Transcriptional regulation of chlorogenic acid biosynthesis in carrot root slices exposed to UV-B light

Abstract

Orange carrots are well known for their nutritional value as producers of ß-carotene, a vitamin A precursor. Lesser known, is their ability to accumulate antioxidants such as chlorogenic acid. Chlorogenic acid is produced through the same biosynthetic pathway that produces lignins, anthocyanins, flavonols and isoflavonoids, the phenylpropanoid pathway. This pathway can be induced by UV-B light and wounding to produce large amounts of chlorogenic acid. We identified a number of the carrot structural genes in this pathway and their proposed regulators in carrot root slices and analyzed their expression in response to UV-B light exposure using real-time PCR. Our results indicate that not only does the expression of the structural genes correlate with their proposed regulators, including DcHY5 and others, but also correlate with chlorogenic acid production. Our results indicate that the HY5-UVR8 signaling network may exist and function in carrot roots.

High-quality de novo assembly of the Eucommia ulmoides haploid genome provides new insights into evolution and rubber biosynthesis

“…These genes may be key genes whose proteins catalyze CGA biosynthesis in E. ulmoides . In addition, some transcription factors (TFs) have also been reported to regulate CGA biosynthesis, including the MYB , ZIP , and WRKY families 61 – 64 . We identified 1,406 TF-encoding genes in E. ulmoides , representing 5.41% of the total genes.…”

Integrated transcriptome, small RNA and degradome sequencing approaches proffer insights into chlorogenic acid (CGA) biosynthesis in leafy sweet potato

“…2a-c). Although a few genes involved in CGA biosynthesis had been reported in other plant species [14,[22][23][24][25][26][27][28][29][30], the molecular mechanisms underlying in leafy sweet potato remains largely unknown. In the present study, a total of 29834 genes were identi ed based on the reference guided transcriptome analysis of two genotypes at two stages.…”

Integrated transcriptome, small RNA and degradome sequencing approaches proffer insights into chlorogenic acid (CGA) biosynthesis in leafy sweet potato

“…2a-c). Although a few genes involved in CGA biosynthesis had been reported in other plant species [14,[22][23][24][25][26][27][28][29][30], the molecular mechanisms underlying in leafy sweet potato remains largely unknown. In the present study, a total of 29834 genes were identified based on the reference guided transcriptome analysis of two genotypes at two stages.…”

“…Some TFs were also reported to regulate the biosynthesis of CGA. MYB1 was an important transcriptional activator of C4H (PAL1),, while MYB 3 and 5 were found to act on the promotor of PAL3 in carrots [28]. ZIP8 could be specifically bound to the G-box element of PAL2 5'-UTR in Lonicera japonica, its overexpressing leading to a decrease in CGA content [26].…”