Transcriptional regulation of iNOS and COX-2 by a novel compound from Curcuma comosa in lipopolysaccharide-induced microglial activation

Thampithak, Anusorn; Jaisin, Yamaratee; Meesarapee, Benjawan; Chongthammakun, Sukumal; Piyachaturawat, Pawinee; Govitrapong, Piyarat; Supavilai, Porntip; Sanvarinda, Yupin

doi:10.1016/j.neulet.2009.06.094

Cited by 22 publications

(15 citation statements)

References 26 publications

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“…Compound 092 treatment alone did not affect the level of NO (Anusorn Thampithak (AT) and Yupin Sanvarinda (YS), data not shown). The effect on NO production accompanied with suppression of iNOS mRNA and protein expression suggests the suppression role of compound 092 on LPS‐induced responses, which is consistent with findings in other experimental models …”

Section: Discussionsupporting

confidence: 90%

“…The effect on NO production accompanied with suppression of iNOS mRNA and protein expression suggests the suppression role of compound 092 on LPS-induced responses, which is consistent with findings in other experimental models. [15,22] Expression of the iNOS gene can be regulated by several pathways including NF-кB and Jak-STATs. [23] These pathways are targeted for treatment of inflammatory diseases.…”

Section: Discussionmentioning

confidence: 99%

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Concurrent suppression of NF-κB, p38 MAPK and reactive oxygen species formation underlies the effect of a novel compound isolated from Curcuma comosa Roxb. in LPS-activated microglia

Jiamvoraphong

Jantaratnotai

Sanvarinda

et al. 2017

Journal of Pharmacy and Pharmacology

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Concurrent suppression of NF-κB, p38 MAPK and reactive oxygen species formation underlies the effect of a novel compound isolated from Curcuma comosa Roxb. in LPS-activated microglia

Jiamvoraphong

Jantaratnotai

Sanvarinda

et al. 2017

Journal of Pharmacy and Pharmacology

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“…19−22 These cells have been shown to respond to the endotoxin LPS to induce protein S -nitrosylation. 9,23−25 …”

Section: Resultsmentioning

confidence: 99%

Proteomic Quantification and Site-Mapping of S-Nitrosylated Proteins Using Isobaric iodoTMT Reagents

Qu¹,

Meng²,

et al. 2014

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S-Nitrosylation is a redox-based protein post-translational modification in response to nitric oxide signaling and is involved in a wide range of biological processes. Detection and quantification of protein S-nitrosylation have been challenging tasks due to instability and low abundance of the modification. Many studies have used mass spectrometry (MS)-based methods with different thiol-reactive reagents to label and identify proteins with S-nitrosylated cysteine (SNO-Cys). In this study, we developed a novel iodoTMT switch assay (ISA) using an isobaric set of thiol-reactive iodoTMTsixplex reagents to specifically detect and quantify protein S-nitrosylation. Irreversible labeling of SNO-Cys with the iodoTMTsixplex reagents enables immune-affinity detection of S-nitrosylated proteins, enrichment of iodoTMT-labeled peptides by anti-TMT resin, and importantly, unambiguous modification site-mapping and multiplex quantification by liquid chromatography–tandem MS. Additionally, we significantly improved anti-TMT peptide enrichment efficiency by competitive elution. Using ISA, we identified a set of SNO-Cys sites responding to lipopolysaccharide (LPS) stimulation in murine BV-2 microglial cells and revealed effects of S-allyl cysteine from garlic on LPS-induced protein S-nitrosylation in antioxidative signaling and mitochondrial metabolic pathways. ISA proved to be an effective proteomic approach for quantitative analysis of S-nitrosylation in complex samples and will facilitate the elucidation of molecular mechanisms of nitrosative stress in disease.

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“…Our recent studies also showed that both Curcuma comosa hexane extract and its isolated compound, diarylheptanoid-049, are potent anti-inflammation agents. Curcuma comosa extract and diarylheptanoid-049 suppressed LPS-stimulated iNOS/COX2 mRNA and protein expression in microglia (Jantaratnotai et al, 2006;Thampithak et al, 2009). In the present study, it is not clear to what extent that anti-inflammatory action of Curcuma comosa extract participate in the protective effect against CCl 4 .…”

Section: Parametersmentioning

confidence: 98%

Protection of centrilobular necrosis by Curcuma comosa Roxb. in carbon tetrachloride-induced mice liver injury

Weerachayaphorn

Chuncharunee

Jariyawat

et al. 2010

Journal of Ethnopharmacology

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Transcriptional regulation of iNOS and COX-2 by a novel compound from Curcuma comosa in lipopolysaccharide-induced microglial activation

Cited by 22 publications

References 26 publications

Concurrent suppression of NF-κB, p38 MAPK and reactive oxygen species formation underlies the effect of a novel compound isolated from Curcuma comosa Roxb. in LPS-activated microglia

Concurrent suppression of NF-κB, p38 MAPK and reactive oxygen species formation underlies the effect of a novel compound isolated from Curcuma comosa Roxb. in LPS-activated microglia

Proteomic Quantification and Site-Mapping of S-Nitrosylated Proteins Using Isobaric iodoTMT Reagents

Protection of centrilobular necrosis by Curcuma comosa Roxb. in carbon tetrachloride-induced mice liver injury

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