Transcriptional Regulation of Plant Biomass Degradation and Carbohydrate Utilization Genes in the Extreme Thermophile
            <i>Caldicellulosiruptor bescii</i>

Rodionov, Dmitry A.; Rodionova, Irina A.; Rodionov, Vladimir; Arzamasov, Aleksandr A.; Zhang, Ke; Rubinstein, Gabriel M.; Tanwee, Tania N. N.; Bing, Ryan G.; Crosby, James R.; Nookaew, Intawat; Basen, Mirko; Brown, Steven D.; Wilson, Charlotte M.; Klingeman, Dawn M.; Poole, Farris L.; Zhang, Ying; Kelly, Robert M.; Adams, Michael W. W.

doi:10.1128/msystems.01345-20

Cited by 14 publications

(8 citation statements)

References 75 publications

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“…2b , ABC transporters comprise multiple domains: an extra-cytoplasmic substrate-binding domain responsible for sugar-specific binding, a membrane spanning domain, and a nucleotide-binding domain (ATPase) (Ehrmann et al., 1998 ; Schneider & Hunke, 1998 ; Schneider, 2001 ; Hollenstein et al., 2007 ; Holland, 2019 ). While membrane-spanning and substrate-binding domains typically sit adjacent to one another in the same genetic locus, ATP-binding domains are sometimes located elsewhere in the genome due to their multifunctionality (Rodionov et al., 2021 ). As the naming suggests, unidirectional sugar transport is energetically coupled to ATP hydrolysis, typically at a ratio of two ATP molecules to one sugar molecule (Saurin et al., 1999 ).…”

Section: Sugar Transport In Thermophilesmentioning

confidence: 99%

Sugar transport in thermophiles: Bridging lignocellulose deconstruction and bioconversion

Tjo,

Conway

2024

Journal of Industrial Microbiology and Biotechnology

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Biomass degrading thermophiles play an indispensable role in building lignocellulose-based supply chains. They operate at high temperatures to improve process efficiencies and minimize mesophilic contamination, can overcome lignocellulose recalcitrance through their native carbohydrate-active enzyme (CAZyme) inventory, and can utilize a wide range of sugar substrates. However, sugar transport in thermophiles is poorly understood and investigated, as compared to enzymatic lignocellulose deconstruction and metabolic conversion of sugars to value-added chemicals. Here, we review the general modes of sugar transport in thermophilic bacteria and archaea, covering the structural, molecular, and biophysical basis of their high-affinity sugar uptake. We also discuss recent genetic studies on sugar transporter function. With this understanding of sugar transport, we discuss strategies for how sugar transport can be engineered in thermophiles, with the potential to enhance the conversion of lignocellulosic biomass into renewable products. One-Sentence Summary Sugar transport is the understudied link between extracellular biomass deconstruction and intracellular sugar metabolism in thermophilic lignocellulose bioprocessing.

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Section: Sugar Transport In Thermophilesmentioning

confidence: 99%

Sugar transport in thermophiles: Bridging lignocellulose deconstruction and bioconversion

Tjo,

Conway

2024

Journal of Industrial Microbiology and Biotechnology

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“…When genomic information is available, it acts as a blueprint where the pathways expressed under biomass-derived substrates can be traced in an experimental manner. Rodionov et al [ 112 ] determined, through transcriptome sequencing (RNA-seq), the expressed genes of thermophilic bacteria Caldicellulosiruptor bescii when growing on substrates of different degrees of complexity (from glucose to xylan), representing the main carbon sources obtained from plant biomass deconstruction. This allowed the assembly of exoenzymes, transporters, activators, and repressors, as well as biochemical pathways, either common or specific to the tested substrates, as well as the construction of regulons, their promoters, and regulatory DNA sequences.…”

Section: Strain Improvement By Characterization Of Integral Control O...mentioning

confidence: 99%

Engineering the Metabolic Landscape of Microorganisms for Lignocellulosic Conversion

Peña-Castro,

Muñoz-Páez,

Robledo-Narvaez

et al. 2023

Microorganisms

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Bacteria and yeast are being intensively used to produce biofuels and high-added-value products by using plant biomass derivatives as substrates. The number of microorganisms available for industrial processes is increasing thanks to biotechnological improvements to enhance their productivity and yield through microbial metabolic engineering and laboratory evolution. This is allowing the traditional industrial processes for biofuel production, which included multiple steps, to be improved through the consolidation of single-step processes, reducing the time of the global process, and increasing the yield and operational conditions in terms of the desired products. Engineered microorganisms are now capable of using feedstocks that they were unable to process before their modification, opening broader possibilities for establishing new markets in places where biomass is available. This review discusses metabolic engineering approaches that have been used to improve the microbial processing of biomass to convert the plant feedstock into fuels. Metabolically engineered microorganisms (MEMs) such as bacteria, yeasts, and microalgae are described, highlighting their performance and the biotechnological tools that were used to modify them. Finally, some examples of patents related to the MEMs are mentioned in order to contextualize their current industrial use.

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“…Extreme thermophilic bacteria belonging to the genus Caldeloulosiruptor degrade carbohydrates in the plant cell wall and subsequently utilize them. The mechanism of carbohydrate transcription regulation using genes and investigated the binding sites to TF and regulons with transcriptomic analysis for C. bescii cultivated on glucose, xylan, cellulose and cellobiose was observed (Rodionov et al 2021)…”

Section: Adapting Via Transcriptional Regulationmentioning

confidence: 99%

Microbial adaptation to different environmental conditions: molecular perspective of evolved genetic and cellular systems

et al. 2022

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Microorganisms are ubiquitous on Earth and can inhabit almost every environment. In a complex heterogeneous environment or in face of ecological disturbance, the microbes adjust to fluctuating environmental conditions through a cascade of cellular and molecular systems. Their habitats differ from cold microcosms of Antarctica to the geothermal volcanic areas, terrestrial to marine, highly alkaline zones to the extremely acidic areas, and freshwater to brackish water sources. The diverse ecological microbial niches are attributed to the versatile, adaptable nature under fluctuating temperature, nutrient availability, and pH of the microorganisms. These organisms have developed a series of mechanisms to face the environmental changes and thereby keep their role in mediate important ecosystem functions. The underlying mechanisms of adaptable microbial nature are thoroughly investigated at the cellular, genetic and molecular levels. The adaptation is mediated by a spectrum of processes like natural selection, genetic recombination, horizontal gene transfer, DNA damage repair, and pleiotropy-like events. This review paper provides the fundamentals insight about the microbial adaptability besides highlighting the molecular network of microbial adaptation under different environmental conditions.

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Transcriptional Regulation of Plant Biomass Degradation and Carbohydrate Utilization Genes in the Extreme Thermophile Caldicellulosiruptor bescii

Cited by 14 publications

References 75 publications

Sugar transport in thermophiles: Bridging lignocellulose deconstruction and bioconversion

Sugar transport in thermophiles: Bridging lignocellulose deconstruction and bioconversion

Engineering the Metabolic Landscape of Microorganisms for Lignocellulosic Conversion

Microbial adaptation to different environmental conditions: molecular perspective of evolved genetic and cellular systems

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