Transcriptional Regulation of Genes Encoding Key Enzymes of Abscisic Acid Metabolism During Melon (Cucumis melo L.) Fruit Development and Ripening

Sun, Yufei; Chen, Pei; Duan, Chongwen; Pang, Tao; Wang, Yanping; Jiao, Kai; Hu, Yin Gang; Li, Qian; Dai, Shengjie; Wu, Yan; Luo, Hao; Sun, Liang; Leng, Ping

doi:10.1007/s00344-012-9293-5

Cited by 47 publications

(25 citation statements)

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“…In this study, the upregulated expression of NCED2 genes in COS and LSW177 was found to aid the shift from carotenoid metabolism to ABA metabolism during fruit development depending on the increase in ABA content during watermelon fruit growth and ripening, which as previously mentioned [41], might be the main driver of cultivated watermelon maturation. The NCED gene also plays a primary function in the biosynthesis of ABA during fruit maturation [19, 36] in tomato [43] and melon [44]. Thus, NCED might be a noteworthy regulator of the ripening phase in cultivated watermelon.…”

Section: Discussionmentioning

confidence: 99%

“…A previous study showed that ClBGs with differential expression patterns in watermelon appear to have overlapping functions in ABA catabolism during watermelon ripening [41]. However, the expression of BG remained at its peak from the coloration step to fruit ripening to regulate the levels of ABA during melon and grape ripening [44, 45], which indicates that BG has an important complex function during watermelon ripening. However, the precise contribution of BG to the ABA levels in watermelon fruit requires further study.…”

Section: Discussionmentioning

confidence: 99%

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Comparative transcriptome analysis of two contrasting watermelon genotypes during fruit development and ripening

et al. 2017

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BackgroundWatermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] is an economically important crop with an attractive ripe fruit that has colorful flesh. Fruit ripening is a complex, genetically programmed process.ResultsIn this study, a comparative transcriptome analysis was performed to identify the regulators and pathways that are involved in the fruit ripening of pale-yellow-flesh cultivated watermelon (COS) and red-flesh cultivated watermelon (LSW177). We first identified 797 novel genes to extend the available reference gene set. Second, 3958 genes in COS and 3503 genes in LSW177 showed at least two-fold variation in expression, and a large number of these differentially expressed genes (DEGs) during fruit ripening were related to carotenoid biosynthesis, plant hormone pathways, and sugar and cell wall metabolism. Third, we noted a correlation between ripening-associated transcripts and metabolites and the key function of these metabolic pathways during fruit ripening.ConclusionThe results revealed several ripening-associated actions and provide novel insights into the molecular mechanisms underlying the regulation of watermelon fruit ripening.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3442-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis of two contrasting watermelon genotypes during fruit development and ripening

et al. 2017

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“…There have been a number of studies that have documented the metabolic changes that occur during maturation and ripening (Fait et al, 2008; Osorio et al, 2011,2012), though the link between hormonal control and metabolite accumulation is limited; however, Johnston et al (2009) observed in apple that, while this could progress independently of ethylene, it was highly sensitive to ethylene. In melon, the application of exogenous ABA was shown to promote starch hydrolysis (Sun et al, 2012b), different from growth section, however, this was confounded by the fact that the ABA also increased the ethylene levels.…”

Section: Fruit Ripening/senescencementioning

confidence: 99%

A dynamic interplay between phytohormones is required for fruit development, maturation, and ripening

McAtee¹,

Karim²,

Schaffer³

et al. 2013

Front. Plant Sci.

377

298

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Plant species that bear fruit often utilize expansion of an ovary (carpel) or accessory tissue as a vehicle for seed dispersal. While the seed(s) develop, the tissue(s) of the fruit follow a common progression of cell division and cell expansion, promoting growth of the fruit. Once the seed is fully developed, the fruit matures and the surrounding tissue either dries or ripens promoting the dissemination of the seed. As with many developmental processes in plants, plant hormones play an important role in the synchronization of signals between the developing seed and its surrounding fruit tissue(s), regulating each phase of fruit development. Following pollination, fruit set is achieved through a de-repression of growth and an activation of cell division via the action of auxin and/or cytokinin and/or gibberellin. Following fruit set, growth of the fruit is facilitated through a relatively poorly studied period of cell expansion and endoreduplication that is likely regulated by similar hormones as in fruit set. Once the seeds reach maturity, fruit become ready to undergo ripening and during this period there is a major switch in relative hormone levels of the fruit, involving an overall decrease in auxin, gibberellin, and cytokinin and a simultaneous increase in abscisic acid and ethylene. While the role of hormones in fruit set and ripening is well documented, the knowledge of the roles of other hormones during growth, maturation, and some individual ripening components is sketchy.

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“…ABA levels are increased via two biosynthetic mech-anisms. One mechanism involves de novo biosynthesis of ABA (Marin et al, 1996;Audran et al, 1998;Sun et al, 2013). The other biosynthetic process involves one-step hydrolysis of Glc-conjugated ABA by a BG (Dietz et al, 2000;Lee et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

The role of FaBG3 in fruit ripening and B. cinerea fungal infection of strawberry

Jiao

Sun

et al. 2013

The Plant Journal

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These authors contributed equally to this work. SUMMARYIn plants, b-glucosidases (BG) have been implicated in developmental and pathogen defense, and are thought to take part in abscisic acid (ABA) synthesis via hydrolysis of ABA glucose ester to release active ABA; however, there is no genetic evidence for the role of BG genes in ripening and biotic/abiotic stress in fruits. To clarify the role of BG genes in fruit, eight Fa/FvBG genes encoding b-glucosidase were isolated using information from the GenBank strawberry nucleotide database. Of the Fa/FvBG genes examined, expression of FaBG3 was the highest, showing peaks at the mature stage, coincident with the changes observed in ABA content. To verify the role of this gene, we suppressed the expression of FaBG3 via inoculation with Agrobacterium tumefaciens containing tobacco rattle virus carrying a FaBG3 fragment (RNAi). The expression of FaBG3 in FaBG3-RNAi-treated fruit was markedly reduced, and the ABA content was lower than that of the control. FaBG3-RNAi-treated fruit did not exhibit full ripening, and were firmer, had lower sugar content, and were pale compared with the control due to down-regulation of ripening-related genes. FaBG3-RNAi-treated fruit with reduced ABA levels were much more resistant to Botrytis cinerea fungus but were more sensitive to dehydration stress than control fruit. These results indicate that FaBG3 may play key roles in fruit ripening, dehydration stress and B. cinerea fungal infection in strawberries via modulation of ABA homeostasis and transcriptional regulation of ripening-related genes.

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Transcriptional Regulation of Genes Encoding Key Enzymes of Abscisic Acid Metabolism During Melon (Cucumis melo L.) Fruit Development and Ripening

Cited by 47 publications

References 50 publications

Comparative transcriptome analysis of two contrasting watermelon genotypes during fruit development and ripening

Comparative transcriptome analysis of two contrasting watermelon genotypes during fruit development and ripening

A dynamic interplay between phytohormones is required for fruit development, maturation, and ripening

The role of FaBG3 in fruit ripening and B. cinerea fungal infection of strawberry

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