and POD), and greater upregulations of the corresponding genes (NtSOD, NtCAT, and NtPOD), especially when dealt with drought stress. These results suggest that MbWRKY3 gene plays a positive regulatory role in drought stress response.

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Transcriptional regulation of Arabidopsis thaliana WRKY genes under interaction with beneficial fungus Trichoderma atroviride

Cited by 24 publications

References 53 publications

The Epl1 and Sm1 proteins from Trichoderma atroviride and Trichoderma virens differentially modulate systemic disease resistance against different life style pathogens in Solanum lycopersicum

The Epl1 and Sm1 proteins from Trichoderma atroviride and Trichoderma virens differentially modulate systemic disease resistance against different life style pathogens in Solanum lycopersicum

Linking Jasmonic Acid Signaling, Root Exudates, and Rhizosphere Microbiomes

Molecular cloning and functional analysis of MbWRKY3 involved in improved drought tolerance in transformed tobacco

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