Transcriptional regulation of cytokines in the intestine of Atlantic cod fed yeast derived mannan oligosaccharide or β-Glucan and challenged with Vibrio anguillarum

Lokesh, Jep; Fernandes, Jorge M.O.; Korsnes, Kjetil; Bergh, Øivind; Brinchmann, Monica F.; Kiron, Viswanath

doi:10.1016/j.fsi.2012.06.017

Cited by 119 publications

(64 citation statements)

References 39 publications

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“…However, food safety is of upmost importance, therefore diligent research and stringent regulations must be required before commercialization of GM products. The balance between nutritious, safe food production and maintaining environmental sustainability will rely on our ability to use new technologies to improve farming practices, and our ability to use as few resources as possible to provide for our society [80][81][82][83][84][85][86][87][88][89][90][91][92][93][94][95].…”

Section: Resultsmentioning

confidence: 99%

Fish Nutrition and Current Issues in Aquaculture: The Balance in Providing Safe and Nutritious Seafood, in an Environmentally Sustainable Manner

Hixson¹

2014

J Aquac Res Development

113

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Global aquaculture production has increased in recent years and it is predicted that aquaculture will provide the most reliable supply of seafood in the future. However, there are many controversial issues in aquaculture regarding food safety, nutrition, and sustainability; many of which are directly related to the nutrition and feeds for farmed fish. These nutrition-related issues must be considered in order to achieve balance in safe and nutritious food production and sustainability in aquaculture. This review highlights recent studies and discusses new and innovative aspects in fish nutrition. Some issues in the area of fish nutrition require consideration and improvement, such as: feed and nutrient efficiency, overfeeding and waste, fish meal and fish oil replacements, fish health, biotechnology, and human health concerns. The findings reviewed in this manuscript demonstrate promise toward improvement of the aquaculture industry through nutrition. This review is an update in fish nutrition research, and provides insight on the progression and evolution of this field in order to meet the needs of the industry with the purpose to achieve a balance in seafood production and environmental sustainability. The outcome of this review encourages the use of biotechnology as a tool to meet seafood production and environmental sustainability, in order to ensure global food security in the future and to improve our resource use.

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Section: Resultsmentioning

confidence: 99%

Fish Nutrition and Current Issues in Aquaculture: The Balance in Providing Safe and Nutritious Seafood, in an Environmentally Sustainable Manner

Hixson¹

2014

J Aquac Res Development

113

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“…RNA was extracted from 50 mg of skin from five specimens of gilthead seabream for each treatment group and control group using QIAzol lysis reagent method (Qiagen) as described elsewhere [30]. Quality of total RNA was checked on 1% agarose gel, followed by the quantification using the Qubit ® RNA were converted into relative quantities.…”

Section: Gene Expression Analysismentioning

confidence: 99%

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Cordero¹,

Morcillo

Cuesta

et al. 2016

Journal of Proteomics

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Differential proteome profile of skin mucus of gilthead seabream (sparus aurata) after probiotic intake and/or overcrowding stress, Journal of Proteomics (2015Proteomics ( ), doi: 10.1016Proteomics ( /j.jprot.2015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E DM A N U S C R I P T ACCEPTED MANUSCRIPT A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT2 AbstractGilthead seabream (Sparus aurata L.) is the major cultured fish species in the Mediterranean area. High density stocking causes stress and increases the impact of diseases leading to economic losses. Probiotics could represent a solution to prevent diseases through several mechanisms such as improving the immune status and/or mucosal microbiota or competing with pathogens. The probiotic Shewanella putrefaciens, also known as Pdp11, was firstly isolated from the skin of healthy gilthead seabream. Our study focuses on the skin mucus proteome after dietary probiotic Pdp11 intake in fish maintained under normal or overcrowding conditions. 2-DE of skin mucus followed by LC-MS/MS analysis was done for each experimental group and differentially expressed proteins were identified. The results showed differentially expressed proteins especially involved in immune processes, such as lysozyme, complement C3, natural killer cell enhancing factor and nonspecific cytotoxic cell receptor protein 1, whose transcript profiles were studied by qPCR. A consistency between lysozyme protein levels in the mucus and lysozyme mRNA levels in skin were found. Further research is necessary to unravel the implications of skin mucosal immunity on fish welfare and disease. Biological significanceThe present work reveals the proteomic changes, which are taking place in the skin mucus of stressed and non-stressed gilthead seabream after Pdp11 probiotic intake. The study contributes to improve the knowledge on skin mucosal immunology of this relevant farmed fish species.Furthermore, the paper shows for the first time how a suitable proteomic methodology, in this case 2-DE followed by LC-MS/MS is useful to perform a comparative study with a noninvasive technique of skin mucus of gilthead seabream.

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“…The intestine is one of the largest immune organs [1] and an important site for digestion/absorption of nutrients for fish [2]. However, the dynamics of nutrient transport in the intestine results in antigens exposing to intestinal mucosal [3], thereby leading to immune response [4].…”

Section: Introductionmentioning

confidence: 99%

The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine

Luo

Feng

Jiang³

et al. 2014

Fish & Shellfish Immunology

157

113

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Transcriptional regulation of cytokines in the intestine of Atlantic cod fed yeast derived mannan oligosaccharide or β-Glucan and challenged with Vibrio anguillarum

Cited by 119 publications

References 39 publications

Fish Nutrition and Current Issues in Aquaculture: The Balance in Providing Safe and Nutritious Seafood, in an Environmentally Sustainable Manner

Fish Nutrition and Current Issues in Aquaculture: The Balance in Providing Safe and Nutritious Seafood, in an Environmentally Sustainable Manner

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine

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