Transcriptional regulation of meiosis in budding yeast

Kassir, Yona; Adir, Noam; Boger-Nadjar, E.; Raviv, Noga Guttmann; Rubin-Bejerano, Ifat; Sagee, Shira; Shenhar, Galit

doi:10.1016/s0074-7696(05)24004-4

Cited by 131 publications

(186 citation statements)

References 185 publications

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“…This includes repression of mitotic genes incompatible with meiotic development, activation of genes specifically needed for meiosis and gametogenesis, and adaptation of cell cycle-regulated loci to the meiotic divisions. Although such regulatory functions have typically been attributed to DNA binding transcriptional activators and repressors (27,28), a number of mitotic ncRNAs were recently shown to positively or negatively influence gene expression via changes of histone modification or transcriptional interference (17,(29)(30)(31)(32). It is therefore conceivable that developmentally regulated ncRNAs such as MUTs contribute to the control of genes required for, for example, cytokinesis (CHS2) and the mitotic cell cycle (CLN3, HUG1) through similar mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Execution of the meiotic noncoding RNA expression program and the onset of gametogenesis in yeast require the conserved exosome subunit Rrp6

Lardenois

Liu

Walther

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

126

217

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Budding yeast noncoding RNAs (ncRNAs) are pervasively transcribed during mitosis, and some regulate mitotic protein-coding genes. However, little is known about ncRNA expression during meiotic development. Using high-resolution profiling we identified an extensive meiotic ncRNA expression program interlaced with the protein-coding transcriptome via sense/antisense transcript pairs, bidirectional promoters, and ncRNAs that overlap the regulatory regions of genes. Meiotic unannotated transcripts (MUTs) are mitotic targets of the conserved exosome component Rrp6, which itself is degraded after the onset of meiosis when MUTs and other ncRNAs accumulate in successive waves. Diploid cells lacking Rrp6 fail to initiate premeiotic DNA replication normally and cannot undergo efficient meiotic development. The present study demonstrates a unique function for budding yeast Rrp6 in degrading distinct classes of meiotically induced ncRNAs during vegetative growth and the onset of meiosis and thus points to a critical role of differential ncRNA expression in the execution of a conserved developmental program.

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Section: Discussionmentioning

confidence: 99%

Execution of the meiotic noncoding RNA expression program and the onset of gametogenesis in yeast require the conserved exosome subunit Rrp6

Lardenois

Liu

Walther

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

126

217

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“…The down-regulation of Gsk3b is interesting, since its homolog gene in Saccharomyces cerevisiae, Rim11p (regulator of inducer of meiosis), is pivotal for the control of meiosis entry in response to nutritional signals (RubinBejerano et al, 2004;Kassir et al, 2003). Moreover, in vitro experiments have shown that Gsk3b inhibitors suppress DNA synthesis selectively in rat preleptotene spermatocytes, suggesting that Gsk3b might be required for the last round of DNA synthesis preceding meiosis (Guo et al, 2003).…”

Section: Control Of Differentiation and Meiotic Entrymentioning

confidence: 99%

“…Indeed, in S. cerevisiae, nutritional signaling pathways converge on transcriptional activation of the Ime1 gene, which encodes a transcription factor that, in turn, triggers initiation of meiosis, by turning off the expression of genes required for the mitotic cell cycle, while activating the expression of other genes which are crucial for execution of the meiotic program (Kassir et al, 2003). Thus, it is tempting to speculate that one of the effects of KL/kit interaction in differentiating spermatogonia is to establish progressively, during their mitotic divisions, a sort of endogenous state of nutritional stress, which might be the ancestrally inherited trigger for transcription of a master transcriptional regulator of meiosis.…”

Section: Metabolismmentioning

confidence: 99%

Transcriptome analysis of differentiating spermatogonia stimulated with kit ligand

Rossi

Lolicato

Grimaldi

et al. 2008

Gene Expression Patterns

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Kit ligand (KL) is a survival factor and a mitogenic stimulus for differentiating spermatogonia. However, it is not known whether KL also plays a role in the differentiative events that lead to meiotic entry of these cells. We performed a wide genome analysis of difference in gene expression induced by treatment with KL of spermatogonia from 7-day-old mice, using gene chips spanning the whole mouse genome. The analysis revealed that the pattern of RNA expression induced by KL is compatible with the qualitative changes of the cell cycle that occur during the subsequent cell divisions in type A and B spermatogonia, i.e. the progressive lengthening of the S phase and the shortening of the G2/M transition. Moreover, KL up-regulates in differentiating spermatogonia the expression of early meiotic genes (for instance: Lhx8, Nek1, Rnf141, Xrcc3, Tpo1, Tbca, Xrcc2, Mesp1, Phf7, Rtel1), whereas it down-regulates typical spermatogonial markers (for instance: Pole, Ptgs2, Zfpm2, Egr2, Egr3, Gsk3b, Hnrpa1, Fst, Ptch2). Since KL modifies the expression of several genes known to be up-regulated or down-regulated in spermatogonia during the transition from the mitotic to the meiotic cell cycle, these results are consistent with a role of the KL/kit interaction in the induction of their meiotic differentiation.

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“…Five of the proteins required for DSB formation (Spo11, Mei4, Rec102, Rec104, and Rec114) are meiosis-specific and their expression is controlled primarily by transcription (reviewed in Kassir et al 2003). Mer2 (also known as Rec107) is also meiotically induced, but its expression is controlled differently.…”

Section: Introductionmentioning

confidence: 99%

Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae

et al. 2007

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In most sexually reproducing organisms, meiotic recombination is initiated by DNA double-strand breaks (DSBs) formed by the Spo11 protein. In budding yeast, nine other proteins are also required for DSB formation, but roles of these proteins and interactions among them are poorly understood. We report here further studies of the behaviors of these proteins. Consistent with other studies, we find that Mei4 and Rec114 bind to chromosomes from leptonema through early pachynema. Both proteins showed only limited colocalization with the meiotic cohesin subunit Rec8, suggesting that Mei4 and Rec114 associated preferentially with chromatin loops. Rec114 localization was independent of other DSB factors, but Mei4 localization was strongly dependent on Rec114 and Mer2. Systematic deletion analysis identified protein regions important for a previously described two-hybrid interaction between Mei4 and Rec114. We also report functional characterization of a previously misannotated 5′ coding exon of REC102. Sequences encoded in this exon are essential for DSB formation and for Rec102 interaction with Rec104, Spo11, Rec114, and Mei4. Finally, we also examined genetic requirements for a set of previously described two-hybrid interactions that can be detected only when the reporter strain is induced to enter meiosis. This analysis reveals new functional dependencies for interactions among the DSB proteins. Taken together, these studies support the view that Mei4, Rec114, and Mer2 make up a functional subgroup that is distinct from other subgroups of the DSB proteins: Spo11-Ski8, Rec102-Rec104, and Mre11-Rad50-Xrs2. These studies also suggest that an essential function of Rec102 and Rec104 is to connect Mei4 and Rec114 to Spo11.

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Transcriptional regulation of meiosis in budding yeast

Cited by 131 publications

References 185 publications

Execution of the meiotic noncoding RNA expression program and the onset of gametogenesis in yeast require the conserved exosome subunit Rrp6

Execution of the meiotic noncoding RNA expression program and the onset of gametogenesis in yeast require the conserved exosome subunit Rrp6

Transcriptome analysis of differentiating spermatogonia stimulated with kit ligand

Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae

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